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Nov 25, 2023 •48 likes •508 views

Nagahama About our Institute Nagahama Institute of Bio-Science and Technology Japan, Shiga, Nagahama city rice How to solve Plasmid construction-1 RBS MT Cd p Catching E.coli has two merits Cd Cd Cd Catching E. coli is

  2. Nagahama

  3. About our Institute • Nagahama Institute of Bio-Science and Technology Japan, Shiga, Nagahama city

  5. rice

  8. How to solve

  10. Plasmid construction-1 RBS MT Cd p

  11. Catching E.coli has two merits

  12. Cd² ⁺ Cd² ⁺ Cd² ⁺ Catching E. coli is not weaken or is killed by absorbed cadmium. Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺

  13. Recyclable system of cadmium Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺ Cd² ⁺

  14. We have constructed fusion protein RBS MT Cd p

  15. But we have problems

  16. E. coli may escape from cadmium by negative chemotaxis.

  17. Thanks for

  18. Modeling by UT-Tokyo

  19. Swarming assay E. coli E. coli E. coli go away from cadmium!!! Cd ddH2O

  20. If use only catching E. coli

  21. How to solve? Gathering to cadmium ion → Positive chemotaxis Positive chemotaxis factor → Aspartic acid(Asp)

  22. Go to Asp?

  23. E. coli Asp_concentration

  25. E. coli go to Asp!!!

  26. Using AspA OM IM fumafic acid aspartic acid ammonium ion RBS AspA Cd p

  27. Aspartic acid synthesis by Gathering E. coli − Cd 2+ + Cd 2+ 1 50 1 200 1 100 1 100 1 50 1

  28. Overview

  29. protease ClpXP Protease: ClpAP Digested!! AspA LVA tag

  30. Remove LVA tag AspA LVA tag BBa_C0083

  31. Policy & Practice

  32. “The 86 th Annual Meeting of The Genetics Society of Japan”

  33. We conducted a presentation Policy&Practice

  34. We did a poster session Policy&Practice

  35. We did a poster session Policy&Practice

  36. We did a poster session Policy&Practice

  37. Ｎｉｃｅ Ｓｍｉｌｅ！

  38. Acknowledgement • Instructors Assosiate Prof. Shoji Usami Prof. Yoshisuke Nishi

  39. Acknowledgement ・ Advisors Ryuhei Minei Third degree of undergraduate student

  40. Acknowledgement • Swarming assay : NIB. Plant molecular Physiology laboratory • Fusion protein : Prof. Atsushi Ohshima • Cd handling : Prof. Yasushi Kawai

  41. Acknowledgement Camera : Prof. Sanji Matsushima TLC : Assosiate Prof.Makoto Hasegawa SDS-PAGE: Assistant staff Takashi Hamada

  42. Acknowledgement Laboratory equipment : Assistant staff of Dept. Biosci. Genom ics Course Assistant staff of Dept. Biosci. Protemics Course Assistant staff of Dept. Biosci. Cell Bio Course Assistant staff of Dept. Biosci. Enviro Bio Course Assistant staff of Dept. Animal Biosci

  43. Acknowledgement • Presentation support Assosiate Prof.Kazue Asou Hayato Saigo • Modeling support UT-Tokyo

  44. Sponsors

  45. Thank you for listening of our presentation

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