21/07/54 Oral Presentation CRDC 5 LOGO LOGO Roles of silicon on growth Roles of silicon on growth of of Thai jasmine rice Thai jasmine rice ( Oryza Oryza sativa sativa L. cv. KDML L. cv. KDML105 105) ) under salt stress under salt stress P Presented by Chakkree Lekklar t d b Ch kk L kkl Advisor: Dr. Anchalee Chaidee Introduction Introduction Salt stress Salt stress • Osmotic stress • Osmotic stress - decreased water potential http://www.fao.org/DOCREP/ • Oxidative stress - reactive oxygen species reactive oxygen species • Ion toxicity - [Na + ], [Cl - ] 1
21/07/54 Oral Presentation CRDC 5 Silicon (Si) Silicon (Si) Silicon (Si) Silicon (Si) • Beneficial element (Arnon and Stout, 1939) • The difference in Si accumulation is dependent on root uptake ability. (Ma and Takahashi, 2002) • Si helps plants to overcome multiple stresses. • biotic stresses • abiotic stresses LOGO • Silicon induced disease resistance in rice. (Rodrigues et al , 2009) Symptoms of sheath blight on six Brazilian of rice. (A) Control (0 g Si pot -1 ) and (B) plants grown at the highest rate of Si(1.92g Si pot -1 ) • Silicon alleviated heavy metal toxicity. (Vaculík et al ., 2009) 2
21/07/54 Oral Presentation CRDC 5 • Silicon alleviated salt stress (Zhu et al ., 2004) Effects of Si on shoot and root dry weight (g/pot) of 10-day treatment cucumber plants grown in nutrient sol n containing Effects of Si on shoot and root dry weight (g/pot) of 10-day treatment cucumber plants grown in nutrient sol n containing 0 or 50mM NaCl 0 or 50mM NaCl -1 weight) Shoot Na concentration ( μ mol g cultivar - Si + Si Reduction (%) 408 ± 104 14.5 477 ± 216 CRS 10 866 ± 131 314 ± 147 63.7 GR 4 (Yeo et al ., 1999) 423 ± 135 328 ± 89 22.5 IR 36 Effect of Si on shoot Na content of 3 cultivar of Oryza sativa L. grown for 7 d in culture sol n salinized with NaCl (50 mM) Effect of Si on shoot Na content of 3 cultivar of Oryza sativa L. grown for 7 d in culture sol n salinized with NaCl (50 mM) Thai jasmine rice Thai jasmine rice • Khao Dok Mali 105 (KDML 105) http://negistda.kku.ac.th/research/research09 / LOGO http://www.aftc.or.th/itc/products_analyze_price_01.php?id=56andfgrp_id=5andfmnu_id=18 3
21/07/54 Oral Presentation CRDC 5 Objective Objective To investigate roles of silicon on growth of To investigate roles of silicon on growth of Thai jasmine rice under salt stress Thai jasmine rice under salt stress Thai jasmine rice under salt stress Thai jasmine rice under salt stress http://johnseconblog.files.wordpress.com http://www.nakorntonrice.com/images LOGO Materials and Methods Materials and Methods 3-week-old seedlings Salt stress & Si treatments -WP (Control) -WP + 0.5 mM Si -NaCl (60 mM) -NaCl + 0 5 mM Si NaCl + 0.5 mM Si growth measurement (at 7 and 14 days) LOGO 4
21/07/54 Oral Presentation CRDC 5 • Determination of photosynthetic pigments � Chlorophyll a � Chlorophyll b � Carotenoids � C d (Arnon, 1949) LOGO Results Results Results Results and and Discussion Discussion LOGO 5
21/07/54 Oral Presentation CRDC 5 Shoot fresh weight 1.335 0.745 0.752 0.630 0.553 0.355 Day after treatment LOGO Root fresh weight 0.295 0.168 0.158 0.150 0.129 0.083 Day after treatment LOGO 6
21/07/54 Oral Presentation CRDC 5 Shoot dry weight 0.187 0.142 0.110 0.105 0.095 0.064 Day after treatment LOGO Root dry weight 0.029 0.016 0.016 0.015 0.014 0.008 Day after treatment LOGO 7
21/07/54 Oral Presentation CRDC 5 Discussion Discussion • Salt stress inhibited mass production of KDML KDML105 rice. • Relative water contents (RWC) did not change by salt stress. LOGO Discussion Discussion • Si completely eliminated effect of salt stress on growth during 7 days. • In prolonged salt stress (14 days), Si In prolonged salt stress (14 days), Si enhanced growth of shoot, but not root. LOGO 8
21/07/54 Oral Presentation CRDC 5 Root/shoot ratio • In KDML105 rice, salt stress decreased root/shoot ratio decreased root/shoot ratio • In general, salt stress increases root/shoot ratio increases root/shoot ratio Day after treatment LOGO Chlorophyll a contents 3.18 2.78 1.64 Day after treatment LOGO 9
21/07/54 Oral Presentation CRDC 5 Chlorophyll b contents 1.09 0.96 0.56 Day after treatment LOGO Carotenoid contents 1.15 0.9 0.53 Day after treatment LOGO 10
21/07/54 Oral Presentation CRDC 5 Discussion Discussion Pepper grown under drought stress (Lobato et al ., 2009) Chlorophyll a (a) and Chlorophyll b (b) in Capsicum annuum cv. Vermelho Gigante under the treatments control, 0.25 μ M Si, 1.00 μ M Si, 1.75 μ M Si and stress. Discussion Discussion Effects of Si on chlorophyll a contents (A), chlorophyll b contents (B), carotenoid contents (C), total chlorophyll contents (D), chlorophyll a / b in cucumber leaves under Cd stress. LOGO (Feng et al , 2010) 11
21/07/54 Oral Presentation CRDC 5 Conclusion Conclusion • Si alleviates salt stress by maintaining photosynthetic pigment and shoot growth of KDML 105 rice. LOGO Further works Further works • To examine effect of salt stress on induction of oxidative stress and antioxidant systems of oxidative stress and antioxidant systems • To test whether Si-enhanced growth is associated with the activation of antioxidant systems under salt stress LOGO 12
21/07/54 Oral Presentation CRDC 5 Acknowledgements Acknowledgements • Higher Education Research Promotion and National Research University Project of Thailand , Office of the Higher Education University Project of Thailand , Office of the Higher Education Commission • Research Program on Conservation and Utilization of Biodiversity and the Center of Excellence in Biodiversity, Faculty of Science, Chulalongkorn University • Environment and Plant Physiology Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University LOGO LOGO LOGO Thank you h k for your attention! attention! 13
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