EC Blue for Water Testing EC Blue for water testing Can you detect - PDF document

EC Blue for Water Testing

EC Blue for water testing Can you detect contaminant (bacteria) from water without color?

EC Blue for water testing (1) Product profile, EC Blue • EC Blue was introduced in 1998, and being an official water test method in Japan approved by JWWA (2001). • There are 3 different presentations (packages) prepared for E. coli and coliform detection from water. EC Blue is “Culture Medium”. - For MPN : EC Blue 10 (in 10mL non-self fluorescent tube) and new MPN device - For detection: EC Blue 100 (in 100mL non-self fluorescent bottle) - For detection: EC Blue 100P (in a sachet for 100mL) EC Blue 10 EC Blue 100 EC Blue 100P

EC Blue for water testing (2) Medium composition of EC Blue (17.4g for 1 L) Base medium Peptone 5.0 g Sodium Chloride 5.0 g Monopotassium Phosphate 1.0 g Dipotassium Hydrogen Phosphate 4.0 g Sodium Lauryl Sulfate 0.1 g Sodium Pyruvate 1.0 g Potassium Nitrate 1.0 g IPTG (Isopropyl-beta-D(-)-Thiogalactopyranoside) 0.1 g Enzyme substrate X-GAL 0.1 g 4MUG 0.1 g

EC Blue for water testing (3) EC Blue: Feature and benefit Feature Benefit Easy to dispose after autoclaving (bottle Conservation of environment (APL does and tube are made from APL). not produce dioxin and other toxic gases). Long shelf life (1 year after manufacturing) Save inventory and purchasing cost Shorter incubation and detection time Easy and convenient to control lab compared with other products (18-24hours). workflow. Easy to judge and detect E. coli by Confirmation or re-test are not necessary fluorescence (APL is non-self fluorescent and it could be save those costs. material). Can be stored at room temperature Do not need to wait till medium to be room (under 30 C ˚ ) temperature (save preparation time).

EC Blue for water testing (4) Interpretation Coliform Positive E. coli Positive Color change of medium (Green- Observation of fluorescence Blue) might be Coliform positive might be E. coli positive

EC Blue for water testing (5) Product Comparison vs. Colilert (IDEXX) Product / manufacturer EC Blue Colilert Nissui Pharmaceutical IDEXX Comparison item Composition of the product Ammonium sulfate, other Peptone, other (Enzyme substrate used) ONPG / MUG X-GAL / MUG Fluorescent confirmation Difficult to confirm Easy to confirm (from Yellow background) from Green – Blue background E. coli, coliform only Wider than Colilert (E. coli, coliform, other contaminant Recovery bacteria) Room temp (under 30 C ˚ ) Storage condition Cool temp Package (for MPN) Glass tube APL tube (easy to dispose)

EC Blue for water testing (6) • Market situation and the future possibility of EC Blue: 1. Market situation: - Market value (US$): Europe – 10mil. / US – 9mil. / Japan – 4mil. - Competitors: less competition. IDEXX and Merck are the major players. IDEXX (Colilert) has over 80% of market share world wide. - IDEXX has many official approvals for Colilert. 2. Future possibility of EC Blue: - 100mL presentation has introduced in December 2003 (100P). - EC Blue also will apply for official approvals (if necessary, EPA/APHA/FDA/AOAC etc.). - new innovative MPN device.

EC Blue for water testing • Products Package Code EC Blue 100P EC Blue 100P 100 pcs. 05591 EC Blue 100 EC Blue 100 80 bottles 05593 EC Blue 10 EC Blue 10 150 tubes 05613 (Shelf life / Storage = 1 year / Dark and RT) EC Blue Comparator 1 bottle 05617