1434/07/09 LOGO Chemical Engineering Department Ferdowsi university of mashhad diesel oil biodegradation in bioreactors Maryam sadat Atyabi Dr . Gheshlaghi 1 Contents 1. . Introduction Introduction 2. . Bioreactors Bioreactors 3. baffled roller bioreactor . baffled roller bioreactor 2 1
1434/07/09 Introduction Accidental release of petroleum products into the environment[5] benzene, toluene, ethylbenzene and xylene (BTEX) toxicity of DF Cancer 3 Introduction Several methods for the remediation of petroleum contaminated sites[5] Mechanical (e.g. oil – water separators) Chemical (using chemicals dispersants such as surfactants) Biological (based on bioremediation) 4 2
1434/07/09 Introduction Arrar Arrar et al et al 2007 2007 jet-fluidized bed reactor[1] Kleijntjens Kleijntjens et al et al 1991 1991 continuously stirred bioreactor[4] Nikakhtari Nikakhtari et al et al 2009 2009 baffled roller bioreactor[1] Whang et al Whang et al 2008 2008 batch reactors[3] 5 Bioreactors The efficiency of a bioreactor[6] the establishment of an ideal environment the development of a technology for control of the conditions in the reactor basic physical modes of providing the microorganisms fixed-film support suspended growth in solution 6 3
1434/07/09 baffled roller bioreactor 7 [3] Microorganism and medium Commercial and donated microorganisms from different sources[3] Alabaster Corp Pasadena, TX Bethlehem Bioscience Inc The indigenous microorganisms isolated from a contaminated soil of an industrial site 8 4
1434/07/09 MICROBIAL CULTURE To find the indigenous bacteria best microbial isolated from soil culture from a contaminated industrial site[3] different microbial cultures 9 MICROBIAL CULTURE 10 [3] 5
1434/07/09 microorganisms Rhodococcus Pseudomonas erythropolis aeruginosa microorganisms microorganisms Achromobacter Rhodococcus xylosoxidans fascians 11 Cell growth model Did not show any substrate Monod equation inhibition[3] Runge-Kutta method , experimental data 12 6
1434/07/09 surfactant (SDS) Profile of diesel oil concentrations in the baffled roller bioreactor with and without surfactant (SDS) [3]. 13 Effect of temperature Biodegradation of 1000 mg L− 1 diesel oil in the baffled roller bioreactors at different temperatures [3]. 14 7
1434/07/09 Modeling results 15 [3] Comparison of bioreactors diesel oil consumption in various roller bioreactor configurations: Biomass growth in various roller bioreactor configurations: control, baffled, and bead mill [2],[3]. control, baffled, and bead mill [2],[3]. 16 8
1434/07/09 Comparison of bioreactors baffled bioreactor faster biodegradation half the working of diesel in both volume of bead Baffled and bead mill mill bioreactor bioreactors than the control bioreactor[2],[3] 17 Comparison of different scales Biodegradation of diesel oil in 3 different scales of roller baffled bioreactors[3] 18 9
1434/07/09 REFERENCES 1. Mixtli Campos-Pineda, Karim Acuna-Askar,Jesus Alberto Martinez-Guel, Marcela Mas-Trevino, Rolando Tijerina-Menchaca, Luz Maria Martinez, Marcelo Videa & Roberto Parra-Saldivar.(2011). Time and cost efficient biodegradation of diesel in a continuous-upflow packed bed biofilm reactor and effect of surfactant GAELE. 2. Hossein Nikakhtari, Wanning Song, Pardeep Kumar, Mehdi Nemati, Gordon A. Hill.(2010). Scale up of diesel oil biodegradation in a baffled roller bioreactor. 3. Hossein Nikakhtari,Pardeep Kumar, Mehdi Nemati and Gordon A. Hill.(2008). Biodegradation of diesel oil in a baffled roller bioreactor. 19 REFERENCES 4. Edelvio B. Gomes, Ricardo F. Silva, Alexandre S. Rosado, Nei Pereira Jr.(2010). Biotreatment of diesel waste by sequencing batch bioreactor operation mode (SBR). 5. A. Lohi, M. Alvarez Cuenca, G. Anania, S.R. Upreti,L.Wan. (2007). Biodegradation of diesel fuel-contaminated wastewater using a three-phase fluidized bed reactor. 6. Violeta Bravo&Wolfgang Spyra & René Antaño - López .(2009). Biodegradation of High Concentrations of Benzene and Diesel in a Fixed- Film Reactor. 20 10
1434/07/09 LOGO 21 11
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