By : Ro Romi misaa saa Ad Adel , S l , SIV IV Major or - PowerPoint PPT Presentation

By : Ro Romi misaa saa Ad Adel , S l , SIV IV Major or chemistry mistry Supervis ised by: Pro rof Dr. . M.E.M.Hassouna Profe fessor of f Analytic ical Chemis istry

Heavy metals are re re released fr from in industria ial operations such as ele lectroplatin ing, steel manufacturing, wood preservation, tannin ing, gla lass manufacturin ing and chemic ical pro rocessin ing whic ich are re eventually accumulating and cir irculatin ing throughout the food chain in causing severe problems to humans, anim imals and environment.

Ir Iron is is one of f most sp spre reading heavy metals makin ing up to 5% of f the eart rth cru rust, it it is is one of f the majo jor im impurities that are re commonly pre resent in in many water so sources whic ich cause se se several tro roubles fo for the human health.

Bio iosorption in involves the use se of f mic icrobes to detoxify and to lim limit envir ironmental im impuritie ies and cle lean up polluted sit sites as s well as s usin sing mic icrobial bio iomass as s a bio iosorbent fo for heavy metals re removals

Bacterial and fu fungal bio iomasses are re economic natural adso sorbents fo for metals because they have negativ ive su surface charge and membrane composit ition . The extra racellular polymeric su substances pre resent on the outer su surface of f so some mic icroorganisms contrib ibute to bio iosorption of f metal io ions sin since those poly lymers contain in negativ ively charg rged fu functio ional gro roups, su such as s carb rboxyl, phosphate and su sulphate.

Other sp species that belo long to genus Asp spergillus have been re reported as s hig ighly effi fficient nic ickel s Mucor rouxii and chro romium re reducers su such as fu fungus

The main in in interest of f the pre resent st study is is to in investigate the use se of f A. vers rsicolor re resi sistant fu fungi i powder as s a bio iosorbent fo for the re removal of f iro iron fro from aqueous so solutions as s a fu function of f in init itia ial pH, in init itial metal concentration, temperature and quantity of f the re resi sistant fu fungus.

- Media preparation gi : Isolation ation of met etal l res esis ista tant t fungi It was obtained from Assuit university mycological centre under the AUMC NO. 90, it was grown on czapek yeast extract agar (CYA)

Potato dextrose broth and agar media are prepared using 20g of potato slices boiled with 100 mL of doubly distilled water for 30 min, the filtrate is obtained by strain through several layers of cheesecloth. 2 g of dextrose are added. Then the filtrate up to 100 mL if necessary. For agar plates 1.5 g of agar are added to this mixture, the flask was closed with cotton plug and aluminum foil and autoclaved for 20 minutes at 121ºC.

- Preparation of Fungal Biosorbents Potato Dextrose Agar (PDA) plates have been amended with 100 ppm of Fe (II) ions and the standard spread plate method was performed, the fungi are incubated for 4-7 days at 27 ° C.; the fully grown fungi (resistant metal fungi) have been killed by adding 0.5 N NaOH in a conical flask containing the fungal mat and kept in a water bath for 15 minutes,

the mat was washed with doubly distilled water for about 6-7 times till the pH reaches 7, the mat was then transferred to a sterile Petri dish and placed in hot air oven for 24 hours at 75 ° C, the dried dead fungi are powdered using mortar and pestle, the smaller particle provides larger surface area, and enhancing the biosorption capacity, then stored in sterile container for further study.

Aft fter bio iosorption pro rocess, the re remain ining iro iron io ions in in the so solution are re determined spectrophotometrically, at pH 3-4. Usin sp sing 0.2 % % 1, , 10 phenanthroline so solution, as s a colorim imetric re reagent at 512 nm again inst a bla lank.

Data are expressed as removal percentage of the initial concentration, biosorption q (mg/g) was calculated by the equation: 𝑑 𝑗−𝐷𝑔 𝑟 = 𝑋𝑢 ∗ 𝑊 Where Ci is the initial iron concentration (ppm), Cf is the final concentration (ppm), Wt is the dose of sorbents (g) and V is the volume of solution (mL).

- Figure 1: Effect of Initial pH on Biosorption capacity of iron by A. versicolor resistant powder

25 Effect of Stirring time on Biosorption capacity 20 Biosorption capacity Q (mg/g) 15 10 5 0 0 5 10 15 20 25 30 Stirring time Figure 2: Fig : Eff ffect of of Stir tirring tim time e on on Bio iosorption cap capacity of of iro iron by by A. . ver ersicolor re resi sistant po powder

24 Effect of Metal concentration on Biosorption capacity 22 Biosorption capacity Q (mg/g) 20 18 16 14 12 10 90 100 110 120 130 140 150 Metal ion concentration Fi Figure 3: : Eff ffect of of Metal l co concentration on on Bio iosorption cap capacity of of iro iron by by A. . ver ersicolor re resi sistant po powder

Effect of Temperature on Biosorption capacity 24 22 Biosorption capacity Q(mg/g) 20 18 16 14 12 10 8 6 24 27 30 33 36 39 42 45 Effect of Temperature Fig Figure 4: : Eff ffect of of Tem emperature e on on Bio iosorption cap capacity of of iro iron by by A. . ver ersicolor re resi sistant po powder

Fig Figure 6: : Sca cannin ing el elec ectron mic icrograph of of Asp spergill llus ver ersicolor bio biomass

25 Langmair isotherm plots for the adsorption of iron ions by A. versicolor R ² = 0.961 20 Ce / Qe 15 10 5 0 0 5 10 15 20 25 Ce Fig Figure 7: : Lan angmuir iso isotherm plo plots for for th the ad adsorption of of iro iron io ions by by A. . ver ersicolor

Freundlich isotherm plots for the adsorption of iron ions by A. versicolor R2=0.997 0.40 0.35 0.30 loge Qe 0.25 0.20 0.15 0.10 0.00 0.11 0.22 0.33 0.44 loge Ce Figure 8: Fig : Fre Freundlic ich iso isotherm plo plots fo for th the ads adsorption of of iro iron io ions by by A. . ver ersicolor

Langmuir and Freundlich adsorption isotherm Parameters of iron ions by A.Versicolor S/No. Langmuir isotherm Freundlich isotherm Parameters Parameters Q max (mg/g) 1/n 1 22.25 0.8545 K L K F 2 0.2 1.8 R 2 R 2 0.997 3 0.961 R L 4 0.047 Tab able le 1: : Lan angmuir ir an and Fre Freundli lich ad adsorption iso isotherm Parameters

80 effect of pH on desorption% 70 desorption% 60 50 40 1 2 3 4 5 6 7 8 9 pH Figure 9: Fig : e eff ffect of of pH pH on on de desorption%

effect of stirring time on desorption% 80 70 desorption% 60 50 40 30 20 30 40 50 60 stirring time(minutes) Fig Figure 10 10: e eff ffect of of sti stirrin ing tim time de desorption%

PH 6 Shaking time (minutes) 15 Metal concentration (ppm) 90 Sorbent dose (g) 0.3 Desorption percentage 80% Tab able le 2: : Fac Factors aff affecting th the ex extraction of of iro iron by by A. . ver ersic icolor

- Selected References: 1. Gayatri Y, Shailaja Raj M, Vijayalakshmi B. (2017) Biosorption of lead by Bacillus licheniformis isolated from e-waste landfill, Hyderabad, Telangana, India. International Journal of Bioassays 6. 02: 5240-5244. 2. Siddiquee S, Rovina K, Azad SA, Naher L, Suryani S, et al. (2015). Heavy Metal Contaminants Removal from Wastewater Using the Potential Filamentous Fungi Biomass: A Review. J Microb Biochem Technol 7:384- 393. doi:10.4172/1948-5948.1000243 3. Iram S, Shabbir R, Zafar H and Javaid M. January ( 2015). Biosorption of Copper and Lead by Heavy Metal Resistant Fungal Isolates. International Journal of Scientific and Research Publications, Volume 5, Issue 1. 4. Soleimani N, Fazli MM, Ramazani A and Mehrasbi M. (2016). Application of Live, Dead, and Dried Biomasses of Aspergillus Versicolor for Cadmium Biotreatment J. Hum. Environ. Health Promot. Vol. 1(2):87-98

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