In vivo stepwise immunomodulation using chitosan nanoparticles as a - PowerPoint PPT Presentation

The 15 th Korea-U.S. Forum on Nanotechnology In vivo stepwise immunomodulation using chitosan nanoparticles as a platform nanotechnology for cancer immunotherapy Hee Dong Han School of Medicine, Konkuk University

DC-based cancer immunotherapy Injection of DCs Mature DC Immature DC PLGA-NPs Intracellular delivery of antigen and adjuvant CD 8+ T cell activation co-encapsulated PLGA-NPs Tumor microenvironment Inhibition of tumor cells growth Engineered Nanoparticle Tumor cell

Overall concept Lymph node Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Physical properties of CH-NPs Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Intracellular delivery of CH-NPs to DCs Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Maturation and activation of DCs induced by CH-NPs Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

In vivo stepwise CH-NP-based active immune response Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Therapeutic efficacy of CH-NP treatment in EG.7 tumor model Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Summary - We demonstrated that a novel in vivo active cancer immunotherapy based on CH-NP loaded with an adjuvant and antigen to increase in vivo maturation of DCs lead to potent antigen-specific CD8+ T cell immunity. - Our CH-NP system could be expanded and developed to include additional therapeutic and experimental approaches.