2019, Sublethal effects of clothianidin, a neonicotinoid insecticide - PowerPoint PPT Presentation

2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health Erica Brock, CFANS RAP student, Temo Balaxashvilli MAST student, and Vera Krischik. Assoc Prof Entomology

Understanding the partial contribution of pesticides to bee mortality and developing BMP to mitigate mortality, IPM is part of BMP

Neonicotinoid birds + bees: Fate of neonicotinoids + pathways of environmental contamination (Sanchez-Bayo 2014 Science)

Why are neonicotinoids so much more toxic to bees compared to other insecticides? • Receptors in bees not in mammals • Adjuvants increase toxicity • Alters behavior +foraging at sublethal doses • Water soluable • Binds with soil

2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health of the native, common bumblebee Bombus impatiens

2019, Sublethal effects of clothianidin, a neonicotinoid insecticide that alters bee behavior and chlorantraniliprole, a bee friendly insecticide, on movement, pollen consumption, reproduction, and hive health I participated in a study with bumble bees in the greenhouse in large tents. Bumbles were assigned to 3 treatments (n=8hives/trt); controls 0 ppb insecticide, clothianidin 20 ppb, and chlorantraniliprole 4ppm. Clothianidin provide in sugar syrup resulted in decreased movement, pollen consumption, colony weight, bee movement, and brood production. Chlorantraniliprole at 4ppm was no different than controls for all measurements of bee and colony health.

Measuring colony growth thru time

2018 field exp Bumble bees at 2 and 3 wks, clothianidin trts starts to decline at 2 wks, fungus 20 ppb clothianidin 4ppm chlorantraniliprole control

Bumblebee (8/trt) pollen consumption (g) over time clothianidin treatment eat less pollen over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control

Bumblebee (8/trt) sugar syrup consumption (g) over time clothianidin treatment eat similar amounts over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control Beehappy consumption 1400.00 1200.00 1000.00 800.00 600.00 400.00 200.00 0.00 2/19/2019 2/26/2019 3/5/2019 3/12/2019 3/15/2019 3/19/2019 N A C

Bumblebee (8/trt) colony weight (g) over time clothianidin treatment has less weight over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control Cumulative WT Change 400.00 350.00 300.00 250.00 200.00 150.00 100.00 50.00 0.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019 N A C

Bumblebee (8/trt) colony movement over time clothianidin treatment has less movement over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control Movement 25.00 20.00 15.00 10.00 5.00 0.00 3/5/19 3/8/19 3/12/19 3/15/2019 3/19/2019 N A C

Bumble bee (8/trt) full honey pots over time all treatments not different over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control Full honey pots 200.00 180.00 160.00 140.00 120.00 100.00 80.00 60.00 40.00 20.00 0.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019 N A C

Bumble bee (8/trt) total brood over time clothianidin treatment has less total brood over time n= blue, clothianidin, class neonicotinoid, highly oxic to bees A= chlorantraniliprole, a bee friendly insecticide c= control Total brood 120.00 100.00 80.00 60.00 40.00 20.00 0.00 2/19/2019 2/22/2019 2/26/2019 3/1/2019 3/5/2019 3/8/2019 3/12/2019 3/15/2019 3/19/2019 N A C

Bumblebee (8/trt) brood stage 1 over time clothianidin treatment has less stage 1 young brood over time n= blue, clothianidin, class neonicotinoid, highly toxic to bees A= orange, chlorantraniliprole, a bee friendly insecticide c= grey, control Brood stage 1 60.00 50.00 40.00 30.00 20.00 10.00 0.00 -10.00 N A C

Thanks to the CFANS RAP AP fellowship and the Krischik l lab for h r hosting m me to participate in bumbleb ebee ee resea earch