History SensoQuest SensoQuest History • Biotron Biotron GmbH was GmbH was founded founded in 1985 in 1985 by by • Jö örg Schrader, rg Schrader, now now CEO of SensoQuest CEO of SensoQuest J • The The main main business business was: was: • Production of thermocyclers Production of thermocyclers for for Biometra Biometra • 1993 1993 Whatman Whatman bought bought Biometra Biometra • • 1997 1997 Whatman Whatman- -Biometra Biometra bought bought Biotron Biotron • • 2001 2001 – – 05 05 Development Development of Labcycler of Labcycler • • 2005: 2005: Foundation Foundation of of SensoQuest GmbH SensoQuest GmbH •
Conception of the of the Conception Labcycler Labcycler • Long life Long life • • High High quality quality • • Modern and solid Modern and solid design design • • Easy Easy handling handling • • Touchscreen Touchscreen • • High High speed speed • • Low Low energy energy consumption consumption • • Low Low noise noise in the in the laboratory laboratory • • Universal Universal block block- -changing changing •
High Quality Quality, Long Life, , Long Life, High Modern & Solid Design Modern & Solid Design • Materials: Gold, Materials: Gold, Silver Silver, , Copper Copper & Aluminium & Aluminium • • Aluminium front Aluminium front plate plate, gold , gold coated coated silver silver blocks blocks, , • copper lids lids copper 6 seperately seperately controlled controlled peltier peltier elements elements of the of the 6 • • newest generation generation newest • Blocks Blocks with with self self calibration calibration temperature temperature • measuring measuring • Therfore Therfore a a tube tube thermistor thermistor is is not not necessary necessary for for • calibrating calibrating • Life Time: Life Time: More More then then 600,000 600,000 cycles cycles are are tested tested • • 20 20 years years life time in a life time in a standard standard laboratory laboratory •
Display & Keyboard Display & Keyboard • A modern Touchscreen A modern Touchscreen • • TFT TFT ¼ ¼ VGA VGA iluminated iluminated colour colour • • 76,800 76,800 pixel pixel, 5.7 , 5.7“ “diagonal diagonal • • Finger Finger tapping tapping or or Touch Touch pen pen tapping tapping • • Languages Languages English and German English and German • • Alphanumeric Alphanumeric function function keys keys • • Easy Easy to to use use software software with with selfexplaning selfexplaning icons icons • • Context Context- -sensitive sensitive help help function function • • Graphic Graphic monitoring monitoring of PCR of PCR process process •
Display & Keyboard Display & Keyboard Start window Thermoblock 96
Display & Keyboard Display & Keyboard Start window Triple Block 3 x 21 [0.2 ml tubes]
Automatic Lid Automatic Lid • Heated Heated lid lid • • Fully Fully programmable programmable with with the the touch touch screen screen • • Controlled Controlled by by an an electric electric motor motor • • Power 200 Watt Power 200 Watt • ° C/s rate 1 ° • Fast: Fast: Heating Heating rate 1 C/s • • Lid Lid can can be be deactivated deactivated • • Preheating Preheating can can be be deactivated deactivated • • Pressure Pressure 30 to 120 Newton (N) 30 to 120 Newton (N) • • E.g E.g.: 30 .: 30- -60 N 60 N for for single single tubes, 60 to 90 N tubes, 60 to 90 N for for • stripes, 90 to 120 N , 90 to 120 N for for microtiterplates microtiterplates stripes • Hotstart Hotstart- -procedure procedure possible possible • • Pause: Manual and Pause: Manual and programmable programmable •
Lid Automatic Lid Automatic Lid Unbolt handling key
Thermoblocks Thermoblocks • Thermoblock 48, 96, and 384 Thermoblock 48, 96, and 384 • Electroformed gold gold plated plated silver silver Electroformed Thermal conductivity conductivity 429 W/mK 429 W/mK Thermal ° C/s, ° C/s rate 4.2 ° rate 3.6 ° Heating rate 4.2 C/s, cooling cooling rate 3.6 C/s Heating ° C 0.01 ° • Control Control accuracy accuracy ± ± 0.01 C • • 6 6 seperately seperately controlled controlled peltier peltier elements elements of the of the • newest generation generation newest ° C to 99 ° C 5 ° C to 99 ° • Temperature Temperature – – 5 C • • Blocks Blocks with with self self calibration calibration temperature temperature measuring measuring • • • ° C at 55 ° C, 0.25 ° C at 55 ° • Uniformity Uniformity ± ± 0.25 C, ± ± 0.4 ° C at 95 ° C 0.4 ° C at 95 ° C • • Ramp rate 0.001 Ramp rate 0.001 ° ° C/s to 5.0 C/s to 5.0 ° ° C/s C/s • ± 9.99 ° C • De(In)crements De(In)crements: Temperature : Temperature ± 9.99 ° C • Time ± Time ± 99.99 seconds 99.99 seconds
Thermoblock 48 Thermoblock 48 48 Wells, 8 zone gradient, 0.5 ml tubes
Thermoblock 96 Thermoblock 96 96 Wells, 12 zone gradient, 0.2 ml tubes or microtiterplates
Thermoblock 96 Thermoblock 96 Detail picture of the electroformed gold plated silver
Thermoblock 384 Thermoblock 384 384 Wells, 24 zone gradient, microtiterplates The unique 384 well silver block
Triple Block 3 x 21 Triple Block 3 x 21 • Material: Material: Anodised Anodised aluminium aluminium • • Thermal Thermal conductivity conductivity 209 W/mK 209 W/mK • ° C/s, ° C/s rate 2.5 ° rate 2.2 ° • Heating Heating rate 2.5 C/s, cooling cooling rate 2.2 C/s • • 3 independent PCR 3 independent PCR runs runs • • 3 x 21 wells 3 x 21 wells for for 0.2 ml tubes 0.2 ml tubes • • Minimum Minimum volume volume 10 10 µ µl l • • Protection Protection against against condensation condensation by by 3 passive 3 passive lids lids • • Seperate Seperate and parallel and parallel monitoring monitoring of all of all blocks blocks •
Triple Block 3 x 21 Triple Block 3 x 21 Without passive lids
Triple Block 3 x 21 Triple Block 3 x 21 With one passive lid
Quick Block Changing Quick Block Changing System System Picture 1
Quick Block Changing Quick Block Changing System System Picture 2
Quick Block Changing Quick Block Changing System System Picture 3
Quick Block Changing Quick Block Changing System System Picture 4
Quick Block Changing Quick Block Changing System System Picture 5
Auto Re- -Start Start Function Function Auto Re Line Voltage Line Voltage 85 V to 265 85 V to 265 without without switching switching, 50 to 60 Hz , 50 to 60 Hz • • Black Out during during Black Out elongation cycle cycle elongation 72 ° ° C, step 72 C, automatic automatic step Re e- -S St ta ar rt t at the last at the last R ° C 95 ° denaturation step denaturation step 95 C to inhibite inhibite unspecific unspecific to annealing annealing Restart at the last denaturing denaturing cycle cycle inhibite inhibite unspecific unspecific products products Restart at the last • •
User interface interface User • Alphanumeric Alphanumeric and and function function keys keys in the in the touchscreen touchscreen • Special Special characters characters at at special special • • Figures Figures at at special special and and numeric numeric silicone silicone keys keys •
Programm Administration Programm Administration • More More than than 680 680 programmes programmes • Classification Classification in in folder folder, , person person, and , and group group, up to 4 , up to 4 levels levels •
Programm Administration Programm Administration • Copy Copy function function via via copy copy and and paste paste • Intersystem Intersystem copy copy function function between between two two devices devices also via also via • copy and copy and paste paste • Interfaces: 2* RS232 Interfaces: 2* RS232 for for software software- -updates updates • Software Software updates updates are are free free and and always always compatible compatible with with old old devices devices •
Programme INFO Programme INFO • Runtime Runtime • • Display of the Display of the remaining remaining runtime runtime of the of the active active process process (PCR) (PCR) •
Standard PCR Standard PCR 1. Initial 1. Initial Denaturating Denaturating Step Step ° C 98 ° • 1- -5 min, 95 5 min, 95- -98 C • 1 2. Denaturing Denaturing Step Step 2. 95 ° ° C • • 0.5- 0.5 -2 min, 94 2 min, 94- -95 C 3. Primer Annealing Step Primer Annealing Step 3. ° C ° C) 2 min, 5 ° . 58 ° • 0.5- -2 min, 5 C lower lower than than melting melting temperature temperature ( (e.g e.g. 58 C) • 0.5 4. Extension Step Extension Step 4. ° C 75 ° • 1 min for for each each 1000 bp 1000 bp using using Taq Taq- -polymerase, 70 polymerase, 70- -75 C • 1 min 5. Number 5. Number of of Cycles Cycles • 25- -35 (max. 40) 35 (max. 40) • 25 6. Final Extension Step Final Extension Step 6. 15 min, 72 ° ° C • • 5- 5 -15 min, 72 C 7. Storage Storage after after PCR PCR 7. ∞ ), 4 ° C ( ∞ 8 ° • Endless ( ), 4- -8 C • Endless
Recommend
More recommend
