See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/311642374 Student Project - Accurate Computer-assisted Cell Culture using Ultrasounds (Presentation) Presentation · December 2016 DOI: 10.13140/RG.2.2.31254.55362 CITATIONS READS 0 40 1 author: Yoann Pageaud German Cancer Research Center 26 PUBLICATIONS 6 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Machine Learning and Cancer Epigenomics View project All content following this page was uploaded by Yoann Pageaud on 14 December 2016. The user has requested enhancement of the downloaded file.
Accurate Computer-assisted Cell Culture using Ultrasounds Yoa oann PAG AGEAUD D – M2BI 13/12/2016
Histoire 1838 : P.F. Verhulst - 1 er Model Croissance Cellulaire 𝑒𝑂 𝑒𝑢 = 𝑠𝑂 1951 : G. Gey - Cellule HeLa 2007 : 1 ère culture cellulaire 3D par ultrasons 2008 : 1 ère culture cellulaire 3D par lévitation magnétique 2009 : 1 ère culture cellulaire 3D par impression
Problématiques Rendements trop aléatoire Nécrose rapide des cellules (Culture 3D) Contaminations Erreurs de Manipulation (Humaine) Solution : Automatiser et Standardiser les Protocoles !
Les Ultrasons : 2 Approches Les Ondes Stationnaires (1975) Le Vortex Acoustique (2016) Transversale Longitudinale :
Matrice Isotrope de Vecteurs ( IVM ) Buckminster Füller (1975) Modèle 3D d’une IVM en PCL Organisation cellulaire 50 µm Perméable aux Ultrasons
Le Robot Ecran (Interface) Pinces Acoustiques Caméras - (x10) Microscopes (x2) Panneaux d’Emetteurs Cartouche de Cellules Ultrasons (x4)
Le Logiciel : Workflow Interface Machine Programme d’Imagerie & Entrée : DOI ou Protocole + Contrôle Qualité Lancement du Protocole Entrée : Lancement Protocole + Piles d’Images Caméras- Sortie : Tableaux Coordonnées Microscopes Programme de Text 3D + Scores Qualité + Caméras Mining Entrée : Protocole de Springer Protocols Programme de Manipulation des Pinces Pinces et des Panneaux Acoustiques Sortie : Paramètres du Entrée : Tableau Coordonnées 3D protocole + Cellules, Matrice et Nœuds de Paramètres ultrasons pression Panneaux Sortie : Panneaux et Pinces puis d’émetteurs Caméras-Microscopes ultrasonores
Equipes de Recherches Equipe 1 – « Acoustique et manipulation de cellule unique » Pince Acoustique Cellule Unique dans IVM Equipe 2 – « Bioinformatiques et culture cellulaire assistée » Développement des Programmes + Conception du Robot Equipe 3 – « Biophysique des ultrasons » Effets des Ultrasons sur les Cellules
Calendrier Prévisionnel 12 Trimestres (36 mois) Equipes Phases 1 2 3 4 5 6 7 8 9 10 11 12 1 1 3 2 3 2 4
Budget Prévisionnel Frais de fonctionnement Administratif Diverse (Salaires, Charges, Sécurité, Impôts) 250 000 € Tests et Prototypage 60 000 € Brevetage 6 000 € Frais d’équipement Matériel d’Acoustique 40 000 € Matériel Informatique 30 000 € Matériel de Biologie (Wet-Lab) 60 000 € Montant Total 446 000 €
MERCI POUR VOTRE ATTENTION !
Diapositives de Réponses aux Questions
Classification des modèles non-linéaires utilisés en culture cellulaire. Al-Rubeai, Animal Cell Culture , 10:265.
Diagramme d’utilisation des polymères naturels (rouge) et synthétiques (bleu), dans le domaine de l’impression 3D Biologique. Atala, Essentials of 3d Biofabrication and Translation , chap. 13.
Ochiai, Takayuki, and Rekimoto , “Three -Dimensional Mid- Air Acoustic Manipulation by Ultrasonic Phased Arrays.”
Baresch, Thomas, and Marchiano , “Observation of a Single - Beam Gradient Force Acoustical Trap for Elastic Particles.”
Bazou et al., “Long -Term Viability and Proliferation of Alginate-Encapsulated 3-D HepG2 Aggregates Formed in an Ultrasound Trap. ”
3D bioplotter system. The plotting material is dispensed into a liquid medium layer by layer. Atala, Essentials of 3d Biofabrication and Translation , p. 158
REFERENCES
Simone Gilgenkrantz, “Regard Sur Soixante Années de Culture de Cellules HeLa ,” Histoire Des Sciences Médicales XLVIII, no. 1 (2014). Ian A. Cree, ed., Cancer Cell Culture , vol. 731, Methods in Molecular Biology (Totowa, NJ: Humana Press, 2011), http://link.springer.com/10.1007/978-1-61779-080-5. Kursad Turksen, ed., Bioreactors in Stem Cell Biology , vol. 1502, Methods in Molecular Biology (New York, NY: Springer New York, 2016), http://link.springer.com/10.1007/978-1-4939-6478-9. Scott H. Randell and M. Leslie Fulcher, eds., Epithelial Cell Culture Protocols , 2nd ed, Methods in Molecular Biology 945 (New York: Humana Press ; Springer, 2012). Anthony Atala, Essentials of 3d Biofabrication and Translation (Boston, MA: Elsevier, 2015). Diego Baresch, Jean-Louis Thomas, and Régis Marchiano, “Observation of a Single-Beam Gradient Force Acoustical Trap for Elastic Particles: Acoustical Tweezers,” Physical Review Letters 116, no. 2 (January 11, 2016), doi:10.1103/PhysRevLett.116.024301. Diego Baresch, “Pince Acoustique: Piégeage et Manipulation D’un Objet Par Pression de Radiation D’une Onde Progressive” (Paris 6, 2014), http://www.theses.fr/2014PA066542. Yoichi Ochiai, Hoshi Takayuki, and Jun Rekimoto, “Three -Dimensional Mid-Air Acoustic Manipulation by Ultrasonic Phased Arrays,” arXiv.org , n.d. D. Bazou et al., “Long -Term Viability and Proliferation of Alginate-Encapsulated 3-D HepG2 Aggregates Formed in an Ultrasound Trap,” Toxicology in Vitro 22, no. 5 (August 2008): 1321 – 31, doi:10.1016/j.tiv.2008.03.014. Mohamed Al-Rubeai, ed., Animal Cell Culture , vol. 9, Cell Engineering (Cham: Springer International Publishing, 2015), p. 297, http://link.springer.com/10.1007/978-3-319-10320-4. Thomas Robert Malthus, Parallel Chapters from the First and Second Editions of an Essay on the Principle of Population (Macmillan, 1909). Turksen, Bioreactors in Stem Cell Biology . Atala, Essentials of 3d Biofabrication and Translation , 6. Al-Rubeai, Animal Cell Culture , 10:265. Atala, Essentials of 3d Biofabrication and Translation , chap. 13.
Glauco R. Souza et al., “ Three-Dimensional Tissue Culture Based on Magnetic Cell Levitation ,” Nature Nanotechnology 5, no. 4 (April 2010): 291 – 96, doi:10.1038/nnano.2010.23. Jennifer R. Molina et al., “Invasive Glioblastoma Cells Acquire Stemness and Increased Akt Activation,” Neoplasia 12, no. 6 (June 2010): 453 – IN5, doi:10.1593/neo.10126. Neenu Singh et al., “Potential Toxicity of Superparamagnetic Iron Oxide Nanoparticles (SPION),” Nano Reviews 1, no. 0 (September 21, 2010), doi:10.3402/nano.v1i0.5358. Ali S. Arbab et al., “Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging,” Radiology 229, no. 3 (December 2003): 838 – 46, doi:10.1148/radiol.2293021215. Farideh Namvar et al., “Cytotoxic Effect of Magnetic Iron Oxide Nanoparticles Synthesized via Seaweed Aqueous Extract,” International Journal of Nanomedicine , May 2014, 2479, doi:10.2147/IJN.S59661. Marcela Gonzales et al., “Cytotoxicity of Iron Oxide Nanoparticles Made from the Thermal Decomposition of Organometallics and Aqueous Phase Transfer with Pluronic F127 ,” Contrast Media & Molecular Imaging 5, no. 5 (September 2010): 286 – 93, doi:10.1002/cmmi.391. Yu Pan, Matthias Bartneck, and Willi Jahnen-Dechent, “Cytotoxicity of Gold Nanoparticles,” in Methods in Enzymology , vol. 509 (Elsevier, 2012), 227. Stefaan J.H. Soenen and Marcel De Cuyper, “How to Assess Cytotoxicity of (Iron Oxide-Based) Nanoparticles. A Technical Note Using Cationic Magnetoliposomes ,” Contrast Media & Molecular Imaging 6, no. 3 (May 2011): 153 – 64, doi:10.1002/cmmi.415. Stefaan J.H. Soenen et al., “Cytotoxic Effects of Iron Oxide Nanoparticles and Implications for Safety in Cell Labelling,” Biomaterials 32, no. 1 (January 2011): 195 – 205, doi:10.1016/j.biomaterials.2010.08.075.
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