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Protein Structure Determination An Introduction Chad A. Brautigam, - PowerPoint PPT Presentation

Nov 13, 2022 •14 likes •274 views

Protein Structure Determination An Introduction Chad A. Brautigam, Ph.D. Structural Biology Laboratory UTSW Medical Center at Dallas What is Protein Structure? Arg-Lys-Ala-Gln-Trp-Cys-His-Ala-Asp What is Protein

  1. Protein Structure Determination An Introduction Chad A. Brautigam, Ph.D. Structural Biology Laboratory UTSW Medical Center at Dallas

  2. What is “Protein Structure?” … Arg-Lys-Ala-Gln-Trp-Cys-His-Ala-Asp…

  3. What is “Protein Structure?” • It is the description of the three- dimensional architecture of a polypeptide. • Our aim: to know accurately and precisely the position of every atom in a protein. That’s usually between 2000 and 10,000 atoms!

  4. Wherefore Art Thou, Protein Structure? • To know a protein’s shape is to get a glimpse of its function.

  5. Sometimes, Structure = Function

  6. Other Times, Structure Suggests Function A structure can give us some wonderful things, i.e. testable hypotheses!

  7. How Do We Obtain Protein Structures? • Nuclear Magnetic Resonance (similar to MRI) • Electron Crystallography • Neutron Crystallography • X-ray Crystallography

  8. In Order to Do Protein Crystallography, We Need… Protein Crystals!

  9. Protein Crystals In order to obtain crystals of a protein, it is almost always necessary to have pure protein.

  10. Protein Sources • Animal, plant, or microbial tissues or cultures. • Companies. • Overexpression. – E. coli. – Insect cells. – Mammalian cell culture.

  11. Protein Purification

  12. Protein Purification • We use the physical properties of the protein of interest to separate it from all contaminants. • Chief among these properties are: – Charge. – Size.

  13. Quality Assessment PAGE

  14. Quality Assessment Mass Spectrometry

  15. Now That We Have Pure Protein… How do we crystallize it?

  16. Protein Crystallization

  17. Some Common Water- Withdrawing Chemicals (Precipitants) • Polyethylene Glycol. • Ammonium Sulfate. • Sodium Chloride. • 1,4 Methyl Pentane Diol. • Ethyl Alcohol.

  18. Enough Theory- How Do We Actually Do This? Vapor Diffusion!

  19. Vapor Diffusion “Drop” Crystal “Cover slip” “reservoir”

  20. In Most Cases, Protein Crystals Are Not an End They are the means by which we can determine a protein’s structure.

  21. X-ray Diffraction by Crystals X-ray X-ray Generator Detector

  22. An X-Ray Diffraction Pattern

  23. Electron Density

  24. Analysis of the Protein Structure

  25. Analysis of the Protein Structure

  26. Analysis of the Protein Structure

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