FACSymphony Platforms for flexible research. Geoffrey Osborne - PowerPoint PPT Presentation

FACSymphony Platforms for flexible research. Geoffrey Osborne Director, Special Order Research Products For internal use only 1 Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ instruments For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ instruments Overarching goal to build flexible high performance systems. Deeply imbedded in our philosophy is the drive to help our customers answer their scientific questions. Seek to do this at multiple levels: Lasers • Detectors • Fluorophores • Software • For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ instruments To that end we have been developing systems with high numbers of lasers with variable laser power options. Deep UV Laser IR Detectors For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Flexibility presents challenges! Overarching goal to build flexible high performance systems. Deeply embedded in our philosophy is the drive to help our customers answer their scientific questions. Seek to do this at multiple levels: Lasers (power variability) • Detectors (sensitivity variability) • Fluorophores • Software • For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Flexibility presents opportunities! High Parameter Applications Support: Two types of beads for system characterization are currently in development: Those that aid in the assessment of Q and B. • Those that aid in the assessment of system function. • For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

New beads in development OPV as operational voltages. (based on based on voltage titration of CD4 stained cells, then balanced to minimize spillover) For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ Data Acquisition and Analysis challenge. FACSChorus for FACSymphony software, currently in beta test, allows the acquisition of high parameter data past current FACSDiva limits. For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ Data Acquisition and Analysis challenge. FACSChorus for FACSymphony software acquired data showing histograms for 95 parameters. That doesn’t work for me! For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FACSymphony ™ Analysis NK Treg CD4+ T tSNE-Y CD8+ T B cells pDCs mDCs Monocytes tSNE-X For internal use only FlowJo, LLC: Timothy Crawford, Ph.D. & Mike Stadnisky, Ph.D. Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Take home message. BD wants to work with you in a collegial enterprise that benefits everyone involved. We recognize that there are challenges, but those are also opportunities to do things better. For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Custom Reagents Access Robbie Lunt Marketing Product Manager, High Parameter For internal use only For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Access to “Prototype” Reagents BDB_HPS_VIP@BD.com Cumulative Customs in Inventory Current Custom Conjugation (a.k.a. Custom Reserve) 300 6-8 weeks 200 100 0 Oct Nov Dec Jan Feb Mar Apr May 2016 2017 Legacy BVs BUVs Prototype OptiBuild Style Conjugation Antibodies 2-3 weeks 1750+ Inventory Reagent For internal use only OptiBuild Reagent Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

High Parameter Reagents Brent Gaylord Director R&D, Life Science Research Reagents For internal use only For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

High Parameter Color Evolution New laser core: UUV Expanded detection Upgraded laser cores window: Hardware NIR Upgrades Filling gaps For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Roadmap Considerations BB755 New Release Access OptiBuild Process (2-3 weeks) Customs (6-8 weeks) BV Tandems BB Tandems For internal use only BUV395 & BB700 BUV Tandems Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

BD Dye Landscape DUV Base (in dev) • Deep UV DUV Tandems (in dev) • FVS440 • BUV615-P • UV BV480 • Violet BV750 • BB Tandems • BB555-P (in dev) Blue • BB755-P (new) • BYG584-P • Yellow- BYG Tandems (in dev) • Green Red For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Recently Launched: BB755-P 7 th color option for the Blue Laser • Fills the gap between: • BB700 and BB790-P1 – Performance comparable to other • Brilliant Blue Tandems CD8 – BB700 Spillover into Blue Yel-Grn Red CD4 – BB755 BB BB BB BB BYG BYG Red- APC- Red- 630 660 700 790 750 780 750 H7 805 7% 5% 20% 47% BB750-P 2% 2% 4% 2% 2% UV Violet BUV BUV BV BV BV BV BV BV 737 805 570 605 650 711 750 786 For internal use only BB750-P 30% 5% 13% 6% 5% 16% 53% 28% Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

BSB Plus Decrease of +MFI with dilution 120% 100% With the addition of reagents 80% the increase of total volume % of Max 60% can start to affect the overall CD4 40% staining with some antibodies CD19 20% 0% 0 5 10 20 40 60 80 100 120 140 160 180 200 ul wash buffer BSB+ is as affective as BSB with lower staining volume! Fresh stain 1 hr cocktail 1 hr cocktail BSB 1 hr cocktail BSB+ BV-BUV DDI For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

BD Dye Landscape DUV Base (in dev) • Deep UV DUV Tandems (in dev) • FVS440 • BUV615-P • UV BV480 • Violet BV750 • BB Tandems • BB555-P (in dev) Blue • BB755-P (new) • BYG584-P • Yellow- BYG Tandems (in dev) • Green Red For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

UV Laser FVS440 BUV615-P FVS440 BUV615-P CD4 For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

BUV615-P 7 th dye choice for UV Laser • Fills gap between • – BUV563 and BUV661 Bright reagent • Manageable SO • BUV615-P CD4 Spillover into BV BV BV BUV BUV BUV BUV BUV BUV BUV BYV BYG BYG BYG 605 650 711 395 430 563 615 661 737 805 594 615 660 710 CD4-BUV615 5% 4% 3% 5% 2% 18% 32% 14% 5% 5% 41% 13% 6% For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

FVS440 Fixable viability dye for the UV laser • Fills gap between BUV395 and BUV496 (open channel) • Compatible with common fix/perm systems • Cells may be stained, fixed, frozen, and thawed without loss of viability information • For internal use only Jurkat cells treated with or without 5 µM camptothecin for 16 hours Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group

Violet Laser BV480 BV750 BV480 BV510 CD4 (SK3) BV750 CD4 (SK3) BV480 For internal use only Information for mysymphony user group only, do not share outside the group Information for mysymphony user group only, do not share outside the group