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Energy Efficient Wastewater Treatment Emily Gonthier & Jennifer Lawrence April 30, 2018 Outline ReNUWIt What is it? Our Partnership through ReNUWIt Research Municipal Wastewater Treatment 101 Anammox Broader


  1. Energy Efficient Wastewater Treatment Emily Gonthier & Jennifer Lawrence April 30, 2018

  2. Outline • ReNUWIt • What is it? • Our Partnership through ReNUWIt • Research • Municipal Wastewater Treatment 101 • Anammox • Broader Impacts 1

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  4. ReNUWIt – REU Program Summer 2015 – Emily joins the lab group as an REU, with Jennifer as her mentor 3

  5. ReNUWIt – REU Program Summer 2015 – Emily joins the lab group as an REU, with Jennifer as her mentor Fall 2016 – Emily joins the lab group as a PhD student! 3

  6. Municipal Wastewater Treatment 101 4

  7. Municipal Wastewater Treatment 101 4

  8. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement 5

  9. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L 5

  10. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L Oxygen- demanding BOD 100 – 350 mg/L 30 mg/L substances 5

  11. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L Oxygen- demanding BOD 100 – 350 mg/L 30 mg/L substances 10 5 – 10 8 Pathogens Fecal Coliform 500 CFU/mL CFU/mL 5

  12. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L Oxygen- demanding BOD 100 – 350 mg/L 30 mg/L substances 10 5 – 10 8 Pathogens Fecal Coliform 500 CFU/mL CFU/mL Nitrogen 30 – 50 mg/L varies Nutrients Phosphorus 20 – 40 mg/L varies 5

  13. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L Oxygen- demanding BOD 100 – 350 mg/L 30 mg/L substances 10 5 – 10 8 Pathogens Fecal Coliform 500 CFU/mL CFU/mL Nitrogen 30 – 50 mg/L varies Nutrients Phosphorus 20 – 40 mg/L varies Additional - - - chemicals 5

  14. Municipal Wastewater Treatment 101 Raw Sewage Characteristics Influent Treatment Pollutant Parameter Concentration Requirement Solids TSS 100 – 350 mg/L 30 mg/L Oxygen- demanding BOD 100 – 350 mg/L 30 mg/L substances 10 5 – 10 8 Pathogens Fecal Coliform 500 CFU/mL CFU/mL Nitrogen 30 – 50 mg/L varies Nutrients Phosphorus 20 – 40 mg/L varies Additional - - - chemicals 5

  15. Nitrogen Removal Technologies 101 6

  16. Nitrogen Removal Technologies 101 7

  17. Nitrogen Removal Technologies 101 Conventional Nitrogen Removal • Nitrification • Denitrification 7

  18. Nitrogen Removal Technologies 101 Conventional Nitrogen Removal • Nitrification • Denitrification Anammox / Deammonification • Partial nitritation • Anaerobic ammonium oxidation (anammox) 7

  19. The Anammox Process Strengths Decreased aeration demands • 60% reduction in energy consumption Decreased organic carbon demands • 90% reduction in waste biomass • Reduction in CO 2 emissions Reduction in N 2 O emissions 8

  20. The Anammox Process Weaknesses Strengths Decreased aeration demands Slow growth rate • • 60% reduction in energy consumption Long start-up periods Sensitivity to reactor conditions Decreased organic carbon demands • Instability • 90% reduction in waste biomass • Periodic Failures • Reduction in CO 2 emissions Reduction in N 2 O emissions Bacteria not yet isolated 8

  21. The Anammox Process Weaknesses Strengths Decreased aeration demands Slow growth rate • • 60% reduction in energy consumption Long start-up periods Sensitivity to reactor conditions Decreased organic carbon demands • Instability • 90% reduction in waste biomass • Periodic Failures • Reduction in CO 2 emissions Reduction in N 2 O emissions Bacteria not yet isolated Research Goal: Utilize insights from microbiology to understand and improve the functionality of the anammox process 8

  22. Laboratory-Scale Anammox Bioreactor Operating Conditions + NH 4 N 2 • Influent: - - NO 2 NO 3 • Synthetic wastewater - HCO 3 biomass • ArCO 2 • Reactor: • Volume: 1L • Temperature: 37 ° C • HRT: 12-48 hours • SRT: 50 days 1L Membrane Bioreactor 9

  23. Laboratory-Scale Anammox Bioreactor Operating Conditions • Influent: • Synthetic wastewater • ArCO 2 • Reactor: • Volume: 1L • Temperature: 37 ° C • HRT: 12-48 hours • SRT: 50 days 9

  24. Bioreactor Performance 2.5 Nitrogen Removal Rate (NRR) (g N/L·d) 2.0 NRR 1.5 1.0 0.5 0.0 0 50 100 150 200 250 300 350 400 450 500 Time (days) 10

  25. Bioreactor Performance 2.5 Nitrogen Removal Rate (NRR) (g N/L·d) 2.0 NRR 1.5 1.0 0.5 0.0 0 50 100 150 200 250 300 350 400 450 500 Time (days) 10

  26. Bioreactor Performance 2.5 Nitrogen Removal Rate (NRR) (g N/L·d) 2.0 NRR 16S Sample 1.5 Metagenome Sample 1.0 0.5 0.0 0 50 100 150 200 250 300 350 400 450 500 Time (days) 10

  27. Next Steps • Verify hypotheses with batch experiments. • Identify strategies to deal with carbon fluctuations at the wastewater treatment plant. Questions? 12

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