Biomass Bioaugmentation in a Single-Stage Nitritation-Anammox - PowerPoint PPT Presentation

UNIVERSITY OF ARIZONA Water & Energy Sustainable Technology Center HEADER TEXT Biomass Bioaugmentation in a Single-Stage Nitritation-Anammox Reactor for Mainstream Deammonification Jim Field 1 , Jeff Prevatt 2 , Guangbin Li 1 and Reyes Sierra 1 guangbinli@email.arizona.edu 1 , Department of Chemical and Environmental Engineering, University of Arizona 2 , Pima County Regional Wastewater Reclamation Department (PCRWRD) Funding Partners Award#1705671

UNIVERSITY OF ARIZONA The N Cycle Water & Energy Sustainable Technology Center HEADER TEXT Org-N assimilation mineralization N-fixation Conventional Nutrient N Removal Costs + NH 4 N 2 Nitrification: nitrification-denitrification AOB 64 g O 2 / mol N O 2 NOB e - - NO 2 e - O 2 Denitrification: - + 5 e - eq → 0.5 N 2 NO 3 40 g COD/ mol N - NO 3

UNIVERSITY OF ARIZONA The Missing Link In The N Cycle Water & Energy Sustainable Technology Center HEADER TEXT Anammox Org-N Anaerobic Ammonium Oxidation mineralization assimilation Simplified Stoichiometry N-fixation + NH 4 + NO 2 - N 2 dinitrogen gas nitrate ammonia Anammox Discovered in Late 1980’s in The Netherlands + NH 4 N 2 120 1,400 Scientific publications Full scale installation O 2 e - - NO 2 e - O 2 1995 2015 - NO 3 Year Lackner et al., 2014

UNIVERSITY OF ARIZONA Costs and Savings of Anammox Water & Energy Sustainable Technology Center HEADER TEXT Anammox Reaction (Detailed Stoichiometry) no O 2 needed for anammox no COD needed for anammox Anammox is combined with AOB as a technology 43% flow + NH 4 N 2 89% N (100% N) Anammox - NO 2 - 11% N NO 3 Nitritation AOB 57% flow O 2 57% less O 2 No COD

UNIVERSITY OF ARIZONA Moving-Bed Biofilm Reactor (MBBR) Water & Energy Sustainable Technology Center HEADER TEXT The ANITA Mox is based on developing an Anammox biofilm on a plastic carrier material in a Moving-Bed Biofilm Reactor (MBBR) A variant of the ANITA Mox process is to integrate suspended activated sludge (A/S) biomass in AOB the process to help catalyze the PN by AOB. (IFAS) Mixer Aeration - NO 2 Treated Effluent + NH 4 DO ANAMMOX + Media NH 4 N 2 - NO 2 Wastewater Vol. 145 L

UNIVERSITY OF ARIZONA MBBR Control and Operational Strategy Water & Energy Sustainable Technology Center HEADER TEXT pH-DO Control strategy AOB consume ALK vs. Anammox generate ALK Operational Plan Low DO for AOB & High DO for NOB Phase Purpose Influent Ammonium-N Upper limit I Startup (unstable influent quatity) Centrate 300-1600 ppm pH II Sidestream I: MBBR Centrate 1000 ppm Lower limit III Sidestream II: MBBR and IFAS Centrate 1000 ppm IV Mainstream: MBBR and IFAS Mainstream 40 ppm Air pH: upper limit = 7.2 and lower limit = 6.6

UNIVERSITY OF ARIZONA MBBR Performance Water & Energy Sustainable Technology Center HEADER TEXT Influent pH = 7.5 Temp. 86 F (30 degree C) Current Nitrogen Loading Rate (NLR): 1.0 g N/L reactor/day

UNIVERSITY OF ARIZONA Upflow Anaerobic Sludge Blanket (UASB) Water & Energy Sustainable Technology Center HEADER TEXT The UASB is based on Anammox biomass forming compact granular sludge with high specific activity. Treated Effluent Air N 2 Granule AOB: Ammonium oxidizing bacteria Sludge Bed AOB: Ammonium oxidizing archaea Recirculation Three-phase separator Centrate/Mainstream WW Aeration Vol. 65 L

UNIVERSITY OF ARIZONA UASB Control and Operational Strategy Water & Energy Sustainable Technology Center HEADER TEXT Operational Plan Phase Purpose Influent Ammonium-N Nitrite-N Total-N I Startup Synthetic 80-200 ppm 90-225 ppm 170-425 ppm II Sidestream I: Anammox Synthetic 200-480 ppm 225-540 ppm 425-1000 ppm III Sidestream II: PN/A Synthetic 1000 ppm 0 ppm 1000 ppm IV Sidestream III: PN/A Centrate 1000 ppm 0 ppm 1000 ppm V Mainstream: PN/A Mainstream 40 ppm 0 ppm 40 ppm Control strategy in Anammox Stage pH vs. Anammox Activity pH vs. Anammox Growth Normalized Anammox Activity (NAA, %) Effect of pH on the anammox activity of a granular enrichment Effect of pH on the anammox growth rate culture ( ● ) and a suspended enrichment culture ( ○ ) HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

UNIVERSITY OF ARIZONA UASB Performance Water & Energy Sustainable Technology Center HEADER TEXT UASB Other Operational Parameters Influent pH = 7.7 Temp. 86 F (30 degree C) HRT = 0.25 day SRT = 230 day ALK = 800 mg/L as CaCO 3 Nitrogen Loading Rate (NLR) NLR v = 2.8 g N/L reactor/day NLR = 0.5 g N/g VSS/day Performance Interruption DO interruption (> 6 mg/L) Loading Shock (TN > 2000 mg/L) pH interruption (> 8.0)

UNIVERSITY OF ARIZONA Water & Energy Sustainable Technology Center HEADER TEXT PARTNERS FUNDING Collaborative Research: WERF: GOALI ( ##1705674 ) Acknowledgement Prof. Jim Field Jeff Prevatt Prof. Reyes Sierra Prof. Mark Krzmarzick Steve King Jack Parker Jeffrey Bliznick Mallory Rae McMurray Joleen Maile Iwako Liftee Shiroma Jacob Smutzer Jesus Alejandra Fraijo Arce