Introduction of an open-source, multi-material bioprinting platform - PowerPoint PPT Presentation

Introduction of an open-source, multi-material bioprinting platform 3D bioprinting conference - 26 January 2016 - Maastricht

Overview www.teamhelloworld.com 1.CANTER at a glance 2.AM in medicine 3.Open issue 4.Why open-source? 5.Bioprinting platform 6.Research results 7.Conclusion Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introduction: CANTER CANTER Center for Applied Tissue Engineering and Regenerative Medicine Development of new methods and applications for tissue engineering. Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introdution: CANTER User Engineer (Life Sciences) Understands the requirements Knows how to build devices Makes existing things better Development of own, efficient workflow Low access barrier Problem solver / no over-engineering Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Introdution: CANTER Mechatronics Bioengineering Clinical Sciences Mechanical Engineering Biology Biophysics Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine Exoprosthesis www.3dprintingindustry.com Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine Exoprosthesis Endoprosthesis www.ke-next.de Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine Exoprosthesis Endoprosthesis Bioscaffolding www.nationalgeographic.com Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine Exoprosthesis Endoprosthesis Bioscaffolding www.storiesbywilliams.com Biofabrication Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Additive manufacturing in medicine Exoprosthesis Endoprosthesis Bioscaffolding www.storiesbywilliams.com Biofabrication Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Open Issues - Lack of established, easy-to-use BioInks - Huge amount of theories / ideas, that have to be tested - Field of basic research - Expensive alternatives - Fast, evolving field of additive manufacturing www.inlingua.com Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Why open-source? - Integration of open-source hardware & software in own projects: - Marlin Software - Arduino Hardware - Great, constantly evolving community - Low access barriers - Simple, highly efficient interfaces www.wikipedia.de Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Bioprinting platform - Low-cost platform - User-friendly - Enabling a huge variety of experiments, especially in the field of basic research - Standard interfaces - Highly customizable Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Current development - Automatic bedleveling - Incubator unit: - Gasparameters: - Carbon dioxide - Nitrogen - Temperature - Humidity - LED integration - Touchscreen interface - Live data - IR cam Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Modularity - Different usable printheads: - Mechanically driven - Pneumatically driven - Different extrusionmethods: - Mono-extrusion - Dual-extrusion - Different cross-linking methods: - UV-light, green-light - Calcium chloride - Different printbeds: - Petridish - Multiwell-plate - Non-submersive printing - Submersive printing Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Multi material - Huge variety of hydrogels, regardless of their characteristics and needs - Different scaffold materials - Extrusion-methods: - Standard, single extrusion - Mixing - Strand next to strand - Strand on strand - Material enclosing Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Results Live / Dead Assay hMSCs Before printing After printing Green: living cells Red: dead cells 5% (w / v) alginate hydrogel Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Results Live / Dead Assay hMSCs Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Results Live / Dead Assay Print accuracy hMSCs Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Results Live / Dead Assay Print accuracy hMSCs Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Conclusion www.teamhelloworld.com - Powerful, easy-to-use device - Enabling a huge variety of experiments - Ensuring critical incubation parameters Outlook: - Improve usability (automated slicing software) - Release of CAD and software files Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu

Special Thanks to … • Daniel Baier • Julian Blaser • Hauke Claussen-Schaumann • Liam Daly • Jakob Denzel • Alfred Fuchsberger • Max Hörter • Benedikt Kaufmann • Manuel Oestreich • Tobias Petzinger • Rainer Stabhuber • Johannes Stepper • Stefanie Sudhop Thank you very much for your attention! • Joseph Thaler Sascha Schwarz CANTER , Center for Applied Tissue Engineering and Regenerative Medicine schwar15@hm.edu