The trick in biology is to select, from the thousands of facts that - PowerPoint PPT Presentation

The trick in biology is to select, from the thousands of facts that you know, the three that are relevant to your problem, and to not be disturbed that they contradict each other. Eric Wieschaus (paraphrased) Mark Voorhies 3D Structures

3D Structures Mark Voorhies 4/6/2012 Mark Voorhies 3D Structures

Orienting ourselves s e l e c t a l l c o l o r grey hide e v e r y t h i n g show cartoon Mark Voorhies 3D Structures

Orienting ourselves 2CHT → C → by chain → by chain Mark Voorhies 3D Structures

Pick one trimer in the unit cell s e l e c t a l l hide e v e r y t h i n g s e l e c t trimer1 , chain a+b+c show cartoon , t r i m e r 1 o r i e n t t r i m e r 1 Mark Voorhies 3D Structures

Orient ourselves on the primary structure trimer1 → C → by chain → chainbows Mark Voorhies 3D Structures

Show Conolly solvent accessible surface show s u r f a c e , t r i m e r 1 Mark Voorhies 3D Structures

Run a custom coloring script @ p r o p e r t i e s . pml Mark Voorhies 3D Structures

Find the ligands s e l e c t a l l hide e v e r y t h i n g show cartoon , t r i m e r 1 s e l e c t p r o t e i n , not het s e l e c t ligand , het and not r e s n hoh show s t i c k , l i g a n d Mark Voorhies 3D Structures

Orient on one active site s e l e c t ligA , r e s i 201 o r i e n t l i g A Mark Voorhies 3D Structures

Find the active site residues s e l e c t g1 , p r o t e i n w i t h i n 5.0 of l i g A s e l e c t g2 , b y r e s g1 show s t i c k , g2 Mark Voorhies 3D Structures