Carinata Summit 2016 Update on Breeding Efforts in Carinata
Growth of Agrisoma & Collaborator R&D teams ……………………………………………………………………………………………………… Agrisoma R&D Team Carinata “on the ground” R&D Teams Management Greenhouse INIA Uruguay - J. Klingenberg (VP Product - M. Troesch (GH Lead) Lizama Farms Chile Development) University of Florida - S. Klashinsky (GH) - M. Lindenbaum (VP Auburn University Technology) Field team South Dakota State University - D. Males (Director Breeding & - C. Bliss (Agronomist) North Dakota State University Agronomy) - M. Henke (Agronomist) Montana State University - M. Lortie (Director Regulatory) United States Department of Agriculture - K. Wilson (Agronomist) (various extensions) - R. Attri (Nursery Manager) Saskatoon labs Agriculture and Agri-Food Canada - R. Bennett (Breeder) National Research Council of Canada - S. Rooke (Asst. breeder) Agri-ARM Groups (Western Canada) - M. Moore (DH lead) Westwind Ag Research - K. Czechowicz (DH group) Farming Smarter - V. Catinot (DH group) Mustard 21 - T. Fowler (DH group) + Other groups involved in testing of oil, meal, - K. Livingstone (DH group) processing, etc. - E. Hill (Crossing specialist) - P. Fu (Genetic diversity) R&D efforts enhanced through collaborative effort and development of - E. Risseeuw (Molecular) local expertise in each geography - L. Chen (Molecular) - T. Hoffman (Analytics) 2
Progress in experimental line production ……………………………………………………………………………………………………… No. of DH lines field evaluated Target: Consistently produce 8-10K 9000 DH lines per year 8000 7000 Simultaneous, efficiency increased by removing lines 6000 at early stage using: 5000 i) Molecular markers (beginning to use first set of 4000 markers) 3000 2000 ii) Flow cytometer (removes haploid plants at plantlet stage) 1000 0 Project Total no. plantlets No. doubled Ratio fertile analyzed (2N) Cross set #1 10,341 5,443 52.6% Cross set #2 4,138 2,227 53.8% Cross set #3 10,157 3,205 31.6% Totals for 2015 24,636 10,875 44.1% DH team effort : Produce large number lines on front end to reach goals, increase chance of success Molecular team effort : Marker discovery phase, implement markers in high throughput system 3
Progress in Agronomic Potential of Experimental lines ……………………………………………………………………………………………………… 2014 Summer 2015 Summer 2015-16 Winter 2014 Summer 2015 Summer 2015-16 Winter 600 1300 Inbred selections DH lines 1200 550 No. of lines evaluated 1100 500 1000 450 900 400 800 350 700 300 600 250 500 200 400 150 300 100 200 50 100 0 0 5 or less 6 7 8 9 5 or less 6 7 8 9 Agronomic Impression Rating; Higher is better, Checks = ~7 Cycle Percent of total Cycle Percent of total rated 7 or better rated 7 or better 2014 Summer 25% 2014 Summer 7% 2015 Summer 40% 2015 Summer 31% 2015-16 Winter 87% 2015-16 Winter 83% 4
Progress in Test Hybrid production ……………………………………………………………………………………………………… No. of A-line Estimated genetic Current: selections distance from A120 • Generating seed for two R-lines and twenty A-lines for test 13 0.400 – 0.450 hybrid production 18 0.350 – 0.400 Summer 2016: 2 0.300 – 0.350 • Initiate production of test hybrid seed • Target: 20 A-lines x 2 R-lines = Seed for 40 test hybrids 0 0.250 – 0.300 produced 0 0.200 – 0.250 Winter 2016-17: 2 0.150 – 0.200 • Target: 20 additional A-lines x 2 R-lines = Seed for 40 1 <0.150 additional test hybrids produced 12 Data not available Summer 2017: Average all 0.392 • First set of 80 test hybrids in yield trials selections • 269 unlinked SNP loci used in analysis, representing all chromosomes • Higher fraction indicates greater dissimilarity 5
Progress in marker discovery: NAM project ……………………………………………………………………………………………………… Phenotyping beginning soon: 2016 N. Tier “test run” • 51 Founder Lines 2016-17 S. Tier • Inbred population phenotyping (2,500 lines Commercially relevant traits: Includes traits such as • Early vigour • Flowering, maturity • Branching • Thousand seed weight • Seed quality parameters • Etc. etc. 6
“Southern Tier” product development: SE United States and Uruguay ……………………………………………………………………………………………………… Three high priorities, besides yield per se : 1. Days to maturity 2. Frost tolerance 3. Very good shatter tolerance Uruguay Testing 2015 - La Estanzuela, Uruguay Entry Days to maturity (vs. Shatter loss estimates Yield estimate (kg ha -1 ) (kg ha -1 ) (% of yield) A110) (% vs. A110) 159-4A1D 175 (Even) 69 (1%) 6155 (126%) 044-312D 170 (-5 days) 45 (1%) 5680 (116%) 044-3B1 175 (Even) 231 (4%) 5371 (110%) 159-1N1A 175 (Even) 67 (1%) 5309 (109%) 02.023 169 (-6 days) 48 (1%) 5039 (103%) 945-Z.063 176 (+1 day) 174 (4%) 4900 (100%) A110 175 168 (3%) 4891 (100%) 044-3111 177 (+2 days) 355 (8%) 4555 (93%) 40.168 176 (+1 day) 272 (7%) 3806 (78%) 7
“Southern Tier” product development: Early maturity nursery ……………………………………………………………………………………………………… Cross Parental DTM vs. No. lines A110 in N. Tier #137 -1 x -5 28 #145 -7 x -3 237 #157 -5 x -1 522 • Earliest flowering 10% of lines from each population selected and self-pollinated • Goal for 2015- 16: Identify line with ≥ 7 days earlier maturity than A120 • Future crosses using parents evaluated in short Early maturity nursery, Feb 22, 2016 day, winter environment (vs. long day, summer in current nursery) 8
“Southern Tier” product development: Freeze tolerance ……………………………………………………………………………………………………… Frost tolerance ratings: Quincy 2014-15 Name Rating - Site 1 Rating - Site 2 868-22B 40 20 25.102 50 30 439-A1 50 30 859-1 50 20 349-M22B 50 30 207-112 50 40 044-3B1 60 30 949-BI 60 20 044-3111 60 40 044-312D 70 40 159-11A2 70 50 AAC A120 70 50 AAC A110 70 40 044-3A21 80 40 400-A13C 80 50 40.063 80 40 19.308 80 60 137-5.106 90 40 • Rating indicates estimate of % plot damaged, three weeks after hard frost More frost damage Less affected • Green indicates entries that were better than checks in FL 2013-14 trials 9
“Southern Tier” product development: Line ‘312D’ ……………………………………………………………………………………………………… • High yield potential variety in Florida and Uruguay testing • Six isolation fields of top selections in Chile, rogueing and colour sorting for more uniform type • Corresponding selections in 2015-16 FL yield trials Isolation field “M - 04”, Chile 2015 -16 Rogueing Crew, Isolation field “HP - 15” Seed harvested from Isolation field “M - 01” 10
“Southern Tier” product development: Line ‘312D’ ……………………………………………………………………………………………………… Trial % seed yield of current check variety 2014-15 Quincy, FL (No Cadre residue) 123% 2014-15 Quincy, FL (Cadre residue) 109% 2015 Uruguay yield trial 116% 2015 Uruguay strip trials (avg. 5 sites) 113% Isolation tent “HP - 06”, Chile 2015 -16 11
Future direction: Breeding efforts ……………………………………………………………………………………………………… Expand germplasm screening in Florida winter nursery: 2015-16 = 3 crosses, 800 lines screened for early maturity; 2016-17 = Two frost tolerant cross populations generated thus far Expand validation of germplasm in Uruguay yield testing: 2015 = 10 entries x 1 site; looking at an additional site in 2016? Numbers of DH lines produced for field testing will continue to increase Identification and use of molecular markers continuing to become more important part of program, efficient selection of lines going into field testing Collaboration among Agrisoma and many partners in a number of geographies makes the breeding program stronger; “eyes” on the ground and local expertise 12
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