iGEM Final al Jam ambore ree 11/05/201 05/2011
The he Dev evic ice Mining, People and the Environment, 03/12/2009 2011 World Championship Jamboree 2
The he Dev evic ice Importance to quantify mercury contained in water 2011 World Championship Jamboree 3
The he Dev evic ice • Meeting with a Chemical Industry Partner rtnership ship wit ith Industrial Needs: • Precise quantification (ECD: 50µg/L) • Reliable technics 2011 World Championship Jamboree 4
The he Dev evic ice State of the art atomic absorption spectrometry 2011 World Championship Jamboree 5
The he Dev evic ice Easy to handle Cheap 2011 World Championship Jamboree 6
The he Dev evic ice • Specifications : Single bacterial strain Comparative measurement Visual result 2011 World Championship Jamboree 7
The he Dev evic ice • Single Bacterial Strain 2011 World Championship Jamboree 8
The he Dev evic ice • Comparative Measurement – The reference molecule IPTG 2011 World Championship Jamboree 9
The he Dev evic ice • Comparative Measurement – Emergence of 2 bacterial behaviors : 2011 World Championship Jamboree 10
The he Dev evic ice • Comparative Measurement – Emergence of 2 bacterial behaviors : 2011 World Championship Jamboree 11
The he Dev evic ice • Visual Result – At the boundary both senders and receivers interact: 2011 World Championship Jamboree 12
The he Gen enet etic ic Net etwork rk 2-way Switch ch Colorat oration ion Power wer Button 2011 World Championship Jamboree 13
The he Gen enet etic ic Net etwork work • 3-level Genetic Network 2011 World Championship Jamboree 14
The he Gen enet etic ic Net etwork work • 2-way Switch Switch between 2 pathways 2011 World Championship Jamboree 15
The he Gen enet etic ic Net etwork work • 2-way Switch coupled with Quorum Sensing 1 Pathway 1 Behavior 2011 World Championship Jamboree 16
The he Gen enet etic ic Net etwork work • 2-way Switch coupled with Quorum Sensing 2011 World Championship Jamboree 17
The he Gen enet etic ic Net etwork work • 2-way Switch coupled with Quorum Sensing 2011 World Championship Jamboree 18
Mod odel eling ing an and d Exp xper erim iment ents 2-way Switch ch Colorat oration ion Power wer Button 2011 World Championship Jamboree 19
Modeling eling AND Exp xper eriments iments 2011 World Championship Jamboree 20
Mod odel eling ing AND Exp xper erim imen ents • 2-way Switch 2011 World Championship Jamboree 21
Mod odel eling ing AND Exp xper erim imen ents • 2-way Switch ODEs 2011 World Championship Jamboree 22
Mod odel eling ing AND Exp xper erim imen ents • Evolution of TetR concentration (1mM IPTG) 2011 World Championship Jamboree 23
Mod odel eling ing AND Exp xper erim imen ents • Biological Results 2011 World Championship Jamboree 24
Mod odel eling ing AND Exp xper erim imen ents • Evolution of GFP fluorescence (1mM IPTG) 2011 World Championship Jamboree 25
Mod odel eling ing AND Exp xper erim imen ents • Biological bistability IPTG aTc 2011 World Championship Jamboree 26
Mod odel eling ing AND Exp xper erim imen ents • Biological bistability 2011 World Championship Jamboree 27
Mod odel eling ing AND Exp xper erim imen ents • Biological bistability 2011 World Championship Jamboree 28
Mod odel eling ing AND Exp xper erim imen ents • Stochastic study of bistability 2011 World Championship Jamboree 29
Modeling eling AND Exp xper eriments iments • Quorum Sensing 2011 World Championship Jamboree 30
Modeling eling AND Exp xper eriments iments 2011 World Championship Jamboree 31
Modeling eling AND Exp xper eriments iments • Result on the plate aTc=50 ng/mL aTc=150 ng/mL 2011 World Championship Jamboree 32
Modeling eling AND Exp xper eriments iments • Result on the plate 2011 World Championship Jamboree 33
Modeling eling AND Exp xper eriments iments • Power Button 2011 World Championship Jamboree 34
Modeling eling AND Exp xper eriments iments • Post-transcriptional network 2011 World Championship Jamboree 35
Modeling eling AND Exp xper eriments iments • Post-transcriptional network 2011 World Championship Jamboree 36
Modeling eling AND Exp xper eriments iments • GFP fluorescence obtained with fha Counts Counts background fha rbs GFP Fluorescence Fluorescence GFP 2011 World Championship Jamboree 37
Modeling eling AND Exp xper eriments iments • The power switch using RsmA & rsm Y 350 Autofluorescence Fha 280 Fha + RsmA Counts 210 Fha + RsmA + rsm Y 140 70 0 10 0 10 2 10 1 10 3 10 4 GFP fluorescence 2011 World Championship Jamboree 38
Modeling eling AND Exp xper eriments iments • Channels on the device 2011 World Championship Jamboree 39
Modeling eling AND Exp xper eriments iments • Channels on the device 2011 World Championship Jamboree 40
Modeling eling AND Exp xper eriments iments • Device specifications Response time 1 hour Sensitivity 10 -7 M (20µg/L) 10% precision 10 4 bacteria per channel 2011 World Championship Jamboree 41
Hum uman an Pra ract ctic ice Interdi erdisc sciplinari narity ty Promot omotion ion 2011 World Championship Jamboree 42
Huma man Pra ractice tice • Synthetic Biology An interdisciplinary approach of living systems ! 2011 World Championship Jamboree 43
Huma man Pra ractice tice • How to improve the interaction between Biologists & Modelers ? 2011 World Championship Jamboree 44
Huma man Pra ractice tice 2011 World Championship Jamboree 45
Huma man Pra ractice tice 2011 World Championship Jamboree 46
Huma man Pra ractice tice • Test organization (With Flyers) (Without Flyers ) 2011 World Championship Jamboree 47
Huma man Pra ractice tice • Test results 10 8 Average Score (/10) 6 4 2 0 Group A (With Flyers) Group B (Without Flyers) 2011 World Championship Jamboree 48
Huma man Pra ractice tice • Test results Did the flyers help you you to better er understand rstand the questi tion ons? ? 16 % Negative 16 84 % Positive 84 2011 World Championship Jamboree 49
Huma man Pra ractice tice • Test results Did the flyers help you you to better er interact act with your ur partne ner? ? 38 % Negative 38 62 % Positive 62 2011 World Championship Jamboree 50
Huma man Pra ractice tice • Promoting Synthetic Biology – Conferences iGEM Grenoble 2011 at MINATEC iGEM Grenoble 2011 at INRIA – Media Attention (GreNews/A savoir/Radio) 2011 World Championship Jamboree 51
Conc nclusion lusion • Major achievements Innovative mercury biosensor Industrial interest ! Proof of concept of 3 modules: Quorum sensing Power button 2 Way-Switch 19 biobricks added. Models giving all necessary specifications of the device. New iGEM flyers for biologists & modelers. iGEM Grenoble 2012 is launched! 2011 World Championship Jamboree 52
Acknowledg knowledgments ments • Instructors • Advisor - Hans Geiselmann - Stéphane Pinhal - Franz Bruckert - Marianne Weidenhaupt - Hidde De Jong - Delphine Ropers - Robert Baptist 2011 World Championship Jamboree 53
Acknowledg knowledgments ments • Sponsors 2011 World Championship Jamboree 54
Acknowledg knowledgments ments • Sponsors • « CEA- Direction des Sciences du vivant » • « CEA- Direction des Sciences de la Matière » • « CEA-Direction de la Recherche Technologique » • Programme Transversal Nanotoxicologie • Programme Transversal Technologies pour la Santé • Programme transversal Nanosciences 2011 World Championship Jamboree 55
Thank you for your attention Questions ? 2011 World Championship Jamboree 56
Tr Tran ansla slational tional re regu gulation lation Infl fluence uence of f a a tran anslat ation ional al regulat atio ion n system m on the he switch given IPTG gradient nt with an aTc concentration of 10−6 M Low TetR concen entr tratio ation n : S Switch h at at lower er concent centra ration ion of IPTG (red curve) e). . 2011 World Championship Jamboree 58
Tr Tran ansla slational tional re regu gulation lation 2011 World Championship Jamboree 59
Tr Tran ansla slational tional re regu gulation lation pVLT31 T31-rsmA rsmA or • empty ty No growth th • difference rence Infl fluence uence of f RsmA product ction ion on the growth of f E c coli DH5 α ? 2011 World Championship Jamboree 60
Tr Tran ansla slational tional re regu gulation lation mag operon on leader er sequence uence Fha1 ha1 gene leader er sequence uence Construc ructs s used to charact aracter erise se rsmY sRNA sequence uence RsmA system em 2011 World Championship Jamboree 61
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