Synthetic Unity Presented by Braxton Carter Daniel Griffin Wenjun Kong Dani Bauhan with much appreciation to Briana Harvey Starring Michael Riddle Victor the Vector Featuring Designs by Ayla Walter as Educational Model Advised by Dr. Richard Anthony Dr. Dave Goulet with contributions from
E. coli expresses Mating Factor α on the cell surface with Ice Nucleation Protein.
Yeast construct links His3 expression to the Mating Pheromone α Response Pathway.
Yeast expresses Lactate Dehydrogenase to facilitate E. coli survival with lactate.
E. coli construct links histidine expression to the lactate inducible promoter.
Synthetic unity system creates a symbiotic relationship between Yeast and E. coli . Yeast E. coli
Yeast expresses Blue Fluorescent Protein after binding of MF α with Fus1 promoter.
Victor the Vector provides better Synthetic Biology education for all students.
Victor the Vector is an electromechanical device that explores systems in ‘dry-lab.’
Victor the Vector interfaces with users through an interactive software platform.
After turning on Victor the Vector, the user selects a system to build.
The user builds the selected system on the Victor the Vector platform.
The software prompts the user about whether the system is built correctly. No Yes
The program tells the user when they build the system correctly. Video Time!
Victor the Vector is a pin-to-pin, voltage- gated circuit.
Poly-lactic acid (PLA) is a biodegradable plastic that we used for 3D printing.
Scrap PLA is hydrolyzed to create a lactic acid monomer solution.
Solution was characterized using a polarimeter to find the optical rotation. pH Optical Rotation 2.5 .12 3.5 -.13 4.21 -.26
An LDH assay was used to determine the concentration of the final solution.
Team RHIT modeled synthetic unity to educate across diverse backgrounds and spread a uniting message.
Questions? Special Thanks to: Dr. Richard Anthony Dr. Dave Goulet Dr. Alan Chiu Shannon Tieken
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