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Our team Lucas Alan Carlos Jlio Fernando Marianna Paola - PowerPoint PPT Presentation

Our team Lucas Alan Carlos Jlio Fernando Marianna Paola Raphael talo Clara Priscila Liza Omar Santuza Where we came from Minas Gerais, Brazil Where we came from Belo Horizonte - MG Where we came from Universidade Federal de


  1. Our team Lucas Alan Carlos Júlio Fernando Marianna Paola Raphael Ítalo Clara Priscila Liza Omar Santuza

  2. Where we came from Minas Gerais, Brazil

  3. Where we came from Belo Horizonte - MG

  4. Where we came from Universidade Federal de Minas Gerais (UFMG)

  5. A worldwide problem Acute Coronary Syndrome (ACS) NIH Medline Plus Winter 2009 Issue: Volume 4 Number 1

  6. A worldwide problem Acute Coronary Syndrome (ACS) WHO, 2013

  7. A Brazilian problem Acute Coronary Syndrome (ACS) Teich et al., Rev Bras Cardiol., 2011

  8. A challenge Acute Coronary Syndrome (ACS)

  9. Aiming high • To develop a sensitive device capable of detecting and integrating the signals of ACS biomarkers, setting a prognosis.

  10. Biomarkers

  11. TMAO: Introduction Trimethylamine-N-Oxide Tang et al., N Engl J Med., 2013

  12. TMAO: Construction Trimethylamine-N-Oxide TMAO TMAO ‘ TorT TorT TorS TorR* TorR BBa_K1086000 -------- Ba_K1086002 ------- RFP pTorCAD

  13. TMAO: Construction Trimethylamine-N-Oxide TMAO TorT + TMAO TMAO ‘ TorT TorT TorS 2 TorT + 2 TMAO + TorS TorR* TorR RFP pTorCAD

  14. TMAO: Modeling Trimethylamine-N-Oxide

  15. TMAO: Results Trimethylamine-N-Oxide TorCAD+RFP ----- TMAO Fluorescence/Absorbance

  16. IMA: Introduction Ischemia Modified Albumin Normal patient blood Ischemic patient blood (Cobalt added) (Cobalt added) Albumin IMA

  17. IMA: Construction Ischemia Modified Albumin Normal patient blood Ischemic patient blood (Cobalt added) (Cobalt added) Albumin IMA pRCNA YFP -------- Ba_K1086001 --------

  18. IMA: Modeling Ischemia Modified Albumin

  19. IMA: Modeling Ischemia Modified Albumin Albumin 90 µM

  20. IMA: Modeling Ischemia Modified Albumin Cobalt 25 µM

  21. IMA: Results Ischemia Modified Albumin RCNA+YFP ----- Cobalt Free Cobalt Assay: Fluorescence/Absorbance

  22. IMA: Results Ischemia Modified Albumin RCNA+YFP ----- Cobalt Bovine Serum Albumin Assay: Fluorescence

  23. IMA: Results Ischemia Modified Albumin RCNA+YFP ----- IMA+Cobalt Ischemic and Non-Ischemic Mice Sera Assay: Fluorescence Ischemic Non-Ischemic

  24. Human Practices Talking with specialists Neuza Antunes Rodrigues Doctor Antonio Luiz Pinho Ribeiro BIOSAFETY ETHICS

  25. Human Practices Scientific dissemination Interest over time. Web Search. Worldwide, 2004 - present. Interest over time. Web Search. Worldwide, 2004 – present English x Portuguese

  26. Human Practices Goals Talking Scientific with dissemination specialists Cardbio Impact Thinking Collaboration outside the box

  27. Human Practices Scientific dissemination Our team

  28. Human Practices Scientific dissemination Our university Our team

  29. Human Practices Scientific dissemination

  30. Human Practices Scientific dissemination Public schools Our university Our team

  31. Human Practices Scientific dissemination UFMG & Escolas

  32. Human Practices Scientific dissemination The Brickard Game Missions 1. Fuel from sunlight 2. Microplastic hazard on the oceans 3. Spoiled meat 4. Malaria and artemisin 5. Celiac disease 6. Space exploration 7. Our own project

  33. Human Practices Scientific dissemination Our state Public schools Our university Our team

  34. Human Practices Scientific dissemination

  35. Human Practices Scientific dissemination Brazil Our state Public schools Our university Our team

  36. Human Practices Scientific dissemination Website

  37. Human Practices Scientific dissemination Wikipedia facebook.com/igemufmg twitter.com/igemufmg

  38. Human Practices Scientific dissemination Newspaper Video about Synthetic Biology

  39. Human Practices Goals Talking Scientific with dissemination specialists Cardbio Impact Thinking Collaboration outside the box

  40. Human Practices Thinking outside the box Hackspace

  41. Human Practices Thinking outside the box Our song!

  42. Human Practices Goals Talking Scientific with dissemination specialists Cardbio Impact Thinking Collaboration outside the box

  43. Human Practices Collaboration

  44. Human Practices Collaboration Jamboré – Meeting of Brazilian teams

  45.  Achievements  Assembly, registry and characterization of new biobricks:  RCNA + YFP (BBa_K1086001)  TorCAD (BBa_K1086000)  TorCAD+ RFP (BBa_K1086002)  Modeling of our systems.  Expand the knowledge about synthetic biology in our University, our State and our Country.

  46. Thanks Sponsors Other supporters Laboratório de Genética Bioquímica Professor Gastone Castellani Laboratório de Sinalização Celular e Professora Andréa Mara Macedo Nanobiotecnologia Neuza Antunes Rodrigues Laboratório de Genômica e Genética de Parasitos Juliana Pimenta Laboratório de Imunoquímica de Toxinas Naturais Rosalvo Fogaça – UFPR Laboratório de Sistema de Informação Izabella Pena Neshich – UNICAMP Laboratório de Angiogênese Doutor Antonio Luiz Pinho Ribeiro André Luis Martins Reis – Johns Hopkins

  47. Thanks Crowd funding donators Fernanda Santos Freitas Denis Roger Pedro E. C. Vieira Pedro Henrique Nascimento de Aguiar Francislon Silva de Oliveira Filipe Silva Villela Lucas Antunes de Siqueira Costa Sabrina Lima Pedro Pinho Teixeira Marina Diniz Jarbas Rohr Diego Pessoa Santos Mainá Bitar Graciela Kunrath Lima Leonardo Lucas Carnevalli Dias Bel Bitar Luandy Baião Isabela F. Prates Wesley Júnio da Silva Marcela Segatto do Carmo Michele Araújo Henrique Teichmann Neuza Antunes Nicole Scherer Juliano Leal Wagner Arbex Ceres Luciana Alves Glória Franco Maíra Freire Naíla Barbosa da Costa Amanda Campelo Thank you!

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