SISC 2017 Research Poster Presentation
SISC 2017 Poster Abstracts 2
SISC 2017 Bioinformatics & Computational Biology and Microbiology 3
SISC 2017 1. Poster Code : BC01 Poster Title : Relation Between Factors of Stomatal Opening and Growth in the Orchid Poster Abstract : Orchid (Phalaenopsis) is a relatively growing slowly plant. In this research, with an eye to observing the changes of orchid development and the growth trend of orchids, we added auxin, potassium chloride and carbon dioxide to control the photoperiod. By increasing the number or area of the opening orchid stomata, thereby increase the efficiency of carbon fixation and photosynthetic production, which accelerated the growth of plants and shortened the incubation time. It was found that the orchids added with auxin had better growth in all aspects. In contrast, watering with potassium chloride will limit the quantity of stomata of orchids. The environment with high concentration of carbon dioxide could make the orchid blossom faster and increased the number of stomas. Orchids with longer night period not only grow faster but the number of their stomata increases at the same time. However, there is no bloom observed. In conclusion, when extending the opening time of orchid stomata, we can increase the growth trend of orchids and accelerate the growth, which is beneficial to the development of orchids and saves cultivation time. Student Presenter : Lin Ching-Ling 4
SISC 2017 2. Poster Code : BC02 Poster Title : Regeneration of Planarian Flatworms Under Varying Environmental Conditions Poster Abstract : Regeneration refers to the regrowth of damaged tissue - a process which would have first emerged during the Permian Period, almost 300 million years ago. Wide ranges of organisms, including planarian flatworms, have continued to display this spectacular phenomenon. While humans also have the ability to regenerate, our capabilities are to a much smaller degree. For instance, if only a portion of the liver is damaged, it is able to grow the lost or scared tissue. This investigation modelled human tissue regeneration on planarian flatworms due to their genetic similarity. However, it is important to consider that while the flatworm is a suitable model, there are still certain biological discrepancies between the two organisms. The research aimed to determine which factors may act as potential blockers or enablers when regenerating human tissue by observing the flatworm's regenerative capacity under environmental conditions including varying degrees of light intensity, temperature and nourishment. The comparison was achieved by measuring the period of time they took to re-grow the severed structure thereby determining which environmental conditions yielded the most promising results of regeneration. The investigation found that flatworms subjected to minimal light exposure all provided examples of optimal tissue regrowth. The results collected by this experiment also suggest that low to room temperature are favourable conditions for a consistent regeneration process as they simulated those of the flatworm's standard environment. Concerning sustenance levels, our results indicate that the best feeding option would be to provide nutrition once a week. : Student Presenter Tikiri Silva 5
SISC 2017 3. Poster Code : BC03 Poster Title : Could Augmented Immortality Create a Digital and Deathless Version of Ourselves? Poster Abstract : Advances in artificial intelligence could provide digital immortality, distilling a lifetime’s worth of online presence into an algorithm that could resemble people’s opinions, thoughts, and interests. To answer the title question, the term “augmented eternity” will be defined and a survey will be conducted on how it will be appreciated in society. An experiment will also be undertaken, in which we look at the digital footprint that is left behind by us in a 24 hours' time span and look at how much data is produced that could be used to develop an algorithm. The ethical issues will then be discussed; such as data privacy and potential dangers that may occur with this technology. However, the benefits of this technology for mankind will also be discussed. Afterward, more research will be conducted that looks into how augmented immortality could be made possible, explaining the scientific principles; for example, how algorithms could be created from our search history, things the test subjects liked and commented on and many other things by analysing large amounts of digital data they produce every day, identifying patterns and making predictions. Looking at the Michael Jackson's Hologram Tour, which will take place this year using a form of augmented immortality will give an overview of how advanced current technology is in this field. In the end, conclusions will be drawn from the research and approximate when this technology can be included in our everyday lives. Student Presenters : Eugenia Camnahas Julia Schoetzig 6
SISC 2017 4. Poster Code : MB01 Poster Title : The Inhibition of Bacteria Growth by Organic Compounds Poster Abstract : This research aims to understand how organic compounds affect bacteria. I came up with this idea after seeing a wet towel that with a package that read “ This definitely sterilizes the bacteria on your hands with 70% of the ethanol.” I was wondered how ethanol effects bacteria. First, bacteria were gathered from my hands using deionized water and kept in a beaker. The water was divided into two parts. One of them was mixed with water and the other one was mixed with ethanol. Those liquids were then put on LB mediums and left for three days. The results showed that the ethanol one succeeded in sterilizing the bacteria and the water one failed. It was almost the same as I predicted. However, I noticed that the method for gathering bacteria was not reliable and needed to be improved. To compare the subjects clearly and accurately, the bacteria will be replaced with E. coli bacteria prepared in one petri dish. I will then split the E. coli bacteria into different samples. I am now wondering what compound in ethanol affects the bacteria. I have decided to follow up my research by experimenting with organic compounds. The next experiment will be done with some alcohols that have different number of carbon molecules. For instance, propanol, methanol, and butanol. Student Presenter : Mana Yamamoto 7
SISC 2017 5. Poster Code : MB02 Poster Title : How Does Cisplatin (chemotherapeutic drug) Affect Human DNA Poster Abstract : Cancer is the second leading cause of death globally and was responsible for 8.8 million deaths in 2015. Globally, nearly 1 in 6 deaths is due to cancer. There are different treating methods such as surgery, radiotherapy, and chemotherapy. Cisplatin is the most important and efficacious chemotherapeutic agent for the treatment of advanced cancer. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. By briefly understanding how DNA is structured, how it replicates, how cancer emerges and how it spreads, research can be started. Delving into how cisplatin works, how it destroys cancer cells and how it affects DNA and the DNA replication in that process will answer the title question. Thereupon the effectiveness of the drug by taking a look at its side effects and explaining how it works when it is combined with other chemotherapeutic drugs will be discussed. In the end, the research will be summarised, and conclusions will be drawn. Student Presenters : Rebecca Clegg Julia Schoetzig 8
SISC 2017 6. Poster Code : MB03 Poster Title : Cloning and Expression of a Full-Length Recombinant NS1 Protein from Zika Virus in Escherichia Coli Poster Abstract : The mosquito-borne Zika virus (ZIKV) is a member of the Flavivirus genus in the Flaviviridae family, which was discovered in Uganda almost 70 years ago, it was spreading widely in South and Central America, the Caribbean, The Pacific, and Vietnam. ZIKV infections are frequently asymptomatic or cause mild disease like Dengue. Recently, severe complication of ZIKV infection are reported, including central nervous system abnormalities (especially microcephaly) in foetuses/neonates and Guillain-Barre syndromes in adults. An accurate diagnosis of ZIKV infection is essential for timely management of the disease. NS1 is a about 43 kilodalton that can be detected in serum of patients with the febrile phase of dengue virus (DENV) infection. Centers for Disease Control and Prevention (CDC) recommends NS1 and Zika IgM ELISA as an excellent diagnostic approach. In this study, Zika virus was used as a template to produce DNA clones of the full-length NS1 genes via reverse transcriptase synthesis of Complementary DNA (cDNA) by Polymerase Chain Reaction (PCR) amplification of the NS1 region. Products were cloned in TA plasmid and subsequently subcloned into pET-28A (+) vector to construct a recombinant plasmid. The recombinant plasmid was transformed into E. coli BL21 strains and NS1 expressed protein was identified by SDS-PAGE and western blotting. This research need to be further investigating to develop a NS1 diagnosis test of ZIKV in Vietnam. Student Presenter : Quynh Chi Le Vu 9
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