aia
play

AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA - PowerPoint PPT Presentation

Nov 18, 2023 •342 likes •845 views

A SAI AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA Watch our animated movie which explains our project : http://2010.igem.org/Team:EPF_Lausanne/Humanpractices_Movie p2 p25 5 / p / p28 p2 p25 5 / p / p28 p2 p25 5 / p

  1. A SAI AIA , , TH THE PIN INK FORC RCE A GA INST M AL ARIA GAIN ALARIA

  2. Watch our animated movie which explains our project : http://2010.igem.org/Team:EPF_Lausanne/Humanpractices_Movie

  3. p2 p25 5 / p / p28

  5. p2 p25 5 / p / p28

  9. p2 p25 5 / p / p28

  10. p25 / p p2 / p28 Im Immun unotox otoxin in

  12. Yoshida et al, Molecular and Biochemical Prasitology, 2000

  13. pelB FLAG Tag Heavy chain linker Light chain Shiva HIS Tag Pore-forming Antibody Toxin fragment

  14. 1 st goal Find a way to deliver these proteins to the mosquito’s gut!

  15. ASAIA ADDRESS : mos osquito's ito's intestin estinal al tra ract SPECIAL CHARACTERISTIC : pink! k! ADDITIONAL INFO : speci ecific fic or origi gin RECENTLY FRIEND WITH : E. E.col oli

  16. Asaia is a commensal bacterium of mosquitoes Male terminal portion of mitgut Female terminal portion of mitgut Favia et al., PNAS 2007

  17. Horizontally Vertically

  18. Our project

  19. Find a way to deliver these proteins to the mosquito’s gut!

  20. 2 nd goal Establish Asaia as a new chassis for iGEM

  21. Asaia growth characterization pH5 Best conditions : 30°C, pH5

  23. Asaia GFP in Drosophila 3 hours after infection Observation of fluorescent Asaia in the Drosophila's gut

  27. Crotti et al., AEM Acetic acid bacteria, new emerging symbionts of insects, 2010

  28. Asaia origin Extracted from pHM2-GFP Plasmid Compatible with E. coli

  29. Asaia Asaia E. coli origin origin origin

  30.  Characterized Asaia growth  Characterized Asaia’s antibiotic resistance spectrum  Tested Asaia infection in Drosophila  Generated tech sheets for working with Asaia: growth, competent cells, transformation, and cloning

  32. Establish Asaia as a new chassis

  33. 3 rd goal Construct and characterize BioBricks

  39. Lysate Supernatant

  40. Construct and characterize BioBricks

  41. Human practices

  46. Express p25, p28, and the Immunotoxin in Asaia Test in vivo (Institute Pasteur) Introduce the engineered Asaia strain in the wild

  48. Acknowledgements

Recommend

More recommend