BIOMEDICAL SCIENCE RESEARCH SYMPOSIUM Wednesday 26 October 2016 10:00am to 2:45pm (Followed by prize giving and nibbles) Auckland Hospital, Level 12, Support Building, Rooms 599-12058 and 599-12080
GENERAL INFORMATION The Biomedical Science Research Symposium will be held at the Auckland Hospital, Level 12, Support Building, Grafton, in rooms 599-12058 and 599-12080 on Wednesday 26 th October from 10:00am to 3:00pm. All Honours students are expected to be present throughout the Symposium. There will be two parallel streams of presentations (see map for room locations). Each student will have 15 minutes to present a summary of his or her research and then 5 minutes for questions. Each session will have a chairperson and two official markers who will score each presentation according to the three criteria shown below. The Board of Studies (Biomedical Science) is most grateful to Coherent Scientific and BD Biosciences for providing sponsorship for this event. Presentation Marking Criteria Content ....................................................................................................................................... /30 (Is the context of the work adequately defined) (Quality, quantity and level of information) Structure .................................................................................................................................... /30 (Does it develop as a clear, logical sequence of ideas and conclusions) (Is the audience left with a clear idea of the relevance of the work) Method and delivery ............................................................................................................. /40 (Oral and visual clarity and impact) (Pacing and audience engagement) (Did the presentation go significantly under or over time?) (Response to questions) Total Mark .................................................................................................................... /100 Note: Chairperson will warn presenter when 2 minutes and 1 minute remain. Running a little over time is OK but there will be a penalty if the presentation runs significantly over time. Room numbers for each of the venues and a schematic of their locations are given below. Venue 1 - Room 599-12058 Venue 2 - Room 599-12080 Level 12 can be accessed via the main Auckland City Hospital entrance. Take the escalator to the Level 5 and turn right across the link from the new hospital to the old building. Take the lifts to the level 12. These lifts are located behind the Muffin Break.
VENUE LOCATION Auckland Hospital, Support Building, Level 12 Please use lifts Venue 2 Venue 1 nearer 12080 12058 to 12080
MAP LOCATION AT THE AUCKLAND CITY HOSPITAL
VENUE 1: 599-12058 Chairperson: Dr Scott Graham 10:00am Introduction Time Name Title Supervisor 10:10am Akshata Anchan Migration of melanoma cells across Scott Graham the Blood-Brain Barrier. Features Kate Angel that aid the extravasation of Graeme Finlay melanoma cells into the brain Simon O’Carroll 10:30am Aquila Chua Determining the proliferative and Stefan Bohlander lineage specific properties of CALM- AF10 minimal fusion induced leukemia by the colony forming cell assay 10:50am Brady Cress Toward the inhibition of PI3Kγ Peter Shepherd membrane binding using small Jack Flanagan molecules 11:10am Hannah Darroch Exploring the functional Chris Hall heterogeneity between neutrophils generated during emergency granulopoiesis versus steady state 11:30am Kai-Cheng Deng Use of targeted Proteomics to predict Yongchuan Gu activation of PR-104A in Human Bill Wilson Leukaemias Lunch 12 noon (Room 599-12080) 1:00pm Vittoria Draghi Does rate of rewarming after Laura Bennett hypothermia affect white matter Joanna Davidson integrity after global cerebral ischaemia in the near-term fetal sheep? 1:20pm Blaze Forbes Uptake-2 mediated dopamine Janusz Lipski transport in the nigrostriatal system: Peter Freestone implications for L-DOPA therapy in Parkinson’s disease 1:40pm Amy Gamage The roles of Placental Macrophages Joanna James (Hofbauer cell) in placental vascular development 2:00pm Andrew Jiang Modelling Parkinson’s disease in Renee Hadley sheep 2:20pm Il Hwan Lee Optimizing high throughput Ries Langley screening to discover small molecule inhibitors of a conserved sialic acid binding site in Staphylococcus aureus virulence factors Nibbles and Prize giving – 3:00pm (Room 599-12080)
VENUE 2: 599-12080 Chairperson: Dr Julie Lim 10:00am Introduction Time Name Title Supervisor 10:10am Jessica Liu Cardiac glycogen mishandling in Kimberley Mellor high-fat diet-induced insulin resistance and obesity 10:30am Courtney Lynch Investigating radio sensitivity Francis Hunter determinants in head and neck Bill Wilson cancer using CRISPR-Cas9 10:50am Emma McCafferty Neuroanatomy of GABA A receptors in Henry Waldvogel the normal and Huntington’s disease Richard Faull human cerebellum 11:10am Julian Moxon The effectiveness of cannabinoid Michelle Glass ligands in Melanoma and the Peter Shepherd reliability of commercial melanoma Cell Lines 11:30am Olena Oryshchuk Investigation of the proliferative Stefan Bohlander potential of the BCR/JAK2 fusion gene in primary murine bone marrow cells using an in vitro myeloid clonogenic assay Lunch 12 noon (Room 599-12080) 1:00pm Thulani Palpagama Investigation of GABA signalling in an Andrea in vivo Alzheimer’s disease mouse Kwakowsky model Henry Waldvogel 1:20pm Louise Ramerz The functional characterisation of Marjan Askarian- long non-coding RNA ZFAS1 in Amiri human breast cancer 1:40pm Rebecca Woolley Development of ovine adenovirus as John Taylor a cancer vaccine 2:00pm Vithushilya Characterisation of glutathione Julie Lim Yoganandarajah synthesis pathways in the rat cornea Nibbles and Prize giving – 3:00pm (Room 599-12080)
Venue 599-12058
Presented by: Akshata Anchan Supervisor: Dr Scott Graham and Co-supervisors: Dr Kate Angel, Dr Simon O’Carroll and Dr Graeme Finlay Title: Migration of melanoma cells across the Blood-Brain Barrier. Features that aid the extravasation of melanoma cells into the brain Abstract Background: Melanoma is an aggressive cancer with high propensity to metastasize to the brain. This however, requires disruption of the structural integrity of the blood-brain barrier (BBB), thought to occur through interactions of adhesion molecules between the brain endothelial cells and melanoma cells. Literature surrounding this process using human-cell models is limited, therefore increasing the necessity to investigate the features that may aid cancer metastasis. Methods: This study researched for evidence showing barrier disruption of the BBB after addition of human melanoma cells. Electrical Cell-substrate Impedance Sensing (ECIS) was used to measure this as a decrease in resistance across the tightly adhered brain endothelial cells (hCMVECs). New Zealand melanoma (NZM) cells were also assessed for cell-surface expression of adhesion molecules using flow cytometry while their secretory profiles were assessed using cytometric-bead array. Results: Each NZ Melanoma line induced barrier disruption of hCMVECs within the first few hours of addition and all had high expression of CD44, CD147, CD146 and others but were negative for CD31. Interestingly, preliminary data suggests pronounced difference in a range of secreted factors by the melanoma cells which could influence barrier interactions. Discussion: Understanding the processes by which melanoma cells enter the brain across the BBB has big implications for therapy against aggressive metastatic brain cancers, of which there are no current FDA approved drugs targeting this aspect (migration). As accessibility of drugs to these cells is higher whilst in the blood, discovering targets prior to migration into brain is extremely valuable.
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