21/07/54 Oral Presentation CRDC 5 USE OF CHITOSAN TO PROLONG POSTHARVEST STORAGE OF LIME UNDER DIFFERENT CONDITIONS Department of Botany, Faculty of Science Chulalongkorn University กราฟแสดงราคามะนาวเฉลี�ยต่อลูก ที�เกษตรกรจําหน่ายได้ในรอบปี 2552 10 9 8 7 6 ราคา / ลูก 5 4 3 2 1 0 ที�มา : สํานักงานภายในจังหวัดสมุทรสาครปี 2552 1
21/07/54 Oral Presentation CRDC 5 Postharvest loss of lime ที�มา : http://hilight.kapook.com/admin_hilight/spaw2/ imghilight4/news/1_92.jpg 2
21/07/54 Oral Presentation CRDC 5 Low molecular weight chitosan (0.2%) more effective in controlling the growth of fungus on citrus fruits more effective in controlling the growth of fungus on citrus fruits caused by Penicillium digitatum and Penicillium italicum caused by Penicillium digitatum and Penicillium italicum � improved firmness � improved firmness � titratable acidity � titratable acidity � ascorbic acidity � ascorbic acidity � water content for Murcott tangor stored at 15ºC for 56 � water content for Murcott tangor stored at 15ºC for 56 days. (Po-Jung Chien et al., 2005) days. (Po-Jung Chien et al., 2005) 3
21/07/54 Oral Presentation CRDC 5 Application of 2 g chitosan/100 g solution Chitosan on navel orange (2%) Objective To determine the induction of disease resistance in lime fruit by chitosan and effect on postharvest quality of lime. 4
21/07/54 Oral Presentation CRDC 5 Part 1 Effect of chitosan treatments on postharvest quality of lime after storage at 25°C for 4 weeks Part 2 Effect of chitosan treatments on postharvest quality of lime after storage at 10°C for 6 weeks % Weight loss Peel color change by Minolta Chroma Meter (CR - 10) P l l h b Mi lt Ch M t (CR 10) Disease appearance scale ranging from 1 to 5 1 = less than 5% disease appearance or no damage 2 = 5-30% disease appearance 3 = 31-60% disease appearance 4 = 61-90% disease appearance 5 = more than 90% disease appearance (Smilanick et al.,2006) 5
21/07/54 Oral Presentation CRDC 5 Effect of chitosan treatments on postharvest quality of lime after storage at 25°C for 4 weeks Part 1 Part 1 25ºC 5 min Distilled water (control) ( t l) 5 ppm 5 sub-replicates in 4 replicates 0 ppm 15 ppm 6
21/07/54 Oral Presentation CRDC 5 Part 2 10ºC 5 min Distill water ( (control) t l) 5 ppm 5 sub-replicates in 4 replicates 0 ppm 15 ppm Result and discussion 25ºC Percentage of weight loss of lime after treated with chitosan and then stored at 25ºC for 4 weeks. 3.5 3 2.5 % weight loss 2 control 1.5 5 ppm 1 % 10 ppm 0.5 15 ppm 0 1 2 3 4 weeks 7
21/07/54 Oral Presentation CRDC 5 Result and discussion 25ºC Percentage of lime disease after treated with chitosan and then stored at 25ºC for 4 weeks. 50 40 lime desease 30 control 20 5 ppm pp % of 10 ppm 10 15 ppm 0 1 2 3 4 weeks 10ºC Result and discussion 10ºC Percentage of weight loss of lime after treated with chitosan and then stored at 10ºC for 6 weeks. 5.00 4.00 % weight loss 3.00 control 2.00 5 ppm % 1.00 10 ppm 15 ppm 0.00 1 2 3 4 5 6 weeks 8
21/07/54 Oral Presentation CRDC 5 Result and discussion It maintained on the strawberries during 12 days at 7ºC (Devlieghere et al 2004) (Devlieghere et al., 2004) 2% chitosan significantly reduced the disease incidence and the lesion diameter in navel orange fruit compared with control fruit. (Kaifang Zeng et al., 2010) Fresh-cut strawberry which were dipped in 1.0% chitosan solution had less weight loss (1.19%), than dipped in control (2.04%) at 2ºC for 10 days and significantly different. (Sirisopha et al.,2005) 10 ppm chitosan solution is the best concentration that 25ºC 5 C can reduce fungal decay and delay weight loss 5 ppm chitosan treatment showed the best result in 10°C delay weight loss However, both hue and light values didn’t show any significant difference among all treatments. 9
21/07/54 Oral Presentation CRDC 5 Chitosan can be used for prolonging the storage life Chitosan can be used for prolonging the storage life and preventing loss of the products after harvest. Acknowledgements 10
21/07/54 Oral Presentation CRDC 5 Dr.Teerada Wangsomboondee Assist.Prof. Dr.Kanogwan Seraypheap Assist.Prof. Dr.Rath Pichyangkura Thank you Thank you 11
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