Valorising g biopo polymers f from an animal w was aste te: - PowerPoint PPT Presentation

Valorising g biopo polymers f from an animal w was aste te: collagen, e elas asti tin and k kerati tin QuaPA UR370 & BIA UR1268 (May 2015 – April 2017) Vincen enza za Fer errar aro AgreenSkil ills ls Annu nual Meet eeting, 12 12 th th - 15 15 th th Octobe ber 2015 15 Barcelona ona, S , Spain

 PhD in Biotechnology, Universidade Católica Portuguesa (FP6 Marie Curie Actions)  Master Degree in Chemical Engineering, Universitá degli Studi di Salerno , Italy  Valorisation of agro-food waste through the recovery of high-added value compounds  Food Safety and Food Security  Food Processing Engineering  International research projects (17 countries)  Academy to industry knowledge transfer

 Recovery of the structural fibrous proteins collagen, elastin and keratin, and respective hydrolysates and small peptides from animal waste , and  application in biomaterials, pharmaceutics, cosmetics and food  Add commercial value to a waste material currently underutilized in fine chemistry  Contribute to the sustainability of the agro-food sector  Reduce impact of waste on the environment

Respective protopeptide in the form of α -helix having specific amino acids • heptad motifs and responsible for a spontaneous self-assembly into tridimensional networks Functional biopolymers which allow conceiving formulations for the bio-based • industry (medicine, pharmaceutics, cosmetics and food) The hydrolysis through enzymes, chemicals or thermal treatment, gives rise to • biologically active peptides (2-30 amino acids residues) which can modulate numerous physiological pathways …some examples of application…

Elastin from LIGAMENTS Collagen from cortical BONES Keratin from FEATHERS Morphology of the fibres (Electron Microscopy at INRA, QuaPA) and supramolecular structure

On average, 1/3 of food is currently wasted worldwide Million Tons/year Protein-containing waste Polysaccharide-containing waste Lower amount but difficult Higher amount but easier management, slower degradation management, low impact and and very high costs of disposal low to zero costs of disposal (25–70 EUR/ton)

Old (7 y.) and Young (4 y.) Femur and Tibia Old (7 y.) and Young (4 y.) Femur and Tibia METHODOLOGY RESULTS Bone milling Bone Acid extraction Collagen 60 T.Y. 50 F.Y. T.O. Spray-drying 40 Yield (%) F.O. 30 20 10 0 Bone

Young Femur (4 y.) Old Femur (7 y.) Old Tibia (7 y.) Young Tibia (4 y.)

o Review submitted to Trends in Food Science & Technology The “sisters” α -helices of collagen, elastin and keratin recovered from animal by-products: functionality, bioactivity and trends of application o Research Paper on collagen from bovine bones in preparation “ Waste managements in the European Union should be improved, with the view of protecting, preserving and improving the quality of the environment, protecting human health, and ensuring prudent and rational utilisation of natural resources ” (EU 2 nd July 2014, New Directive on Waste)

Th Thank Yo You fo for yo your atte tentio tion Acknowledgmen ents ts Dr. V . Véroni onique ue-San anté é Lhout outellier (Qua QuaPA) ) and D Dr. Ma Marc A Anto ton (B (BIA)