Spectral Characteristics of Epicocconone Epicocconone is the fluorophore in: LavaPurple • LavaCell • LavaPep • LavaDigest • FluoroProfile • Lava is a trademark of gelcompany FluoroProfile is a trademark of Sigma Aldrich
Fluorescence on reversible conjugation with proteins and peptides Protein O H 2 N Reaction with proteins O O O causes fluorescent shift OH λ em 530 nm from weak green to O OH intense red / orange protein Protein OH HN fluorescence H O O O Epicocconone OH O OH With Without H Protein O NH protein protein :B O OH O OH λ em 610 nm O O 2 Coghlan et al ., 2005, Org. Lett . 7, 2401-4
Spectral characteristics 16 14 12 Fluorescence 10 532 nm 488 nm 400 nm 8 6 > 200 nm Stokes’ Shift 4 2 0 350 400 450 500 550 600 650 700 750 wavelength (nm) 3 Bell, Karuso (2003). J. Am. Chem. Soc , 125 , 9304-5
Spectral Characteristics of Epicocconone ALP-011005-09-E 4
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