an american bullfrog in brazil
play

An American Bullfrog in Brazil Lauren V. Ash University of Vermont - PowerPoint PPT Presentation

An American Bullfrog in Brazil Lauren V. Ash University of Vermont June 4, 2019 Background The American Bullfrog ( Lithobates catesbeianus ) Background Hoverman et al. 2011 Background High viral loads? Background Are invasive Bullfrogs in


  1. An American Bullfrog in Brazil Lauren V. Ash University of Vermont June 4, 2019

  2. Background The American Bullfrog ( Lithobates catesbeianus )

  3. Background Hoverman et al. 2011

  4. Background High viral loads?

  5. Background Are invasive Bullfrogs in Brazil harboring the same levels of ranavirus? Dr. Karla Dr. Domingos Campião, Rodrigues, UFPR UFMT Hypothesis: Ranaviruses have spread beyond the Bullfrog facilities We predict Bullfrogs will harbor the virus in high levels, similar to their native counterparts

  6. Background An extra layer: helminths Not much is understood regarding helminth Ancanthocephalins/thorn parasite dynamics and y headed worms diversity Nematodes/ro Platyhelminthes/flatwor Caused by trematode undworms ms ( Ribeiroia sp .)

  7. Background Is there an association between ranavirus and helminth parasites? 35 35 6 30 30 5 25 25 4 20 20 3 15 15 2 10 10 1 5 5 0 0 0 Yes No Yes No Yes No More parasites in More parasites in No difference Ranavirus positive Ranavirus negative vs

  8. Background Methods Brazil Maps

  9. Background Methods

  10. Background Methods Methods

  11. Background Methods

  12. Background Methods Results 8/9 ~89% 3/3 ~100% 1/9 ~11%

  13. Background Background Methods Methods Results Results Site Prevalence

  14. Background Background Methods Methods Results Results Viral copies per uL

  15. Background Methods Results Parasite Abundance

  16. Background Methods Results Parasite Abundance and viral load

  17. Background Methods Results Conclusions Summary • The first comprehensive survey and one of the first records of ranavirus in natural amphibian populations in Brazil • Detected low level infections in Brazilian adult Bullfrog populations • Individuals and sites with ranavirus vs presence seem to have decreased numbers of parasites but does not correlate to the amount of virus

  18. Background Methods Results Conclusions Next Steps • Are the sites that are ranavirus positive close to active Bullfrog farms? • Sequencing to determine which ranavirus species • Is this impacting native anurans in Brazil?

  19. ACKNOWLEDGEMENTS Every The Gotelli lab Funding volunteer! Landowners John Wheeler Award UVM Biology faculty Graduate College Summer Fellowship and graduate students The Ronald Suiter Prize All of my undergraduate research Dr. Roberto Fabri Fialho Award assistants

  20. Questions? @lvash23 Email: lvash@uvm.edu

Recommend


More recommend