Modeling Wetlab Medical Engineering Human practices Male patient - PowerPoint PPT Presentation

Modeling Wetlab Medical Engineering Human practices

Male patient 11 years old

Diagnosis ?

GGBP Vyas N, Vyas M, Quiocho F (1988) Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein. Science. 242, (4883) pp. 1290 - 1295

Trg two component system Baumgartner, et al. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." J Bactriol. (1994)

EnvZ two component system Feng X, Oropeza R, Walthers D, Kenney L (2003). OmpR Phosphorylation and Its Role in Signaling and Pathogenesis. ASM News, 69, (8). pp. 390-395

TrgZ chimera Baumgartner, et al. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." J Bactriol. (1994)

Modeling

Negative feedback system

Bacterial Growth

Glucose-insulin Dynamics

Engineering • Great value. • Functionality. • Easy of use. • Great design.

SAFETY OF THE USER

Modular arrangment

Contention Module Polymer such as birth control subdermal implant or biocompatible PET/PE

Communication Module Material: stainless steel- X15Cr13 and polymer of EPP-PEEK Ketron LSG

Iris mechanism simulation

External Module

Finite Element Analysis

Five bars with eccentric gear mechanism

Mechanism simulation

External module simulation

Results Three E. coli strains were used: EnvZ knock-in, Rosettagami* and SHuffle* ✓ Strains’ growth curves characterization. ✓ Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓ Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓ Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Results ✓ Strains’ growth curves characterization. ✓ Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓ Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓ Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Reporter BBa_M30011 ✓ Strains’ growth curves characterization. ✓ Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓ Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓ Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Biobrick Design gBlock synthesis Restriction Gibson analysis assembly Transformation ✓ Strains’ growth curves characterization. ✓ Measurement of the response to different glucose levels of EnvZ knock-in strain with Western a reporter gene. Blot ✓ Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓ Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

✓ Strains’ growth curves characterization. ✓ Measurement of the response to different glucose levels of EnvZ knock-in strain with a reporter gene. ✓ Assembly of a codon optimized TrgZ chimera for E. coli as well as a proinsulin lispro part with a TAT secretion signal. ✓ Constitutive expression of proinsulin lispro in SHuffle and Rosettagami strains.

Collaboration with UNAH

Collaboration with UNAH

Collaboration with UNAH

UNAH-UNAM

Human Practices

Talks about the model at RIIEME

Modeling Wetlab Medical Engineering Human practices

Credits Team Members Alejandro Guzmán - Storyteller Frida Reyes - Physician Lizbeth Bolaños - Researcher Alfredo López - Mathematical biologist Diego Galavíz - Engineer Xrysw Hernández - Accomplishments mentioning Alejandro Rodríguez Carlos Gil César Carreón Acknowledgments Advisors Gloria Soberón Pablo Padilla Roberto Arreguín Marco Herrera Javier Mancilla Eduardo Rodríguez Joanna Hernández José Alonso Roberto Arreguín

u [1] "Getting Syrian children back to school in Lebanon (11174110673)" by DFID - UK Department for International Development - Getting Syrian children back to school in LebanonUploaded by russavia. Licensed under CC BY 2.0 via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Getting_Syrian_children_back_to_school_in_Leban on_(11174110673).jpg#/media/File:Getting_Syrian_children_back_to_school_in_Lebanon_(11 174110673).jpg •[2] "Hospital room ubt" by Tomasz Sienicki [user: tsca, mail: tomasz.sienicki at gmail.com] - Own work. Licensed under Public Domain via Wikime Commons - https://commons.wikimedia.org/wiki/File:Hospital_room_ubt.jpeg#/media/File:Hospital_room_ubt.jpeg [3]"Insulin". Licensed under CC BY-SA 3.0 via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Insulin.jpg#/media/File:Insulin.jpg [4]"Alpha-D-Glucopyranose" by NEUROtiker - Own work. Licensed under Public Domain via Wikimedia Commons - https://commons.wikimedia.org/wiki/File:Alpha-D-Glucopyranose.svg#/media/File:Alpha-D-Glucopyranose.svg