Complete Portfolio of Biopharma Solutions Sample Sample Data - PowerPoint PPT Presentation

Complete Portfolio of Biopharma Solutions Sample Sample Data Preparation Analysis Interpretation Unregulated Regulated

UltiMate + TSQ Vantage Regulated quantitation of small molecules, peptides, and biologics • Highest sensitivity, lowest noise for peptide mapping of biologics • Consistency and ruggedness for high throughput discovery applications

QuickQuan and QuickCalc Automate the entire triple quad drug discovery workflow • Industry’s only fully automated infusion optimization process • HT data acquisition, processing and reporting • Multiple calculation modules: PK, Hepatic Clearance, Permeability

MetQuest Software Automated Quan/Qual Metabolic Screening • Integrated workflow for Orbitrap-based instruments • Data acquisition with generic method • Unbiased high-resolution full-scan data

TSQ Module for Watson LIMS Simplifies daily GLP workflow • Integrated LC-MS/MS control • Chromatographic peak integration and re-integration inside of Watson • Single reporting package for LC-MS/MS data • Storage of raw data inside of Watson LIMS

Transcend for HTS Applications • Improved selectivity with reduced costs and sample preparation • Rapid sample collection with chromatographic separation • Triple quad and HRAM compatible

Laser Diode Thermal Desorption 125 125 125 125 125 125 120 120 120 120 120 120 115 115 115 115 115 115 110 110 110 110 110 110 105 105 105 105 105 105 • Rapid compound optimization and sample 100 100 100 100 100 100 95 95 95 95 95 95 90 90 90 90 90 90 analysis 85 85 85 85 85 85 80 80 80 80 80 80 75 75 75 75 75 75 Relative Abundance Relative Abundance Relative Abundance Relative Abundance Relative Abundance Relative Abundance 70 70 70 70 70 70 65 65 65 65 65 65 • Ten 96-well plates in less than 3 hours 60 60 60 60 60 60 55 55 55 55 55 55 50 50 50 50 50 50 45 45 45 45 45 45 40 40 40 40 40 40 35 35 35 35 35 35 • No additional sample preparation 30 30 30 30 30 30 25 25 25 25 25 25 20 20 20 20 20 20 15 15 15 15 15 15 10 10 10 10 10 10 5 5 5 5 5 5 0 0 0 0 0 0 1.8 1.8 1.8 2.0 2.0 2.0 2.2 2.2 2.2 2.2 2.2 2.2 2.4 2.4 2.4 2.4 2.4 2.4 2.6 2.6 2.6 2.6 2.6 2.6 2.8 2.8 2.8 2.8 2.8 2.8 Time (min) Time (min) Time (min) Time (min) Time (min) Time (min)

UltiMate 3000 MAb Analysis Platform • Fully biocompatible • Dual ternary gradient pumps • Multifunctional autosampler with fraction collection and sample derivatization • Protein purification

Intact Monoclonal Antibody Analysis Using LC-MS Achieve ultimate confidence (c) 53.8 fmol Orbitrap, R= 7,500 53.8 fmol Normalized intensity (a) • Dionex monolithic columns for intact protein analysis 2,200 3,100 4,000 m/z (b) 5.38 fmol • Dionex UltiMate 3000 RSLCnano for (d) 5.38 fmol high chromatographic fidelity 10 0 Minutes • High mass accuracy, high-resolution mass spectrometric detection 2,200 3,100 4,000 m/z

Lys C MAb Digest Analysis by Capillary LC-MS/MS 1.5 Intensity × 10 7 • UHPLC for high 0 chromatographic 5 0 10 15 20 25 30 35 Minutes y6 100% fidelity and resolution V C+58 L L N+1 N F Y S G T A S V P R Y F N N+1 L L C+58 V V R P S A T G S 80% y2 MS/MS spectrum Relative Intensity • High-resolution MS for 60% y3 deamidation fast and accurate y6+1 b14 40% b14+1 protein identification b13 y9 b10-H2O b10 y5 20% y7 y10 y4 y8 b7 b12 b9 b6 b8 b11 y11 0% 0 250 500 750 1000 1250 1500 1750 m/z

EASY-nLC 1000 Effortless UHPLC • 1000 bar operating pressure • Dedicated solution • Market-leading ease of use • Integrated design, easy maintenance

EASY-nLC 1000 Xcalibur Control • Versatile MS data system • Wizard-style method development • 21 CFR Part 11 compliance

EASY-nLC 1000 Ready to run • Predefined tubing • MS-based QC • Instant trouble-free separation • Uncompromising performance

Pinpoint Quantitative Proteomics Software • Accelerates targeted protein quantitation in biological research • Facilitates the transition to targeted quantitation • Start-to-finish solution for biomarker ID and verification

End-to-end Bioanalysis Solutions • Best-in-class protein analysis columns • HPLC platforms to increase throughput and productivity • Global leader in high-resolution, accurate mass MS for ultimate confidence

Quantitation by MS HeavyPeptide AQUA Standards For relative and absolute quantitation of proteins in Proteomics and Biopharma

Better MS Sample Prep Pierce Graphite Spin Columns Purify and concentrate hydrophilic peptides in <10 minutes

Detergent Removal Products Better sample prep means better results New 96-well spin plates for high-throughput, or spin columns for 10µl-1mL samples

Capture and Enrich Kinases Enrichment kits with novel ATP- and ADP-based probes Covalently modify active sites of kinases, chaperones and metabolic enzymes