CROATIAN VETERINARY INSTITUTE VPH dept. Laboratory for Food Microbiology Andrea Humski, Ph.D, DVM Marina Mikulić, DVM CROATIAN VETERINARY INSTITUTE
about CVI... Established in 1933. Leading national diagnostic, research and scientific institution in the field of veterinary medicine -Ministry of Agriculture, Fishery and Rural Development (MPRRR) -Ministry of Science, Education and Sports (MZOS) CROATIAN VETERINARY INSTITUTE
AREAS OF OPERATION 1. DIAGNOSTIC AND ANALYTICAL ACTIVITIES Preparedness, field and laboratory diagnostics, profilaxis, surveillance and monitoring, data analysis, planing and proposal of preventive actions, reference and scientific advisory functions in control of: • Infectious diseases • Zoonoses • Food and feed safety • Veterinary drugs and residues CROATIAN VETERINARY INSTITUTE
2. SCIENTIFIC AND RESEARCH ACTIVITIES Performed within the framework of The National scientific programme Executed through the several scientific research projects financially supported by Ministry of science, education and sport CROATIAN VETERINARY INSTITUTE
CVI laboratory network ...employees total no. – 238 • PhD – 34 • Msc – 13 • Sci. advisor – 7 • Senior sci. associate - 10 • Sci. associate – 11 CROATIAN VETERINARY INSTITUTE
CP Križevci Vinkovci CROATIAN VETERINARY INSTITUTE
CVI Zagreb I. VPH Dept. I.1. Laboratory for food microbiology I.2. Laboratory for residue control I.3. Laboratory for feed microbiology I.4. Laboratory for analytical chemistry I.5. Laboratory for veterinary medicinal products CROATIAN VETERINARY INSTITUTE
LABORATORY FOR FOOD MICROBIOLOGY, Zagreb • microbiological control of food: official control (import/export), on request (domestic production/market) • monitoring programmes – microbiological contamination of meat in slaughterhouses – microbiology food safety of products- veterinary inspection on border posts • research and scientific work CROATIAN VETERINARY INSTITUTE
LABORATORY FOR FOOD MICROBIOLOGY ACCREDITATION CERTIFICATE Microbiological parameter/method Enterobacteriaceae – Part 1.: MPN method 11 1 Salmonella spp. ISO 21528-1:2004 HRN EN ISO 6579:2003 / cor 2004 Salmonella spp. TECRA VIA – Screening method Enterobacteriaceae – Part 2.: Colony count 12 2 technique AOAC Official Method 989.14;998.09 ISO 21528-2:2004 Listeria monocytogenes – Part 1.: Detection method 3 Staphylococcus aureus and other species – Part 1.: 13 HRN EN ISO 11290 - 1:1999 Technique using Baird-Parker medium Listeria monocytogenes – Part 2.: Enumeration method 4 HRN EN ISO 6888-1:2004 HRN EN ISO 11290 - 2: 1999 Listeria spp. TECRA VIA – Screening method 5 14 Sulfite-reducing bacteria AOAC Official Method 995.22;2002.09 HRN ISO 15213:2004 6 Enumeration of microorganisms - CCT at 30°C 15 Clostridium perfringens - Colony count technique HRN ISO 4833:2003 HRN EN ISO 7937: 2005 Escherichia coli – Part 2.:Colony count technique at 44 °C 7 HRN ISO 16649-2:2001 Campylobacter spp. – Part 1. Detection method 16 Escherichia coli – Part 3.: MPN method 8 ISO 10272-1:2006 ISO/TS 16649-3:2005 Campylobacter spp. – Part 2. Enumeration method 17 9 Escherichia coli O157 ISO 10272-2:2006 HRN EN ISO 16654:2003 E. coli O157. TECRA VIA – screening method Campylobacter spp. TECRA VIA – 18 10 AOAC – RI, L.NO.001101 Screening method CROATIAN VETERINARY INSTITUTE
External Quality Assesment/Proficiency Testing Health Protection Agency, London, UK (since 2005.) Parameter No. of tested samples Salmonella spp. 15 Listeria monocytogenes / E 8 Listeria monocytogenes / D 4 E. coli , MPN 9 E. coli / E 2 E. coli O157 3 ACC 11 Campylobacter spp. 6 Coag. + Staphylococcus / E 5 Staph. aureus enterotoxin 2 Cl. perfringens / E 3 Enterobacteriaceae / E 3 CROATIAN VETERINARY INSTITUTE
Present and future activities…. CROATIAN VETERINARY INSTITUTE
Zoonoses in humans 2005 - 2008 Cases Deaths Rate per 100,000 population Rank Disease 2005 Salmonellosis 5619 0 124.9804 1 Leptospirosis 126 0 2.8026 2 Q fever 40 0 0.8897 3 Toxoplasmosis 39 0 0.8675 4 Trichinellosis 27 0 0.6005 5 Disease 2006 Cases Deaths Rate per 100,000 population Rank Salmonellosis 4734 1 105.2958 1 Trichinellosis 45 0 1.0009 2 Leptospirosis 39 0 0.8675 3 Toxoplasmosis 31 0 0.6895 4 Q fever 28 0 0.6228 5 Disease 2007 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3331 0 74.0897 1 Campylobacteriosis 141 0 3.1362 2 Leptospirosis 63 1 1.4013 3 Q fever 43 0 0.9564 4 Toxoplasmosis 31 0 0.6895 5 Disease 2008 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3664 0 81.4964 1 Campylobacteriosis 1294 0 28.7818 2 Q fever 41 0 0.9119 3 Brucellosis 25 0 0.5561 4 CROATIAN VETERINARY Toxoplasmosis 24 0 0.5338 5 INSTITUTE
6000 5500 Salmonellosis 5000 Campylobacteriosis 4500 4000 3500 3000 2500 2000 1500 1000 500 0 2005 2006 2007 2008 CROATIAN VETERINARY INSTITUTE
1. Monitoring programme - Ordinance on conduction of veterinary inspection and control of animal products in traffic across the border of the Republic of Croatia (O.G. 132/08) / Ordinance on the document for registration of veterinary health inspection of shipments of animal origin entering the Republic of Croatia (O.G.129/06 and 75/07) Annex II 2. Baseline study acc. to Commission Decision 2007/516/EC: Campylobacter and Salmonella prevalence in broiler flocks (carcasses + caecal content) CROATIAN VETERINARY INSTITUTE
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