Glycopolymer Binding to SIGNR1, A Mouse Orthologue of Human DC-SIGN - PowerPoint PPT Presentation

Feb 07, 2024 •156 likes •317 views

Glycopolymer Binding to SIGNR1, A Mouse Orthologue of Human DC-SIGN M. Lougher Contents DC-SIGN Glycopolymers Why Use SIGNR1? Surface Plasmon Resonance Results Conclusions 2 DC-SIGN Membrane protein found in

Glycopolymer Binding to SIGNR1, A Mouse Orthologue of Human DC-SIGN M. Lougher
Contents • DC-SIGN • Glycopolymers • Why Use SIGNR1? • Surface Plasmon Resonance • Results • Conclusions 2
DC-SIGN • Membrane protein found in dendritic cells and some types of macrophages. • Receptor cells of this type traditionally bind to pathogens and present them to T-cells for destruction. • DC-SIGN binds to HIV and presents that to T- cells, but instead of being digested the HIV infects the T-cell. 1 3 1. H. Feinberg et al ., J. Mol. Biol., 394 , 613–620 (2009)
DC-SIGN • C-type lectin; binds to mannose rich pathogens. • GP120 in HIV envelope has mannose groups. • Aim to design prophylactic treatment that binds to the carbohydrate recognition domain (CRD) and prevents GP120 from binding. • One possible solution is glycopolymers. 1 4 1. H. Feinberg et al ., J. Mol. Biol., 394 , 613–620 (2009)
Glycopolymers • Polymer chain with sugar groups attached. • Used 3 different shape polymers each bound with multiple mannose groups. Straight Chain 8 Arm (DP60) 5 Arm 5
Why Use SIGNR1? • Mouse orthologue of DC-SIGN. • Test to see if experiments on mice would be relevant to research for human treatment. • Would allow research without using human tissue; much easier to carry out. • Need to test to see if binding of glycopolymers is comparable both in-vitro and in-vivo. 6
Surface Plasmon Resonance • Method of detecting binding to a thin gold surface as refractive index changes. 2 7 2. M. Cooper, Nature Reviews Drug Discovery 1 , 515-528 (2002)
Results • MAN DP60 8
Results • MAN-8ARM 9
Results • MAN-5ARM 10
Results • Regeneration 11
Results • Flow Cytometry 12
Results • Cytotoxicity 13
Summary • MAN DP60 shown to bind well to DC-SIGN and SIGNR1. • Further work needed for conclusive results of MAN-5ARM and MAN-8ARM. • Demonstrated cytokines increase binding to cells. • Shown that polymers are not toxic to cells in concentrations used. • Binding profiles similar for SIGNR1 and DC-SIGN; mouse disease models can be used to develop human treatment. 14
Acknowledgements • Thanks to: – Dan Mitchell – Remzi Becer – Tariq Pathan – Rob Deller – Florence Hariton • Funded by: – MOAC – EPSRC 15

Recommend

1.3 Mouse Training We are going to learn What a mouse is How to hold a mouse How to

1.3 Mouse Training We are going to learn What a mouse is How to hold a mouse How to use a mouse What is the Mouse? The mouse is an input device designed to manipulate objects on the computer screen and assist you, the user, in

161 views • 13 slides

Expression of human H ferritin prompts the identification of a hitherto elusive yeast orthologue

Expression of human H ferritin prompts the identification of a hitherto elusive yeast orthologue and enables parsing of distinct iron-induced cell death pathways in Saccharomyces cerevisiae. Rawan Eid 1,2 , Nagla T.T. Arab 1,2 , Chamel Khoury

401 views • 29 slides

Late binding Ch 15.3 Highlights - Late binding for variables - Late binding for functions

Late binding Ch 15.3 Highlights - Late binding for variables - Late binding for functions Review: Derived classes Today we will deal more with inheritance Mainly we will focus on how you can store a child class in a parent container (sort

550 views • 30 slides

The Binding Problem(s) 8/25/2010 9:38 AM Jerome Feldman Abstract The neural binding problem

The Binding Problem(s) 8/25/2010 9:38 AM Jerome Feldman Abstract The neural binding problem (NBP) encompasses three distinct situations: feature binding, variable binding, and the subjective unity of perception. Feature binding involves

160 views • 15 slides

The Mouse Genome The Mouse Genome Database (MGD) Database (MGD) Eppig J.T., et al. (2005). The

The Mouse Genome The Mouse Genome Database (MGD) Database (MGD) Eppig J.T., et al. (2005). The Mouse Genome Database (MGD): Eppig J.T., et al. (2005). The Mouse Genome Database (MGD): From genes to mice: A community resource for mouse biology.

443 views • 5 slides

Apple Magic Mouse Redesign 5.18.18 Reid Parsekian Background Background Magic Mouse Redesign

Apple Magic Mouse Redesign 5.18.18 Reid Parsekian Background Background Magic Mouse Redesign History The Lisa Mouse began as one of the first commercial mice available to consumers. Over the years Apple has maintained a distinct form and

575 views • 28 slides

Attention, Binding, and Consciousness 1. Perceptual binding, dynamic binding 2. Neural

4/22/17 Attention, Binding, and Consciousness 1. Perceptual binding, dynamic binding 2. Neural Correlates of Consciousness: Binocular rivalry 3. Attention vs. consciousness 4. Binding revisited: Split-brain, split-consciousness 2 1

404 views • 21 slides

Attention, Binding, and Consciousness 1. Perceptual binding, dynamic binding 2. Neural

4/18/17 Attention, Binding, and Consciousness 1. Perceptual binding, dynamic binding 2. Neural Correlates of Consciousness: Binocular rivalry 3. Attention vs. consciousness 4. Binding revisited: Split-brain, split-consciousness 2 1

534 views • 17 slides

Internal Chloride Binding of OPC Pastes: Modelling Using Binding Isotherms Dr. Dipl. Eng. M.V.A.

Internal Chloride Binding of OPC Pastes: Modelling Using Binding Isotherms Dr. Dipl. Eng. M.V.A. (Miruna) Florea L.C. (Lara) Quaas, MSc Prof. dr. ir. H.J.H. (Jos) Brouwers Sustainable energy in the Netherlands 10 Other biomass combustion 8 Co

248 views • 20 slides

Characterizing and Predicting Hexose-Binding Sites Houssam Nassif

Background Representation Glucose Binding Hexose Binding Rules Characterizing and Predicting Hexose-Binding Sites Houssam Nassif http://pages.cs.wisc.edu/~hous21/ CIBM Seminar 25 January 2011 Background Representation Glucose Binding

991 views • 76 slides

Android Data Binding: This is the DSL you're looking for Maksim Lin Freelance Android Developer

Android Data Binding: This is the DSL you're looking for Maksim Lin Freelance Android Developer www.manichord.com The plan Why Data Binding ? Brief intro to Data Binding Library Adding Data Binding to an existing app Two-way

719 views • 55 slides

DNA Binding Proteins CSE 527 Autumn 2007 A variety of DNA binding proteins (transcription

DNA Binding Proteins CSE 527 Autumn 2007 A variety of DNA binding proteins (transcription factors; a significant fraction, Lectures 8-9 (& part of 10) perhaps 5-10%, of all human proteins) Motifs: Representation & Discovery

625 views • 16 slides

W hen practitioners think of arbitration, they Binding usually mean a process that results in a

A R B I T R A T I O N NON-BINDING ARBITRATION: AN INTRODUCTION By Steven C. Bennett W hen practitioners think of arbitration, they Binding usually mean a process that results in a arbitration has final, binding and enforceable award,

260 views • 5 slides

Experimental binding Irene . Liyana . Shi Teng STAB . ACCORDION . SADDLE STITCH STAB BINDING

Experimental binding Irene . Liyana . Shi Teng STAB . ACCORDION . SADDLE STITCH STAB BINDING STAB BINDING Loose or folded individual sheets bound by stabbing holes and then sewing the loose edge together with thread. Types: Folded

578 views • 30 slides

Late binding Ch 15.3 Highlights - Late binding for variables Review: Derived classes Today we

Late binding Ch 15.3 Highlights - Late binding for variables Review: Derived classes Today we will deal more with inheritance Mainly we will focus on how you can store a child class in a parent container (sort of) Questions we will answer:

910 views • 18 slides

Dragging October 21, 2008 1 Dragging Rectangles User interface effect that we want: User

Dragging October 21, 2008 1 Dragging Rectangles User interface effect that we want: User depresses mouse button while mouse is over an rectangle to pick up the rectangle User drags mouse (that is, moves the mouse while holding the

321 views • 11 slides

Coexistence of Address Assignment Methods or HOW TO DEAL WITH BINDING COLLISIONS in an

IETF 77 Coexistence of Address Assignment Methods or HOW TO DEAL WITH BINDING COLLISIONS in an HETEROGENEOUS ENVIRONEMENT? What is a binding collision? Entry [ IP Address, vlan , anchor] exists in the binding table Collision happens

684 views • 12 slides

Binding in Java Beans Inspecting bean properties with a GUI binding veto GUI BEANS Pieter van

Binding in Java Beans HOM,DOS beans? convention Binding in Java Beans Inspecting bean properties with a GUI binding veto GUI BEANS Pieter van den Hombergh Summary on Java Beans FX Properties Thijs Dorssers Fontys Hogeschool voor

428 views • 18 slides

Fragment binding prediction using unsupervised learning of ligand substructure binding sites

Fragment binding prediction using unsupervised learning of ligand substructure binding sites Grace Tang Altman Lab July 20, 2013 Berlin, Germany 1 Structure Based Virtual Screening Virtual Databases End Goal Screening ZINC 1 21 million

491 views • 15 slides

Mouse Epithelium Dataset S IN GLE-CELL RN A-S EQ W ORK F LOW S IN R Fanny Perraudeau Senior

Mouse Epithelium Dataset S IN GLE-CELL RN A-S EQ W ORK F LOW S IN R Fanny Perraudeau Senior Data Scientist, Whole Biome Typical workow SINGLE-CELL RNA-SEQ WORKFLOWS IN R Stem Cell Differentiation in the Mouse Olfactory Epithelium 1 2

462 views • 18 slides

CIS II: Project 15 MOUSE OPTICAL PROPERTIES SUMMARY UPDATED: 4.13.2016 Notes and Conventions

CIS II: Project 15 MOUSE OPTICAL PROPERTIES SUMMARY UPDATED: 4.13.2016 Notes and Conventions Cow: Green Human: Blue Mouse: Red Pig: Black Rat: Purple Using the following parameters for Alexandrakis calculations: Prahl S A 2001

428 views • 16 slides

Sensitivity and Specificity of the Nude Mouse Bioassay to the Clinical Islet Allotransplantation

Sensitivity and Specificity of the Nude Mouse Bioassay to the Clinical Islet Allotransplantation Outcome. Klearchos Papas, PhD University of Minnesota Medical School Outline Islet product testing objectives Correlation of nude mouse

236 views • 10 slides

CSE 331 More Events (Mouse, Keyboard, Window, Focus, Change, Document ...) slides created by

CSE 331 More Events (Mouse, Keyboard, Window, Focus, Change, Document ...) slides created by Marty Stepp based on materials by M. Ernst, S. Reges, D. Notkin, R. Mercer, Wikipedia http://www.cs.washington.edu/331/ 1 Mouse events Usage of

633 views • 29 slides

Characterization, Modeling, and Characterization, Modeling, and Simulation Simulation of Mouse

Characterization, Modeling, and Characterization, Modeling, and Simulation Simulation of Mouse Microarray Microarray Data Data of Mouse David S. Lalush Bioinformatics Research Center North Carolina State University Acknowledgments

604 views • 58 slides