Grafton New Zealand Ltd. Contact us : webmaster@grafton-nz.com Grafton New Zealand Ltd.
What is Equixanthin TM ? ・ Equixanthin TM is produced by a natural (non-GMO) soil microbe using highly controlled cGMP fermentation and DSP facilities. ・ Equixanthin TM is a new, all natural source of Astaxanthin in the non-esterified form with 3S,3’S I isomer. ・ It is differentiated from other sources by being a natural blend of a number of antioxidant xanthophylls. ・ In addition to Astaxanthin, Equixanthin TM also contains Adonirubin and Adonixanthin, xanthophylls with 2-3 times the antioxidant power of Astaxanthin. ・ Equixanthin TM is stable and safe. Passed safety study for feed additives. < 3% loss after 1 month at room temperature(25 ℃ ). ・ Equixanthin TM improves many health conditions in horses: primarily azoturia/tying-up, susceptibility to ulcers, skin diseases, fertility issues in stallions and mares and throat infections in sale yearlings. Grafton New Zealand Ltd. 2
Equixanthin TM Production Overview Paracoccus carotinifaciens (natural soil microbe) Fermentation Grinding 0.2μm Concentration Mixing Drying Equixanthin TM Pasteurization Test assay Special mention: Ethoxyquin free, Non-feed & food additive, Non-GMO, RAC free, Dioxins free Grafton New Zealand Ltd. 3
Biosynthetic Pathway of Carotenoids Paracoccus carotinifaciens (natural soil microbe) Grafton New Zealand Ltd. 4
Composition of Equixanthin TM TYPICAL TYPICAL ITEM ITEM CONTENT CONTENT Carotenoids Inorganic Component, etc. Astaxanthin 21.7 Na 5.4 g/kg g/kg Adonirubin 5.3 P 6.7 g/kg g/kg Adonixanthin 2.9 Fe 1.9 g/kg g/kg Other Carotenoids 4.1 Ca 3.4 g/kg g/kg Total Carotenoids 34.0 K 2.5 g/kg g/kg Main Components Mg 2.7 g/kg Crude Protein g/kg 596 Lead ppm ND (QL: 0.1ppm) Crude Fat 37 Arsenic g/kg ppm ND (QL: 0.1ppm) Crude Ash 43 Mercury g/kg ppm ND (QL: 0.1ppm) Carbohydrate 285 Salmonella Negative g/kg 25g ND: Not detected, QL: Quantification Limit Grafton New Zealand Ltd. 5
Safety Study of Equixanthin TM ・ Bacterial Reverse Mutation Test (Ames test) ☞ Equixanthin TM is considered to be non-mutagenic. ・ Chromosome aberration Test (Human lymphocytes in vitro ) ☞ Equixanthin TM is considered to be non-mutagenic. ・ Micronucleus Test (Rats) ☞ Equixanthin TM dose not induce micronuclei in bone marrow cells. ・ Acute Oral Toxicity Test (Rats) ☞ The acute oral median lethal dose (LD 50 ) of Equixanthin TM is higher than 5000 mg/kg. ・ 90-Day Toxicity Study (Rats) ☞ No observed adverse effect level (NOAEL) of Equixanthin TM is higher than 3000 mg/kg/day. ・ Acute Inhalation Toxicity study (Rats) ☞ The acute inhalation median lethal concentration (4hr LC 50 ) of Equixanthin TM is greater than 4.92 mg/L. Special mention: All studies were conducted in compliance with GLP. Grafton New Zealand Ltd. 6
Preservation Stability of Equixanthin TM Conditions: 25 ℃ , 20-45% R.H. Conditions: 40 ℃ , 75% R.H. Packaging: Usual seal in aluminum bag Packaging: Usual seal in aluminum bag Degradation of Astaxanthin (Average SD) Degradation of Astaxanthin (Average SD) Astaxanthin remaining (%) Astaxanthin remaining (%) 105 105 100 100 95 95 90 90 85 85 0 1 3 6 12 18 24 30 36 0 1 3 6 12 Storage period (months) Storage period (months) Conditions: 25 ℃ , 20-45% R.H. Condition: 60 ℃ Packaging: Open aluminum bag in the dark Packaging: Usual seal in aluminum bag Degradation of Astaxanthin Degradation of Astaxanthin Astaxanthin remaining (%) Astaxanthin remaining (%) 105 105 100 100 95 95 90 90 85 85 0 1 2 3 0 1 2 3 Storage period (months) Storage period (months) Grafton New Zealand Ltd. 7
Antioxidant Action of Astaxanthin 1 st action: Elimination of Singlet Oxygen Astaxanthin O 2 1 O 2 O 2 Singlet Oxygen Oxygen Oxygen Lipid Superoxide Hydrogen Hydroxyl Oxygen anion radical peroxide radical Lipid Lipid Lipid Lipid Radical peroxyl radical peroxide autoxidation Astaxanthin 2 nd action: Inhibition of Lipid Peroxidation Grafton New Zealand Ltd. 8
Utility in Clinical Practice by Astaxanthin １． Antioxidant action ・ muscle fatigue resistance ・ prevention of tying-up syndrome ・ improvement of conception rate ・ adjustment of estrous cycle & heat-stress relief ・ enhancement of immune system ・ anti-inflammatory & anti-photoaging effect ・ prevention of metabolic syndrome, etc. ２． Unknown action Do not drop the body weight ! ・ improvement of intestinal flora ? + High-fat diet High-fat diet) 0.02 % Astaxanthin It’s extremely important for Astaxanthin effect health maintenance. Abnormal intestinal flora Normal intestinal flora 【 Authority 】 Effects of Astaxanthin on Intestinal Micro flora in Mice Fed a High-fat Diet. Anti-aging medicine. 10 (4), 77-91, 2013 Grafton New Zealand Ltd. 9
Administration of Equixanthin TM Temporal change in Astaxanthin (Average SD) Test methods Astaxanthin concentration in horse serum (µg/L) 1. Horses 1.0 Administration period: 14 day ・ 8 thoroughbreds (breeding mares) 0.9 ・ age: 3 ～ 14 years 0.8 ・ body weight: 651 ± 35 kg 0.7 2. Dose ・ 50 mg astaxanthin ※ × 2 times /day 0.6 ・ total daily dose: Ax 100 mg / head 0.5 ※ Equixanthin TM (Grafton NZ Ltd.) 0.4 3. Administration Period 0.3 ・ 14 day continuous- administration 0.2 4. Blood Sampling Point 0.1 ・ 1, 3, 7, 14, 21, 28 day 0.0 1 3 7 5. Analysis of Astaxanthin in serum 14 21 0 4 8 12 16 20 24 28 ・ HPLC Time after administration (Day) ・ Silica gel, Acetone: Hexane (2:8) Blood concentration of astaxanthin achieves a stable state from day 7 after the start of the administration. Grafton New Zealand Ltd. 10
Anti-oxidative Activity of Adonirubin and Adonixanthin (1) Elimination of Singlet Oxygen ( 1 O 2 ) Adonirubin and Adonixanthin react more efficiently with 1 O 2 than astaxanthin. Carotenoid Relative activity (3S,3’S)-Astaxanthin Astaxanthin 1 ☞ Adonirubin 2.3 (3S)-Adonirubin Adonixanthin 2.9 (3S,3’R)-Adonixanthin Quenching activity of 1 O 2 in aqueous solution (Average SD) Quenching activity of 1 O 2 produced by a hematoporphyrin-UVA system in 4.0 aqueous solution by carotenoids. The reaction mixtures consisted of 62.5 ESR signal intensity μM hematoporphyrin and 50 mM TMPD dissolved in 50 mM phosphate 3.0 buffer, and 625 μM astaxanthin, adonirubin, or adonixanthin dissolved in * acetone at 22 ℃ in a total volume of 0.2 mL of 50 mM phosphate buffer 3.20 2.0 (pH 7.5). Results of ESR spin-trapping experiments are expressed as the 2.35 mean ± SD of three repeated experiments. Statistical analysis was ** ** 1.0 performed by analysis of variance (ANOVA) followed by Post-hoc test with 1.01 a 1 % or 5 % significance of difference. There were significant differences 0.81 0.0 (* P < 0.05 , ** P < 0.01 ) in the 1 O 2 quenching activities of carotenoid Control Astaxanthin Adonirubin Adonixanthin treatment groups compared with the control group. (2) Inhibition of Lipid Peroxidation Adonirubin and Adonixanthin show slightly weaker activity than astaxanthin. 【 Authority 】 Anti-oxidative, anti-tumor-promoting, and anti-carcinogenic activities of adonirubin and adonixanthin. J. Oleo Sci. 62 (3), 181-186, 2013 Grafton New Zealand Ltd. 11
Natural Astaxanthin from Paracoccus carotinifaciens Imported by: Grafton New Zealand Ltd contact us: webmaster@grafton-nz.com Products available from: Grafton New Zealand Ltd. contact us: webmaster@grafton-nz.com Products available in Australia from: Beamish Bloodstock Pty. Ltd. contact: beama888@gmail.com Grafton New Zealand Ltd.
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