LGM International Inc., Ft. Lauderdale Second Generation of Liquid- -Based Cytology Based Cytology Second Generation of Liquid
Liqui-PREP TM System What makes LBC technology superior ?? What makes LBC technology superior ?? ▪ All Cells collected (100%) are preserved for analysis. ▪ Preservation & fixation of cells is immediate better preserving detailed nuclear morphology. ▪ Remove the blood and mucus, and clinically relevant cells are no longer occluded from field of vision (hidden cell). ▪ With clearer field of vision, slides can be scanned with greater accuracy (missed cell). “LBC Marketing Myth” ▪ HOWEVER, LBC is only as accurate as the cytologist who reads it!!!! ▪ LBC is a new tool for qualified cytologists to read with greater accuracy. 2
Liqui-PREP TM System Liqui- -PREP PREP TM Second Generation LBC Liqui TM Second Generation LBC 3
Liqui-PREP TM System LBC Products – – Market Evolution Market Evolution LBC Products First Generation Second Generation First Generation Second Generation � High cost pert test. � Low cost per test. � Special Equipment (capital � Standard centrifuge, vortex & funds). pipette. � Change in cellular morphology. � Classic morphology. � Specialized training to read. � Utilizes whole specimen. � Utilize only portion of specimen. � No filters to clog. � Bloody specimens/clogged � Separation chemistry to clean filters or acid pre-treatment. specimens. � HAZMAT disposal costs. � Non-HAZMAT formulations. � What is missed in residual vial? � Designed for non-gyn as well. 4
Collection Collection Choices Features ● Alcohol based- preserving standard cellular morphology ● Non-Hazmat – no exposure risk, lower shipping and disposal costs Endocervix brush ● Detachable brush head – assures all cells collected are fixed for analysis ● Room temperature storage ● Preserves cells, bacteria, protozoan, fungi, etc. for microscopic analysis Cervix Brush ● Protects and preserves nucleic acids and Preservative proteins allowing for subsequent follow-on Vial testing, i.e. HPV, Chlamydia, etc. All in One Combi Brush
Cleaning Solution 1,000g force-10 minutes Features: ALL cells collected are ► Whole specimen is processed poured onto the ► Utilizes separation chemistry to remove mucus Cleaning Solution ► Lytic Preservative lyses red blood cells ► Clinically relevant cells travel to bottom ► Bacteria, yeast, fungi, protozoan are preserved and captured in cell pellet
Slide Preparation Cell Base provides: ► adhesion to slide ► forms thin layer ► porous for rapid staining ► adjustable for various cell pellets ► set your own cell concentration Cell Base encapsulates cells, nucleic acids and proteins
Cell Base* Chemistry Classic morphology, i.e. honeycomb, Encapsulation of cells picket-fence, lesion aggregation/clumps, creates a density gradient are all maintained in the matrix, not for heavier cells to settle broken up by filters and cells seek space within the matrix Proprietary compound As matrix dries, pores forms an adhesive seal to form within the matrix to clean standard microscope allow stains, IHC to slides, avoiding expensive react on cell surfaces slide pretreatment Optimized Cell Density: No two specimens are alike, cell density/cell pellet ratio will impact final cell density on the slide. Cell Base can be optimized in a 1:2 to 1:8 ratio based upon cells collected to achieve the desired cell density, unlike a automated processor which dilutes all specimens the same. Graduated * Patents Pending Tube (ul)
Liqui-PREP TM System Benefits of LBC Benefits of LBC Conventional Pap Smear LBC (by Liqui-PREP TM ) 9
Sample Preps Negative within normal limits Endocervical Cells
Sample Preps Benign cellular changes Benign cellular changes associated with Herpes infection associated with Candida albicans
Sample Preps Squamous cell - ASCUS Squamous Cell - LSIL
Sample Preps Squamous cell Carcinoma Squamous Cell- HSIL
Sample Preps Thyroid Endocervical adenocarcinoma
Better Solutions through Better Chemistry!
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