BioART & Colr Coli
presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Introduction BioART 1933 - Alexander Fleming “Germ Painting” 2000 - Eduardo Kac “GFP Bunny” BioART beyond the aesthetics presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Object BioART BioSCIENCE Request Making art works Making biosystem by using biomedia as biomedia ex) ex) ・ New tool ・ Color ・ New expression ・ Signal ・ New ethics ・ Motility Stimulus presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Bio media COLOR SIGNAL MOTILITY presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
COLOR presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color Fluorescent protein Pigment presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Fluorescent1 RED RFP CYAN CFP GREEN GFP ORANGE mOrange YELLOW YFP Fluorescent protein’s color p(lac) presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Fluorescent2 YELLOW YFP GREEN GFP CYAN CFP RED RFP ORANGE mOrange Fluorescent protein’s color p(Tet) presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Pigment FPP Lycopene CrtE CrtY ß-Carotene GGPP CrtI CrtB CrtZ Xanthophyll Phytoene Carotenoid biosynthesis pathway presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Pigment Carotenoid Orange presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Future work AKABENI HIWAMOEGI YAMABUKI RINDOU SEIRAN Japanese Traditional Colors presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Color : Future work Color gradation using Signal and Motility’s function presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
SIGNAL presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal For Communication with Environment or Other Cells presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal : Quorum sensing R gene I gene Complex R Protein I Protein Activation AHL Promoter presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal : Object Sender Receiver Function AHL presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal : Characterization LuxI Sender LasI RhlI p(Tet) Inducer CinI LuxR Receiver LasR RhlR p(Tet) Receiver Inducible GFP CinR Promoter Incubated at 37˚C for 24 hours Fluorescence measured at 488/513nm presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal : Result Fluorescence / OD Sender - Receiver Response presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Signal : Future work LuxI LuxR LasI LasR Rhll RhlR CinI CinR Cross Talk presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
MOTILITY presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Propeller Elongation Flagellar Swimming Hook and Junction formation Self assembly of stator Self assembly of rotor Flagellar protein export apparatus presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th Protonic Nanomachine Project, ERATO
Motility : Procedure Previous Target Our Target Flagellar Assembly! Rotational Motor FliH expression in the absence of FliI Protein secretion by fl agellar export FliH apparatus Flagellar Assembly (Minamino, 2008) Substrate presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Motility : Results Inhibition of Flagellar Assembly FliH constitutive Swarming Motility Flagellar Protein Export The motility can be controlled by expressing FliH! presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
MODELING presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Modeling : Equation Cell concentration AHL concentration Growth rate Production rate presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Modeling : Simulation Ex) Triangle model Each cell regulates others as circulation presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Modeling : Signal and Motility Cell Concentration Cell Concentration Distance Distance presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Modeling : Results Without regulation With regulation presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Summary 1. Assembled 10 fluorescence protein parts for germ painting. 2. Assembled 1 pigment parts for germ painting. 3. Devised color gradation systems and developed simulation for it. COLOR 1. Assembled 4 senders and 4 receivers parts. 2. Characterization of 4×4 combination of sender-receiver parts. 3. Modeling the cell motility and development of simulation of triangle model. SIGNAL 1. Developed a new approach to control the motility. 2. Construction and characterization new parts, FliH. 3. Confirmation of expected effect of our parts on swarming motility and protein export. MOTILITY presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Human Practice Artistic usage of biotechnology Bio art raise many bioethical issues will open the potential of it. that are hidden in social system. Bio Art Synthetic Biology Bio art can put the forefront To say that “what is life” , collaboration science in front of the public. of scientist and artist must be needed. presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
Thank you!! To see our works, ・ Poster: Lobby 13, B38 on Sunday ・ Web Gallery: www.microbialart.com ・ E-mail: igemosaka@gmail.com presented by iGEMOSAKA @ iGEM 2009 MIT EG&G Education Center in Boston October 31th
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