Rare donor program in Japan Yoshihiko Tani, MD, PhD Japanese Red - PowerPoint PPT Presentation

ISBT Working Party on Rare Donors Rare donor program in Japan Yoshihiko Tani, MD, PhD Japanese Red Cross Kinki Block Blood Center Japanese Red Cross Kinki Block Blood Center

Have a national Rare Donor Program Japanese Red Cross Kinki Block Blood Center

Rare Blood in Japan Category I (35) Bombay M k /M /M k En(a – ) MiV/MiV Bombay para- N sat /N /N sat S – /s /s – /U /U – p P k Rhnull Rhmod – D – cD cD – LW(a – b – ) Lu(a – b – ) In(Lu) Ko Kp(a+ b – ) Kp(a – b – ) K14 – k – Kmod McLeod Fy(a – b – ) Jk(a – b – ) I – Ge: – 2, 2, – 3 Lan – IFC – UMC – Dr(a – ) Gy(a – ) Ok(a – ) JMH – Er(a – ) Category II (5) s – Fy(a – b+ ) Di(a+ b – ) Jr(a – ) Do(a+ b – ) Japanese Red Cross Kinki Block Blood Center

Number of Donors in Database Japanese Red Cross Kinki Block Blood Center

Registration of Rare Donors in Japan ( Category I ) as of 2012.3.31 RhD – RhD – Rare Blood RhD + Rare Blood RhD + Bombay 5 0 McLeod 19 0 I – M k M k 1 0 19 0 En(a – ) Ge – 11 0 2 0 Lan – 16 0 MiV / MiV 4 0 Gy(a – ) 6 0 Nsat/Nsat 1 0 Ok(a – ) S – s – U – 19 0 2 1 JMH – 12 0 P k 2 0 － para- Bombay 77 0 Rhnull 5 － p 25 1 Rhmod 7 D – – － Kp(a – b – ) 103 2 0 k – In(Lu) 93 1 8 0 K: – 14 Ko 100 2 2 0 Jk(a – b – ) Fy(a – b – ) 69 1 6 0 Japanese Red Cross Kinki Block Blood Center

Registration of Rare Donors in Japan （ Category Ⅱ ） as of 2012.3.31 Category I RhD – RhD + Rare Blood Fy(a – ) 3,117 495 Di(b – ) 2,671 101 Jr(a – ) 2,130 42 s – 342 106 Japanese Red Cross Kinki Block Blood Center

Number of Rare Blood Donors registered ( Category I ) (1996 - 2011) Japanese Red Cross Kinki Block Blood Center

Number of Rare Blood Donors registered ( Category I ) (1996 - 2011) Japanese Red Cross Kinki Block Blood Center

Number of Rare Blood Donors registered ( Category II ) (2000 - 2011) Japanese Red Cross Kinki Block Blood Center

Number of Frozen Rare Cells Japanese Red Cross Kinki Block Blood Center

Number of Frozen Rare Cells A O B AB as of 2012.3.31 Rare Blood – – – – + + + + numbers Bombay 18 para-Bombay 79 60 16 4 Rhnull 13 Rhmod 9 D – – 89 25 23 20 p 1 3 6 4 P k 3 En(a – ) 8 15 MiV/MiV 7 Ko 50 55 2 51 1 32 McLeod 18 14 32 4 Jk(a – b – ) 53 64 4 26 29 I – 15 10 5 10 Ge – 24 11 13 Lan – 19 20 8 3 Gy(a – ), Hy – 6 1 Ok(a – ) 17 9 6 18 Fy(a – b – ) 14 11 3 1 1 1 In(Lu) 37 4 35 33 7 31

How are Rare Donors found? Japanese Red Cross Kinki Block Blood Center

Monoclonal antibodies for blood grouping (1) Cell line MoAb Cell line MoAb Cell line MoAb anti-P k OSK1 OSK7 OSK15-1 anti-CD44 anti-P anti-U ＋ N OSK1-1 OSK8 OSK15-2 OSK2 OSK9 OSK16 anti-A anti-H anti-Rh29 OSK2-1 OSK9-1 OSK16-1 (anti-Rh17) OSK2-2 OSK10 OSK17 anti-B OSK3 OSK10-1 OSK17-1 anti-P1 OSK3-1 OSK10-2 OSK17-2 OSK3-2 anti-D OSK11 anti-M OSK17-3 OSK18 anti-Le related OSK3-3 OSK12 anti-N anti-Le bH OSK3-4 OSK12-1 OSK18-1 anti-En a FR OSK4 OSK13 OSK19 anti-Fy6 anti-Le a anti-En a FS OSK4-1 OSK13-1 OSK20 anti-En a TS OSK4-2 OSK13-2 OSK20-1 anti-E OSK5 anti-K2 OSK14 anti-I OSK20-2 OSK6 anti-Ge OSK15 anti-P OSK21 anti-Acquired B red ： Human MAb ： rare blood screening Japanese Red Cross Kinki Block Blood Center

Monoclonal antibodies for blood grouping (2) Cell line MoAb Cell line MoAb Cell line MoAb anti-Kp b OSK36 OSK22 OSK29-2 anti-Di a OSK37 OSK22-1 anti-Fy3 OSK29-3 anti-Xg a OSK22-2 OSK29-4 OSK38 anti- GPA&B OSK23 anti-T OSK29-5 OSK39 anti-IFC anti- Miltenberger OSK40 OSK24 OSK29-6 anti-Tn anti-Fy b OSK41 OSK24-1 OSK29-7 OSK25 OSK29-8 anti- GPA OSK42 anti-e anti-K14 anti-Jr a OSK30 OSK43 anti-Lan OSK25-1 anti-Di b OSK26 anti-c OSK31 OSK44 anti-Hro OSK27 anti-Tk OSK32 anti-K5 OSK46 anti-Kg (Ku) anti-Kp c anti-Fy a OSK28 anti-i OSK33 OSK47 OSK29 OSK34 anti-K1 OSK48 anti-C anti-GPA&B anti-Jk b OSK35 OSK49 anti-S OSK29-1 OSK-MoAb: 49 kinds, 93 cell lines red ： Human MAb ： rare blood screening Japanese Red Cross Kinki Block Blood Center

Blood Grouping Reagent : Cross-linked Monoclonal antibody mix Human MoAb Mouse anti-human IgG MoAb Red Blood cells agglutination Cross-linked MoAb Japanese Red Cross Kinki Block Blood Center

Total volume of monoclonal antibodies supplied to other blood centers （ 1987-2009 ） No. of No. of Blood Total volume Year MoAbs centers supplied (mL) 1987 6 19 21,890 1988 8 23 18,290 1987~ ： anti-En a , anti-Hro(Rh17) 1989 14 19 15,540 anti-K2, anti-Ge 1990 15 21 16,840 anti-U+N, anti-CD44 1991 20 23 19,845 1992 24 23 20,460 1993 24 22 18,045 1991~ ： anti- I 1994 27 30 30,348 1994~ ： anti-Jr a , anti-Di b 1995 28 45 50,301 1996 30 41 59,018 2005~ ： anti-Lan 1997 26 31 49,187 1998 31 36 32,217 1999 27 25 28,655 2007~: Consolidating of laboratory 2000 36 28 39,731 2001 31 32 56,962 2002 34 34 75,770 ・ Screening of rare blood 2003 32 34 77,005 ・ Screening of antigen negative blood 2004 30 37 87,590 2005 37 34 111,910 ・ Blood typing 2006 34 28 97,630 2007 33 19 125,050 2008 35 12 255,365 2009 28 10 330,512 2010 32 10 397,204 Japanese Red Cross Kinki Block Blood Center 2011 39 10 455,610

Automated machine PK7300 Japanese Red Cross OSAKA Blood Center

Images of hemagglutination on PK7300 D ABO antibody rare blood detamination D − Ab− A 1 Ab − D ＋ A 2 Ab − D ＋ A 3 Ab − D ＋ O 4 Ab − D ＋ O 5 Ab − D ＋ O 6 Ab − D ＋ 7 B Ab − D ＋ 8 B Ab − 9 AB D ＋ Ab − 10 AB D ＋ Japanese Red Cross Kinki Block Blood Center

Number of Rare Donor units used per year Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Provided ( Category I ) (1997 – 2011) Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Provided ( Category I ) (2000 – 2011) units 60 2011 50 40 30 20 10 0 D – – Jk(a – b – ) p Ko Bombay (para) Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Provided ( Category II ) (2000 - 2011) Units 1.000 2011 800 600 400 200 0 Jr(a – ) Fy(a – ) Di(b – ) Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Internationally shipped (1977 – 1998) year country rare blood units year country rare blood units USA Di(b – ) MkMk 6 Germany(W) 1977 6 Germany(W) 1988 Ko 8 Di(b – ) 1981 Germany(W) 16 Thailand Bombay 4 D – – USA 19 Jr(a – ) 1982 USA 4 Di(b – ) Germany(W) 9 Germany(W) 1989 Ko 4 Di(b – ), Ccee, Fy(b-) Germany(W) 6 Di(b – ) Thailand 14 Di(b – ), Jk(b-) Germany(W) 1983 13 Thailand 1991 Bombay 7 D – – France 12 Di(b – ) Australia 8 Thailand 5 Bombay(Para-Bombay) 1995 1984 Thailand Ko 9 Di(b – ), Jk(b-) Germany(W) 16 Di(b – ) 1996 Sweden 8 Thailand p 4 1985 Di(b – ), Jk(b-) Germany(W) 10 Jk(a – b – ) 11 Taiwan 1997 D – – Di(b – ), Jk(b-) USA 6 20 Di(b – ) 1986 Germany(W) 9 Switzerland Ko 8 Ko 6 Jr(a – ) Norway 8 1998 D – – Argentina 4 Germany(W) 1987 Ko 6 Jr(a – ) UK 4 Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Internationally shipped (1999 – 2012.3) year country rare blood units year country rare blood units En(a – ) Sweden 12 Thailand Ko 8 2006 Jr(a – ) USA Fy(a – ), C – 1999 4 China 16 Netherlands Ko 10 D – – 2007 New Zealand 6 Indonesia 10 Para-Bombay Di(b – ), D – 2000 Netherlands 4 D – – UK 6 2008 Thailand 12 Fy(a-),C-,e-,Jk(b-) Di(b – ) China 32 2001 Di(b – ), D – Netherlands 4 D – – Canada 2 2009 Thailand Fy(a-),C-,e-,Jk(b-) 12 Jk(a – b – ) 2002 Philippine 2 p, Fy(b – ) Malaysia 2 D – – 2003 Spain 8 D – – UK 4 2010 Jk(a — b – ) Australia Philippine p 4 12 Jr(a – ) USA 40 Canada Ko 11 D – – Korea 2004 8 D – – UK 2011 8 D – – Brazil 6 D – – New Zealand 4 Ko, D – Brazil 4 Lan – Australia 2012 4 Thailand p 10 D – – 2005 8 Czech Republic Jk(a – b – ) Philippine 15 Japanese Red Cross Kinki Block Blood Center

Units of Rare Blood Internationally shipped – 1 (1977 – 2012.3) country year rare blood units country year rare blood units 1999 Ko 10 Di(b – ) 1977 6 Netherlands Di(b – ), D – 2009 4 Di(b – ) 1981 16 Di(b – ) 1982 9 Ko, c – E – ,Fy(b-) Switzerland 1998 6 Di(b – ), Fy(b-),Ccee 1983 6 Jr(a – ) 1998 4 Di(b – ), Jk(b-) 13 D – – 2000 6 Di(b – ), Jk(b-) UK 1984 16 D – – Germany 2010 4 Di(b – ), Jk(b-) 1985 10 D – – (West) 2011 8 Di(b – ), Jk(b-) 1986 20 D – – Spain Di(b – ) 2003 8 9 Ko 6 D – – 2005 8 Czech Republic 1987 Ko 6 D – – 1982 19 1988 Ko 8 M k M k 1988 6 1989 Ko 4 Jr(a – ) USA 4 Jr(a – ) D – – 2004 40 France 1983 12 Jr(a – ) 2005 16 Jr(a – ) Norway 1998 8 D – – , Fy(b – ), Jk(a – ) 2001 2 Di(b – ), C – , S – , P 2 Canada 1996 8 Sweden 2011 11 Ko En(a – ) 1999 12 Japanese Red Cross Kinki Block Blood Center