Customer Handoff & Final Project Documentation P18082 - PowerPoint PPT Presentation

Customer Handoff & Final Project Documentation P18082 Electrical Bioreactor

Agenda 1. Team Vision Emily 6. 1. Functional Demo Materials 2. Test Results Summary Jon+Shena+Dillon a. Mechanical Luc b. Electrical Jon a. Test Plan 7: Electrical Requirement Verification 2. Conclusion All 7. Test b. Test Plan 8: Full-Run Electrical Bioreactor Test 3. Problem Tracking Emily 4. Risk Assessment Dillon 5. Final Project Documentation a. Design Schematics and Drawing Files Luc b. Bill of Materials Dillon c. Operators Manual All d. Software Jon e. Recommendations for Future Work Emily

1. Team Vision 1. Rerun Test Plan 8 with corrected voltage values 2. Complete test plan result documentations 3. Finalize bioreactor unit redesign a. Complete machining and assembly of bioreactor for Imagine b. Complete electrical wiring for Final Presentation 4. Complete all documentation and manual for customer handoff following finals 5. Have successful presentations a. IAB Meeting b. Lighting Talks c. Imagine RIT

1. Team Vision - Schedule

1. Team Vision - Schedule Continued

1. Team Vision - Schedule Continued ● All tasks outlined in the schedule were completed for phase 4 except for the following ○ 4.12 Customer Handoff ■ WIll occur following finals week with Prof Bailey ○ 4.16 Review with Guide Research Paper ■ Did not need to occur ○ 4.18 Complete Edge Website ■ Edge will be updated following final MSD review with guide and customer ○ 4.19 Clean Workspace ■ Workspace will be cleaned following manufacturing and assembly of additional carbon electrodes and 6-well plates

Test Plan Summary

2. Test Plan 7: Electrical Requirement Verification Test

2. Test Plan 7: Electrical Requirement Verification Test

2. Test Plan 7: Electrical Requirement Verification Test (Customer Requirements) Apply a static or cyclic voltage across the culture for up to 3 50% CR2.1 1 weeks Passed CR2.2 3 Controllable and adjustable stimulation during the experiment Passed CR3.1 2 Verification of correctly applied voltage Passed CR4.1 1 Safe for student population usage

2. Test Plan 7: Electrical Requirement Verification Test (Engineering Requirements) Unit of Ideal Actual Engr. Requirement (metric) Marginal Value Test Result Measure Value Value <5 0 - 0.5 Applies a voltage V 0 - 5 0 - 0.5 +/- 0.05 +/- 0.05 +0.015V Measures the applied voltage V +/- <0.015 +/- 0 -0.007V Quickly adjustable applied voltage min 1 - 3 1 <0.5 <0.5 No Passed Software usage straightforward - Minimal Questions Questions Shock hazard during normal operation including moving for Passed - - - media exchange or viewing on microscope

2. Test Plan 8: Full-Run Electrical Bioreactor Test

2. Test Plan 8: Full-Run Electrical Bioreactor Test Test Well 1 on Day 2: Cells look healthy Test Well 2 on Day 2: Cells look healthy and thriving and thriving

2. Test Plan 8: Full-Run Electrical Bioreactor Test Control Well 1 on Day 2: Cells look healthy Control Well 2 on Day 2: Cells look healthy and thriving and thriving

2. Test Plan 8: Full-Run Electrical Bioreactor Test

3. Problem Tracking

3. Problem Tracking - Continued 3. Unknown reactor resistance ● Wrote new code for LabVIEW and Arduino ● Confirmed current output values were in desired range 4. Unknown reactor current ● Wrote new code for LabVIEW and Arduino ● Confirmed current output values were in desired range 9. Carbon electrode adhesion process is not sterile ● Viability of cells was not affecting by carbon electrode adhesion and no further actions needed to be taken

3. Problem Tracking - Continued 10. Spring-latch holding in well-plate is inefficient ● Redesigned spring-latch and reconfigured location ● More ergonomic spring-latch implemented in redesign of bioreactor unit 11. Arduino housing hinge is rigid ● Research was conducted to find a hinge with better hinge properties ● Less rigid hinge was implemented in the new design 12. Electrical Bioreactor is heavy compared to other biological items ● Lighter materials of Polycarbonate and Acetal Delrin replaced Aluminum in redesign of bioreactor unit

3. Problem Tracking - Continued 10. Voltage too high for first Test Plan 8 trials ● Lower voltage values need to be considered ● Recommended 0.2 - 0.5 V range following electrical tests 11. Cells in Test Plan 8 were dying ● Voltage ranges in 11 was not implemented in rerun of Test Plan 8 ● Recommended MAX voltage value of 0.5 V to sustain cell growth

4. Risk Assessment ● Performed a final review of risk items ● Determined whether the risks were avoided ● Most risks were addressed and reduced ○ Risk #7: Cell death due to lack of space -- Electrodes were cut so that they could be spaced at maximum effective distance, which would allow the maximum space for seeding cells. However, future users may be required to determine a means of slowing cell growth through adjustments in the cell media. ○ Risk #8: Screws/threaded holes become stripped -- The final product was fabricated out of polycarbonate and acetal delrin in order to reduce the system weight and avoid the possibility of drops which result in stripping the fasteners. However, should accidents occur, a gluing or bonding material is recommended to make fixes.

4. Risk Assessment

5a. Design Documentation Arduino Housing Front Wall

5a. Design Documentation Arduino Housing Side Walls

5a. Design Documentation Arduino Housing Lid

5a. Design Documentation Back Plate

5a. Design Documentation Base Plate

5a. Design Documentation Bracket

5a. Design Documentation Electrical Connection Plate

5a. Design Documentation Latch

5a. Design Documentation Wire Plate

5a. Design Documentation Lift Tab

5b. Bill of Materials ● No items are planned for or awaiting Total Budget $1,000.00 delivery. ● We went over our original budget of Current Expenses $552.02 $500.00 by $52.02. ● Biological items drove up the cost of the project. Current Budget $447.98 ● Should only the physical system be required, then costs will be reduced by approximately half. Projected Costs $0.00 ● In addition, the final design requires fewer components, which should drop the cost even more. Estimated Final $447.98 Budget

Item Vendor Catalog # Individual Cost White Delrin 5b. Bill of Materials Acetal Bar, McMaster-Carr 8739K62 $44.32 1-1/4" Thick, 4" Wide, 1' Long ● An example of some of the items we Clear Polycarbonate recommend should Dr. Bailey desire to Sheet, ½” Thick, McMaster-Carr 8574K32 $32.34 12” Wide, 12” build another system. Long Off-White Nylon Pan Head Screws Phillips McMaster-Carr 93135A454 $7.41 ● Should be used in conjunction with 1/4"-20 Thread, 3/4" Long manufacturing and assembly instructions. Off-White Nylon Pan Head Screws Phillips McMaster-Carr 93135A456 $7.65 1/4"-20 Thread, ● The plan is to drop off a list of materials 1-1/2" Long and where to purchase them at the Adjustable-Fricti customer hand-off. on Surface-Mount McMaster-Carr 1791A44 $6.92 Hinge, Black Acetal Plastic, 7 in.-lbs. Torque

5c. Operators Manual We would like to navigate to the GoogleDrive to open the Operators Manual for demonstration

5e. Recommendations for Future Work ● Mechanical ○ Continue to look for ergonomic and system improvements of the bioreactor unit ■ Materials ■ Arduino housing hinge ■ Well plate spring-latch ● Electrical ○ Addition of cyclic waveform to be included for student selection ■ With user variable frequency ● Biological ○ Allow class to choose preferred cell line for electrical testing ○ Determine effective testing voltages based on cell line

7. Conclusion The end product, following the senior design process from Problem Definition through Integrated System Build & Test, is a functional electrical bioreactor that the Advanced Cell Culture Techniques course can utilize as a tool to study the effects an electrical stimulus will have on tissue engineering cells. We would like to personally thank Dr. Jennifer Bailey and Dr. Vinay Abhyankar for their guidance and insight of cell culture and tissue engineering. In addition, we appreciate the access to their research laboratories. The Kate Gleason College of Engineering, the Biomedical Engineering Department, Mechanical Engineering Machine Shop at RIT, and Robotics Lab are to be thanked: including Dr. Steven Day , Dr. Jason Kolodziej , and Mr. Jan Maneti. A special thank you to our guide, Michael Zona, for his patience, guidance, and knowledge on the multidisciplinary senior design process and engineering requirements.

Misc. Info

2. Test Plan 8: Full-Run Electrical Bioreactor Test Cell Initial Check Secondary Check Viability Test (4/27/18) Living Cells/uL Dead Cells/uL Living Cells/uL Dead Cells/uL Test 1 3 7.5 8 20 0 0 1 2.5 Test 2 3 7.5 1 2.5 2 5 2 5 Control 1 12 30 1 2.5 29 72.5 10 25 Control 2 114 285 1 2.5 257 642.5 0 0 Initial check was performed on Friday afternoon (4/27) and the secondary check was performed several hours later. The secondary check was performed to validate the numbers observed in the initial check.