Spring 2016 Principles of Neurophysiology (BIONB/BME/ECE 4910) - PowerPoint PPT Presentation

Spring 2016 Principles of Neurophysiology (BIONB/BME/ECE 4910) Lecture: 106 Comstock Hall; Lab: B150 Comstock Hall Web Site: http://courses.cit.cornell.edu/bionb4910/index.html *Blackboard * Instructors: Dr. Bruce Johnson, Yi-Yun Ho, Joannalyn Delacruz Teaching Assistant : Irwin Tendler

4910 Course Objectives 1. Gain an intuitive understanding for important concepts in Cellular Neurobiology. 2. Gain experience and competence in electrophysiological techniques (Job Skills!) 3. Understand and appreciate the experimental paradigms of the field. 4. Polish your scientific thinking and writing. 5. Appreciate the personal excitement of a research physiologist interacting with a living system. What’s a Model System? What are the main research model systems?

Fab 4 Research Model Systems (not to scale!) Why are invertebrate animals used as model systems to teach signal transmission in human nervous systems?

Reasons to use invertebrates for teaching neuroscience 1. Evolutionary conservation of excitability and synaptic transmission 2. Inexpensive to buy and maintain 3. No animal protocols (no IAUCUC) 4. Hardy nervous systems 5. Often cultured for food

Neuronal excitability and synaptic trans- mission changed little in a half-billion years 500 million "Never the less, all living things are about the same" C. Darwin Hille, 2001

Spring 2016 Schedule 0.4 Telson stimulated 0.2 0.0 Amplitude (mV) -0.2 -0.4 -0.6 -0.8 0 100 200 300 400 500 Time (ms)

4910 Schedule (cont)

Required “Texts” Wyttenbach, R.A., Johnson, B.R. and R.R. Hoy (2014) Crawdad: An Online Lab Manual for Neurophysiology . Sinauer Associates, Inc. Sunderland, MA. http://www.sinauer.com/crawdad-an-online-lab-manual-for-neurophysiology.html Moore, J.W. and A.E. Stuart (2007) Neurons in Action, Vers. 2 , Sinauer Associates, Inc., Sunderland, MA (http://www.sinauer.com/catalog/neuroscience/neurons- in-action-2-tutorials-and-simulations-using-neuron.html ) “Order from the website for a 15% discount from the suggested list price as reflected in “price to individuals” and free standard shipping to US addresses on orders over $40.00.*

4910 Grading A. ¡ 35%- Lab Reports B. ¡ 15%- Problem Sets and Other Assignments C. ¡ 20%- Midterm Paper D. ¡ 30%- Final Paper (25%) and Presentation (5%) Submit to BLACKBOARD!

Lab exercise Results Due Report Due

4910 Grading A. ¡ 35%- Lab Reports B. ¡ 15%- Problem Sets and Other Assignments C. ¡ 20%- Midterm Paper D. ¡ 30%- Final Paper (25%) and Presentation (5%) Blackboard; Neuronal Simulation Assignments Tutorial Assignment Page Due Date Assignment 1: Introduction to NIA 1 January 30 The Membrane Tutorial 9

4910 Grading A. ¡ 35%- Lab Reports B. ¡ 15%- Problem Sets and Other Assignments C. ¡ 20%- Midterm Paper D. 30%- Final Paper (25%) and Presentation (5%)

4910 Grading A. ¡ 35%- Lab Reports B. ¡ 15%- Problem Sets and Other Assignments C. ¡ 20%- Midterm Paper D. ¡ 30%- Final Paper (25%) and Presentation (5%) 20 mV Snail neuron 10 s Extra ¡credit ¡for ¡helping ¡lead ¡lab ¡exercises ¡for ¡an ¡Engineering ¡class!

Next week First Lab: Model Neuron membrane resistance, time constant, oscilloscope. LabChartsoftware tutorial