Why I love my job: The enabling influence of breeding We are here Stan Allen ABC, VIMS
Biomass pyramid Breeding Genetic services Multiplier population Brood stock services Hatchery production Commercial grow out
Biomass pyramid Breeding Genetic services Multiplier population Brood stock services Hatchery production Commercial grow out Economic multiplier
Breeding Genetic services: MASS SELECTION 0 0
MASS SELECTION: Lines to Superlines in 2008 LINE ORIGIN GENES DDBY DEBY D FBST DEBY D,X ADMO DEBY D,W DBY ADMOW DEBY D,W ADXB DEBY,XB D,X ADXBW DEBY,XB D,X XB BXB XB X BXBW XB X CAMX XB,CAM X,L ZOBOY OBOY L ZOBOYX OBOY L LA ZLGT LGT L ZLGTK LGT L ZLGTW LGT L YDBLA DEBY,LGT D,L
MASS SELECTION: DR + growth rate Low DBY High Kinsale X XB High Lola X LA York hANA X Lynnhaven
Breeding Genetic services: FAMILY SELECTION 0 0
FAMILY SELECTION: Since 2010 ♂ brothers and sisters x ♀
FAMILY SELECTION: Test sights X Cambridge low X Lewisetta X York high ~ 150 families X Lynnhaven • ~150,000 oysters • ~750 reps deployed
FAMILY SELECTION: Correlation low (HPL) vs. high (VIMS) salinity Genetic correlation r g = 0.7 TOTAL WEIGHT LOW SALINITY TOTAL WEIGHT HIGH SALINITY
FAMILY SELECTION: Correlation low (HPL) vs. high (VIMS) salinity SURVIVAL: high - low salinity sites 1.5 SURVIVAL LOW SALINITY 1 Survival Low salinity (GSD) 0.5 0 -0.5 -1 -1.5 -1.5 -1 -0.5 0 0.5 1 SURVIVAL HIGH SALINITY Survival High salinity (GSD)
Breeding Genetic services: TETRAPLOID SELECTION 0 0
disease resistance 2n 4n 4 years 20+ years Breeding legacy
TETRAPLOID SELECTION: The fathers of triploids 4n breeding objectives Survival Growth rate
FAMILY SELECTION: Test sights X Cambridge X York ~ 50 families • ~50,000 oysters • ~250 reps deployed
Multiplier population Brood stock services: MASS SELECTION
MULTIPLIER POPULATION: Mass Selection distributed to hatcheries selected discards
Multiplier population Brood stock services: FAMILY SELECTION TETRAPLOID SELECTION
Top families Diploid: Lily, Hnry Tetraploid: FYR MULTIPLIER POPULATION: family breeding
Brood stock available from ABC Mass selection Family selection 2n 4n 2n 4n HNRY 4FYR (2019?) DEBY 4GEN LILY XB Lola 4NGN (2019) hANA 4GNL
Brood stock distributed by ABC Tetraploid Diploid 30 25 Thousands 20 15 10 5 07 09 10 12 13 15 16 17 18 08 11 14 Year
All brood stock (2n) SELECTION LINE S SITE ORIGIN ATTRIBUTES NOTES Founder population strarted by Allen at HSRL using Haskin lines in 1990s, then Mass selection for MSX, continuous exposure to selection in VA, new material added in ambient Dermo, seems to favor higher salinity XB 2008 York R. and Lynnhaven Wild Del Bay oysters selected at VIMS in Mass selection for MSX, continuous exposure to York by Burreson group in 80s, new ambient Dermo, general all purpose across material added in 2008 salinity ranges DEBY-Lynn 18-25 Founders from three wild populations in Mass selection for MSX, continuous exposure to Discontinued Louisiana ca. 2000-2004, new material ambient Dermo, grows (too?) fast, mortality in starting 2018 added in 2008 some places hANA DIPLOID Founders from wild and selected Family selection for high survival in high salinity, populations ca. 2005, family selection continuous exposure to ambient Dermo, co- Released 2017 program, top families form terminal selected for shape, growth rate, meat yield HNRY brood stock population Wild Del Bay oysters selected at VIMS in Mass selection for MSX before low salnity Discontinued ~15 York, then York by Burreson group in 80s, new selection began in 2008, low ambient Dermo starting 2018 material added in 2008 exposure, not in high demand DEBY-Lew Coan R. Founders from three wild populations in Mass selection for MSX up to 2008, then Choptank R. Md Coan R. Va Louisiana ca. 2000-2004, new material continuously held at low salinity, low exposure to added in 2008 Dermo, widely popular at various salinity Lola 8-15 Founders from wild and selected Family selection for high survival in low salinity, populations ca. 2005, family selection low exposure to ambient Dermo, co-selected for Released 2017 program, top families form terminal shape, growth ratem, meat yield LILY brood stock population
All brood stock (4n) SELECTION LINE S SITE ORIGIN ATTRIBUTES NOTES Original tetraploid line derived from spawns of disease resistant oysters ca. Highly domesticated, disease resistant,some York R. and Lynnhaven early 2000s and propagated for ~10 inbreeding, variable growth rates 4GEN --> 4NGN generations 18-25 4VBOY tetraploids (with Louisiana germ More robust, more uniform, must be made anew TETRAPLOID plasm) are hybridized with 4GENs to make each generation requiring maintenance of 4VBOY Released 2017 F 1 brood stock line 4GNL Choptank R. Breeding for usual traits, teraploid stability at high 8-12 salinity Md Founder populations from various old and Likely line release 4FML new tetraploid lines contributed to 2019 tetraploid family breeding program Breeding for usual traits, teraploid stability at low 18-22 York R. Va salinity
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