iGESM: i ntelligent G ene)cally E ngineered S lot M achine 1 Our - PowerPoint PPT Presentation

iGESM: i ntelligent G ene)cally E ngineered S lot M achine 1

Our aim Automa0c device for random recombina)on Inspired by the adap)ve immune system 2

Inspired by nature Random recombina)on of gene segments (V, D, J) Billions of an)bodies 3

iGESM‐project Recreate this system in bacteria 1. Random recombina)on between modules 2. Stops aEer produc)ve recombina)on COUNTLESS combina)ons & each cell ONE product 4

Site‐specific recombina0on Inverted repeats Direct repeats INVERSION DELETION 5

Gin recombinase Bacteriophage Mu Inver)ble G‐segment Gin recombina)on depends on recombina)onal enhancer! 6

Gin equa0on + = 7

Modular design A‐modules B‐modules Periplasm (AmiC) Green (GFP) Nucleoid (HU) Red (mRFP) Cell poles (GroES) Blue (CFP) 8

The recombinator construct 9

A‐modules 10

B‐modules 11

Automa0c shut off 12

Expected outcome 13

Results 14

Workflow 15

Workflow you are only limited by your imagina)on !!! 16

High diversity out of a few modules Shuts off aEer produc0ve recombina)on Many applica)ons 17

Team 18