our goal to encourage growth and expansion of the igem
play

Our goal: to encourage growth and expansion of the iGEM competition. - PDF document

May 06, 2023 •289 likes •378 views

06/11/2010 Our goal: to encourage growth and expansion of the iGEM competition. What are the limits of the most common One cell = one compartment. iGEM chassis ( E. coli )? 1 06/11/2010 Plasmid capacity. Movement speed and range. 2

  1. 06/11/2010 Our goal: to encourage growth and expansion of the iGEM competition. What are the limits of the most common One cell = one compartment. iGEM chassis ( E. coli )? 1

  2. 06/11/2010 Plasmid capacity. Movement speed and range. 2

  3. 06/11/2010 Bacteria ( E. coli ) C. elegans Agar plates Easy maintenance Short life cycle Antibiotic selection Glycerol stocking Familiar? SID-2 (dsRNA transporter) A property of many promoters 3

  4. 06/11/2010 promoter brick coding brick terminator • Superior locomotion • Extensive sensory capabilities • Take advantage of E. coli parts 4

  5. 06/11/2010 Inter-tissue communication (e.g. nervous system to muscular system) Ion channels: • Channelrhodopsin-2 (ChR2) • Halorhodopsin (NpHR) Tissue-Specific Constitutive pMec-7 mechanosensory neurons pGpd-2 > pRab-7 > pSip-1 pOdr-10 AWA olfactory neuron pStr-220 AWB olfactory neuron pStr-1 AWB chemosensory neuron Inducible pOdr-1 AWC chemosensory neuron pOsm-10 ASH osmosensory neuron pHsp-3 (enhanced by chemical stress) pFlp-1 AVA sensory neuron pSra-10 AVB sensory neuron 5

  6. 06/11/2010 eGFP Green fluorescence marker eCFP Cyan fluorescence marker eYFP Yellow fluorescence marker mCherry Red fluorescence marker STOP ChR2 Channelrhodopsin-2 unc-54 3' UTR contains poly-A signal NpHR Halorhodopsin BBa_K309000 pMec-7 Promoter BBa_K309012 unc- T erminator BBa_K309001 pSra-10 Promoter BBa_K309013 pOsm-10 Promoter BBa_K309002 pGpd-2 Promoter BBa_K309014 pOdr-1 Promoter BBa_K309003 pStr-1 Promoter BBa_K309015 NpHR::eCFP T agged effector BBa_K309004 ChR2::eYFP T agged effector BBa_K309016 eGFP Reporter BBa_K309005 mCherry Reporter BBa_K309017 pStr-220 Promoter BBa_K309006 pSip-1 Promoter BBa_K309018 eYFP Reporter BBa_K309007 ChR2 Effector BBa_K309019 NpHR Effector BBa_K309009 pOdr-10 Promoter BBa_K309020 eCFP Reporter BBa_K309010 pFlp-1 Promoter BBa_K309021 pStr-220::eCFP Full construct BBa_K309011 pHsp-3 Promoter BBa_K309022 pOdr-10::eCFP Full construct 6

  7. 06/11/2010 ALML PLML AVM BBa_K309023 pGpd-2::eCFP Full construct BBa_K309024 pSip-1::eCFP Full construct PVM ALMR PLMR BBa_K309025 pFlp-1::eCFP Full construct BBa_K309026 ChR2::eYFP T agged effector + terminator BBa_K309027 pHsp-3::eCFP Full construct Part:BBa_K309032 BBa_K309028 pOsm-10::eCFP Full construct pMec-7::eCFP::unc-54 3' UTR BBa_K309029 mCherry Reporter + terminator BBa_K309030 eYFP Reporter + terminator BBa_K309031 pOdr-1::eCFP Full construct BBa_K309032 pMec-7::eCFP Full construct PLMR Kozak sequence around start codon GCC ACC AUG G Optimizes translation 7

  8. 06/11/2010 • WormBase huge gene databank • WormBook thorough research review/textbook • WormAtlas where to start with worms • WormMethods protocols • GFPWorm where promoters function • Promoterome known minimal promoters • UTRome and UTRdb 3' UTRs • Feasibility? • Dr. Ian Chin-Sang, and all of our other advisors • • VWR, for discounts on reagents • Fundamental parts • Octane Biotech, for their generous • New standard contribution • WormGuide • -going • Go forth! support • And you, for watching. 8

Recommend

C F L T R I C L U B G O A L The goal of the triathlon club is to encourage good health and

C F L T R I C L U B G O A L The goal of the triathlon club is to encourage good health and

2 0 1 6 - 1 0 T H A N N U A L K I C K O F F M E E T I N G C F L T R I C L U B G O A L The goal of the triathlon club is to encourage good health and fitness, success, and create a sense of belonging. Members encourage one another,

116 views • 9 slides
Broadband Expansion Grant Program FY 2018 Round 2 Broadband Expansion Grant Webinar Dennis

Broadband Expansion Grant Program FY 2018 Round 2 Broadband Expansion Grant Webinar Dennis

Broadband Expansion Grant Program FY 2018 Round 2 Broadband Expansion Grant Webinar Dennis Klaila & Jaron McCallum Public Service Commission of Wisconsin November 2017 Broadband Expansion Grant Program Purpose: To encourage the

419 views • 25 slides
Illinois iGEM 2009 Bacterial Decoder Our Goal To create a universal, modular , and dynamic

Illinois iGEM 2009 Bacterial Decoder Our Goal To create a universal, modular , and dynamic

Illinois iGEM 2009 Bacterial Decoder Our Goal To create a universal, modular , and dynamic bacterial decoder to be used as a tool in a variety of cellular logic applications, while also contributing novel BioBricks to the Registry of Standard

512 views • 24 slides
Choosing Wisely ABIM Foundation initiative Goal to encourage physicians, patients and

Choosing Wisely ABIM Foundation initiative Goal to encourage physicians, patients and

Choosing Wisely ABIM Foundation initiative Goal to encourage physicians, patients and other health care stakeholders to think and talk about medical tests and procedures that may be unnecessary, and possibly harmful 47 specialty

402 views • 12 slides
UK money growth in the long expansion: what can it tell us about the role of money ? Michael Mcleay

UK money growth in the long expansion: what can it tell us about the role of money ? Michael Mcleay

UK money growth in the long expansion: what can it tell us about the role of money ? Michael Mcleay Ryland Thomas The Causes and Consequences of the Long UK Expansion Conference 20 th September 2013 Background Since the financial crisis began a

629 views • 34 slides
E. chrono Vanderbilt Microfluidics iGEM 2014 New Track, New Team Vanderbilt iGEM was formed

E. chrono Vanderbilt Microfluidics iGEM 2014 New Track, New Team Vanderbilt iGEM was formed

E. chrono Vanderbilt Microfluidics iGEM 2014 New Track, New Team Vanderbilt iGEM was formed this past February by a group of freshmen interested in research Collaboration between Vanderbilt iGEM and the Vanderbilt Institute for

512 views • 18 slides
BUCT-China iGEM 2014 TOXINMETER iGEM 2014 Diseases My name is

BUCT-China iGEM 2014 TOXINMETER iGEM 2014 Diseases My name is

BUCT-China iGEM 2014 TOXINMETER iGEM 2014 Diseases My name is Hello e.g. Detec3on Minamata disease Normal methods to detect the water quality Gas

541 views • 31 slides
Heuristic Planning with Single Action Goal Expansion Remo Christen Department of Mathematics and

Heuristic Planning with Single Action Goal Expansion Remo Christen Department of Mathematics and

Heuristic Planning with Single Action Goal Expansion Remo Christen Department of Mathematics and Computer Science University of Basel 5.7.2019 Introduction SAGE Results Conclusion Classical Planning Initial state Goal a 0 a 1 a 2 a n .

665 views • 37 slides
iGEM 2015 2015 Cooper Union Summer STEM Program

iGEM 2015 2015 Cooper Union Summer STEM Program

iGEM 2015 2015 Cooper Union Summer STEM Program http://www.cmu.edu/bio/research/undergrad_research/summer/igem/images/igem.png Biosensors An analytical device that detects a substance, and is made from biological parts that are based on or

888 views • 52 slides
Northwestern U University iGEM iGEM 20 2013: 3: A n novel a approach t to o oral h

Northwestern U University iGEM iGEM 20 2013: 3: A n novel a approach t to o oral h

Northwestern U University iGEM iGEM 20 2013: 3: A n novel a approach t to o oral h health The Problem: Tooth Decay Tooth Decay: Quick Facts 36% of people worldwide have dental caries in their

743 views • 55 slides
iGEM 2014 Giant Jamboree 10/31/14 Our Team 1 2 Worcester Polytechnic Institute Overall

iGEM 2014 Giant Jamboree 10/31/14 Our Team 1 2 Worcester Polytechnic Institute Overall

Ag-goat-ination: Combating Antibiotic Resistance through Diagnostic Agglutination Assays iGEM 2014 Giant Jamboree 10/31/14 Our Team 1 2 Worcester Polytechnic Institute Overall Project Goal Design a New Method for Disease Diagnostics

628 views • 30 slides
UT iGEM 2012: Caffeinated coli h7p://2012.igem.org/Team:AusAn_Texas

UT iGEM 2012: Caffeinated coli h7p://2012.igem.org/Team:AusAn_Texas

UT iGEM 2012: Caffeinated coli h7p://2012.igem.org/Team:AusAn_Texas Caffeine P. putida CBB5 Addiction Refactored operon Measurement Inducible promoter Caffeine Caffeine is 1,3,7-trimethyl xanthine

590 views • 36 slides
Savannah Harbor Expansion Project Critical Infrastructure Expansion for the United States May 3,

Savannah Harbor Expansion Project Critical Infrastructure Expansion for the United States May 3,

Savannah Harbor Expansion Project Critical Infrastructure Expansion for the United States May 3, 2017 1 SAVAN SA ANNAH AH: #4 AND FASTEST GROWING TOP TOP 1 10 POR ORTS TS: 1 10-YEAR GRO GROWTH RAT RATE CY2006 2006-20 2016 16

400 views • 16 slides
Goal of a headline is to encourage someone to read your story, announcement, news brief, etc.

Goal of a headline is to encourage someone to read your story, announcement, news brief, etc.

Headlines Writing Tips Goal of a headline is to encourage someone to read your story, announcement, news brief, etc. Headlines Writing Tips Include a number Five Ways to Have a Faith-Filled Lent Headlines Writing Tips Ask a question Do you

354 views • 13 slides
An iGEM-Compliant Shu3le Vector for Clostridial Chassis

An iGEM-Compliant Shu3le Vector for Clostridial Chassis

An iGEM-Compliant Shu3le Vector for Clostridial Chassis Organisms The Oklahoma iGEM Team Jeremiah Miller, Erin Weese, Zachariah Herron, Samuel Jones, JusDn

625 views • 24 slides
Factors Which affect Nucleation, bubble growth and Expansion During Extrusion impact on

Factors Which affect Nucleation, bubble growth and Expansion During Extrusion impact on

Factors Which affect Nucleation, bubble growth and Expansion During Extrusion impact on mechanical properties and crispness Jozef L.Kokini Department of Food Science Whistler Carbohydrate Center Purdue University Outline of

586 views • 44 slides
A GENETIC LIMITER CIRCUIT IN S. CEREVISI BROWN 2.0 BROWN 2.0 IGEM IGEM 2008 2008 J.

A GENETIC LIMITER CIRCUIT IN S. CEREVISI BROWN 2.0 BROWN 2.0 IGEM IGEM 2008 2008 J.

A GENETIC LIMITER CIRCUIT IN S. CEREVISI BROWN 2.0 BROWN 2.0 IGEM IGEM 2008 2008 J. SZYMANSKI SZYMANSKI A. GLIEB A. GLIEBERMAN RMAN An Ideal Limiter Protects against signal overload Limits input to a given threshold Leaves

398 views • 28 slides
1 DSOs growth model: the cornerstone figure for the expansion of natural gas Gas Promotion

1 DSOs growth model: the cornerstone figure for the expansion of natural gas Gas Promotion

1 DSOs growth model: the cornerstone figure for the expansion of natural gas Gas Promotion Spain Gas Distribution Business Madrid, march 5 2 Appendix 1. Spanish market conditions 2. Unbundling 3. Promotion and MKT development

505 views • 16 slides
2016 Presentation to Analysts HIGHLIGHTS HIGHLIGHTS MERGER OF CONTINUING LARGE EXPANSION

2016 Presentation to Analysts HIGHLIGHTS HIGHLIGHTS MERGER OF CONTINUING LARGE EXPANSION

HALF-YEAR RESULTS 2016 Presentation to Analysts HIGHLIGHTS HIGHLIGHTS MERGER OF CONTINUING LARGE EXPANSION GABON TELECOM STRONG GROWTH OF THE 4G+ NETWORK AND MOOV GABON INTERNATIONALLY CONTINUING GROWTH IN GROWTH IN CAPITAL

570 views • 28 slides
METERED BILLING PROPROSAL GOAL: Establish fair water charges that encourage water conservation

METERED BILLING PROPROSAL GOAL: Establish fair water charges that encourage water conservation

August 29, 2014 METERED BILLING PROPROSAL GOAL: Establish fair water charges that encourage water conservation & remain close to historic fees. DISCUSSION: Using the HISTORIC GENERAL FUND AVERAGE we can determine other FUND charges by HISTORIC

325 views • 14 slides
Globalization of Service ShinMing Guo NKFUST Domestic Growth & Expansion

Globalization of Service ShinMing Guo NKFUST Domestic Growth & Expansion

Globalization of Service ShinMing Guo NKFUST Domestic Growth & Expansion Franchising International Strategies Planning International Operations Is Globalization Good? Yum! Brands, Inc. Owns KFC, Pizza

165 views • 12 slides
Biofilm Killer BIOSCIENCES LYON iGEM TEAM 2012 Context iGEM Synthetic Biology for Innovative

Biofilm Killer BIOSCIENCES LYON iGEM TEAM 2012 Context iGEM Synthetic Biology for Innovative

Biofilm Killer BIOSCIENCES LYON iGEM TEAM 2012 Context iGEM Synthetic Biology for Innovative Solutions competition Context Industrial biofilm issues Oil industry : Osli, Schlumberger Cosmetics: Gatefoss Enzyme-based cleaning solutions:

518 views • 51 slides
REACH Appalachia Rural transit Expansion for Advanced Community Health Project Overarching Goal

REACH Appalachia Rural transit Expansion for Advanced Community Health Project Overarching Goal

REACH Appalachia Rural transit Expansion for Advanced Community Health Project Overarching Goal Facilitate the development of a local, inclusive coordinated transportation system in rural eastern Kentucky and southern Ohio, in which

716 views • 10 slides
e.g. Taq polymerase PCR TEAM HEIDELBERG iGEM 2014 2.0 PCR 2. maintaining DNA

e.g. Taq polymerase PCR TEAM HEIDELBERG iGEM 2014 2.0 PCR 2. maintaining DNA

Heat stable proteins are valuable for Industrial processes Research applications e.g. Taq polymerase PCR TEAM HEIDELBERG iGEM 2014 2.0 PCR 2. maintaining DNA methylation patterns PCR TEAM HEIDELBERG iGEM 2014 2.0

819 views • 51 slides

More recommend