Effects of mycorrhizal inoculation, rock phosphate and composted - PowerPoint PPT Presentation

Effects of mycorrhizal inoculation, rock phosphate and composted manure in pulse-flax rotations Dr. Yunliang Li Dr. Chantal Hamel 2018-04-10

Background ➢ Arbuscular mycorrhizal fungi (AMF) assist plants to absorb phosphorus (P) and other elements from soil ➢ In organic grain farming systems of the Prairie, soil phosphorus is often low ➢ Inoculation with AMF had increased lentil production by 30% on an organic farm

Hypotheses ➢ Finely gound rock phosphate ammended with humic acid can increase the level of available soil P and plant production ➢ Composted manure can increase the level of available soil P and plant production ➢ The arbuscular mycorrhizal fungi (AMF) help plants extract phosphorus from the soil

Experiment Design Site 2 Site 1 Factor 1 Factor 2 Swift Current Beaverlodge None 75% Rock phosphate Control Lentil Pea 150% Rock phosphate 2015 Inoculant 75% Composted Manure 150% Composted Manure Cont-Cont Cont-Inoc 2016 The carry-over effects of Flax Flax Inoc-Cont fertilization in 2015 Inoc-Inoc

Part1 ： Effects of AMF inoculation and two certified P sources (rock phosphate and composted manure) on agronomic traits and soil properties

Swift Current, 2015, Lentil Soil_N Root Yield (kg/ha) Biomass (kg/ha) Grain_N (%) Grain_P (%) Straw_N (%) Straw_P (%) (kg/ha) Soil_P (kg/ha) Colonization (%) 1134±85 Control 4053 ± 24 3.88±0.02 0.32±0.00 1.25±0.07 0.09±0.00 9.35±0.21 8.34±0.49 73.03±1.31 Inoculant 370 ± 24 1100 ± 85 3.88±0.02 0.32±0.00 1.31±0.07 0.1±0.00 8.98±0.21 8.14±0.49 76.48±1.31 0.5285 P -value 0.0858 0.9546 0.9615 0.3193 0.2903 0.0545 0.6118 0.0673 None 403 ± 30 a 1061 ± 97 3.86±0.03 ab 0.31±0.01 b 1.2±0.08 b 0.09±0.01 bc 9.47±0.27 7.01±0.59 c 74±2.07 75% RP 421 ± 30 a 1045 ± 97 3.88±0.03 ab 0.31±0.01 b 1.09±0.08 b 0.08±0.01 c 9.04±0.27 7.62±0.59 bc 72.31±2.07 150% RP 417 ± 30 a 1131 ± 97 3.85±0.03 b 0.32±0.01 ab 1.19±0.08 b 0.09±0.01 bc 8.91±0.27 7.47±0.59 bc 75.88±2.07 75% CM 388 ± 30 ab 1213 ± 97 3.85±0.03 b 0.32±0.01 ab 1.32±0.08 b 0.1±0.01 b 9.35±0.27 8.98±0.59 ab 74.75±2.07 150% CM 310 ± 30 b 1136 ± 97 3.95±0.03 a 0.34±0.01 a 1.61±0.08 a 0.13±0.01 a 9.03±0.27 10.12±0.59 a 76.81±2.07 0.3113 P-value 0.0044 0.023 0.0089 <.0001 <.0001 0.2813 <.0001 0.5956 • Introduced AMF had no effect on agronomic traits and soil properties • Composted manure at 150% recommended P level reduced lentil yield, but increased N, P level in grain and straw, and soil test P level. • Rock phosphate had no effect Note: The interaction effect of inoculation and fertilization was not significant

Beaverlodge, 2015, Pea Grain_N Straw_P Soil_N Root Yield (kg/ha) Biomass (kg/ha) (%) Grain_P (%) Grain_K (%) Grain_C (%) Straw_N (%) (%) Straw_K (%) Straw_C (%) (kg/ha) Soil_P (kg/ha) Colonization (%) Control 4267 ± 219 6324 ± 355 3.27±0.06 0.26±0.01 a 0.85±0.01 41.01±0.05 0.65±0.02 0.04±0.00 1.51±0.09 40.92±0.08 22.73±1.87 30.35±2.51 52.43±1.68 Inoculant 4394 ± 219 6424 ± 355 3.35±0.06 0.25±0.01 b 0.86±0.01 41.1±0.05 0.67±0.02 0.05±0.00 1.54±0.09 40.97±0.08 23.72±1.87 27.95±2.51 54.5±1.68 P-value 0.3305 0.6627 0.1288 0.0344 0.5571 0.1741 0.1741 0.0623 0.5955 0.7115 0.2365 0.1405 0.4752 None 4218 ± 243 a 6251 ± 406 ab 3.28±0.07 0.26±0.01 a 0.86±0.02 40.99±0.08 0.65±0.03 0.04±0.00 1.47±0.1 bc 40.83±0.13 24.32±2 31.52±2.87 ab 56.5±2.65 75% RP 4246 ± 243 a 6019 ± 406 b 3.34±0.07 0.25±0.01 ab 0.87±0.02 41.07±0.08 0.66±0.03 0.04±0.00 1.36±0.1 c 41.02±0.13 22.54±2 24.19±2.87 c 49.56±2.65 150% RP 4217 ± 246 a 6056 ± 406 b 3.24±0.07 0.26±0.01 ab 0.83±0.02 41.16±0.08 0.66±0.03 0.04±0.00 1.44±0.1 bc 41.14±0.13 21.32±2 26.44±2.87 bc 55.13±2.65 75% CM 4235 ± 243 a 6317 ± 406 ab 3.3±0.07 0.24±0.01 b 0.86±0.02 41.05±0.08 0.67±0.03 0.04±0.00 1.59±0.1 ab 40.97±0.13 24.35±2 29.53±2.87 abc 56.44±2.65 150% CM 4737 ± 243 a 7226 ± 406 a 3.4±0.07 0.25±0.01 ab 0.86±0.02 41.01±0.08 0.67±0.03 0.04±0.00 1.78±0.1 a 40.76±0.13 23.6±2 34.07±2.87 a 49.69±2.65 P-value 0.0396 0.0082 0.332 0.0232 0.681 0.6242 0.6242 0.7075 <.0001 0.248 0.1124 0.0018 0.1397 • Inoculation with AMF reduced % P grain • Composted manure increased soil mineral P content at harvest, and pea biomass, straw % K and yield, but the low rate reduced % P grain • Rock phosphate reduced soil test P Note: No interaction of inoculation and fertilization is significant, except for on mycorrhizal colonization ( p =0.0485)

Swift Current, 2016, Flax Aims: 1. Test the carry-over effect vs continuous effect of inoculation with AMF 2. The carry-over effect of composted manure and rock phosphate on crop and soil mineral N and P levels Root Colonization Yield (kg/ha) Biomass (kg/ha) plant_N (%) plant_P (%) Plant_K (%) Plant_C (%) Grain_N (%) Grain_P (%) Straw_N (%) Straw_P (%) Soil_N (kg/ha) Soil_P (kg/ha) (%) Cont-Cont 464±52.92 1696±157.17 0.73±0.03 0.18±0.01 1.48±0.07 45.08±0.12 1.98±0.02 0.6±0.01 0.27±0.03 0.09±0.01 7.99±1.33 9.43±0.93 61.6±2.83 Cont-Inoc 432±52.92 1705±157.17 0.69±0.03 0.16±0.01 1.37±0.07 45.17±0.12 2.02±0.02 0.59±0.01 0.3±0.03 0.1±0.01 8.57±1.33 7.79±0.93 61.95±2.83 Inoc-Cont 426±52.92 1537±157.17 0.7±0.03 0.17±0.01 1.45±0.07 44.89±0.12 2±0.02 0.6±0.01 0.28±0.03 0.09±0.01 8.27±1.33 9.47±0.93 62.25±2.83 Inoc-Inoc 467±52.92 1669±157.17 0.67±0.03 0.17±0.01 1.34±0.07 45±0.12 1.98±0.02 0.6±0.01 0.26±0.03 0.09±0.01 7.4±1.33 9.83±0.93 60.2±2.83 P-value 0.8059 0.7601 0.5455 0.37 0.2455 0.2201 0.416 0.8721 0.7462 0.9794 0.2699 0.0752 0.8735 None 505±56.02 1822±169.01 0.73±0.04 0.17±0.01 1.45±0.08 44.98±0.13 2.01±0.03 0.56±0.01 b 0.28±0.03 0.08±0.01 8.53±1.35 9.26±0.98 b 59.94±2.98 75% RP 440±56.02 1627±169.01 0.71±0.04 0.17±0.01 1.38±0.08 45.08±0.13 2±0.03 0.59±0.01 ab 0.28±0.03 0.08±0.01 7.6±1.35 6.74±0.98 b 59.06±2.98 150% RP 434±56.02 1693±169.01 0.74±0.04 0.19±0.01 1.51±0.08 45.04±0.13 2±0.03 0.6±0.01 ab 0.29±0.03 0.1±0.01 7.43±1.35 8.61±0.98 b 61.19±2.98 75% CM 457±56.02 1619±169.01 0.65±0.04 0.16±0.01 1.38±0.08 45.07±0.13 1.97±0.03 0.61±0.01 ab 0.26±0.03 0.09±0.01 8.42±1.35 9.03±0.98 b 63.06±2.98 150% CM 400±56.02 1499±169.01 0.66±0.04 0.17±0.01 1.32±0.08 45.02±0.13 1.99±0.03 0.62±0.01 a 0.28±0.03 0.11±0.01 8.3±1.35 12.02±0.98 a 64.25±2.98 P-value 0.4812 0.5812 0.3606 0.1137 0.3901 0.9629 0.8811 0.0093 0.7778 0.5426 0.5037 <. 0001 0.3922 • No significant effect of any inoculation treatment • The carry-over effect of compost manure at 150% recommended P significantly increased grain P level of flax, and soil P level • Rock phosphate had no effect