18 April 2016 Jan Pen Centre of Expertise for Plant Compounds - PowerPoint PPT Presentation

Extract Library 18 April 2016 Jan Pen Centre of Expertise for Plant Compounds

Background The Extract Library was set-up to provide a bridge between the huge biodiversity in plant compounds in Dutch horticultural crops and applications of those crops in high-value non-traditional markets

Market Diverse market segments with a common need for novel products Pharmaceuticals Agro(chemicals) Natural colours, flavours and fragrances Cosmetics & toiletry Herbal medicines Food supplements Feed additives Fine chemicals

Market need • Biodiversity plant compounds • Information on cultivars and plant material – Taxonomy, known compounds, (traditional) use, edibility, toxicity etc. • Reproducible supply • Cultivation without chemicals • Compliance with regulatory requirements

Extract Library

Crops • Huge diversity in plant types • Families: 205 • Genera: 927 See list • Species: 1,300 • Huge diversity in plant compounds • Estimate: 100,000 – 200,000 • Can be grown in The Netherlands (or elsewhere) • Controlled and reproducible cultivation • Reproducible composition and amount of plant compounds • No chemical crop protection products used • No false positives

Families & # of Genera

Plant materials – Plant materials: 2,245 – From one crop: 1-3 plant materials • Buds, beans, bulbs, branches, bark, cabbage, cress, Amount Plant available Materials exudate, flowers, fruits, pods, leaves, mushrooms, (g) (#) nuts, roots, seeds, wood, stems, sprouts, substrate, tubers and combinations thereof 0 35 < 50 53 – Maximally harvested: 250 g 50-100 154 – Used for extraction: 50 g 100-150 166 – Maximally available for e.g. follow-up R&D: 200 g 150-200 1,837 Total 2,245

Extracts – Compliant with industry and regulatory requirements – Crude extracts: 2,244 • Extraction solvent: 96.5% ethanol • Final solvent: app. 89% ethanol* • Concentration: 1 ml corresponds with 200 mg plant material (f.w.) – Cleaned extracts: 2,244 • Sugars, fatty acids, bio-polymers, salts, amino acids and tannins removed • Final solvent: app. 57% ethyl acetate, 23% ethanol, 20% water (v/v/v)* • Concentration: 1 ml corresponds with 200 mg plant material (f.w.) *Can be supplied in dry form

Extracts in plates – Nature and form suitable for screening by various industries – Crude extracts: 2,240 • Plate format: 96-deep well plates with removable tubes Cherry picking possible • Extracts per plate: 80 • Library (2,240 extracts): 28 plates • Content/well: 0.5 ml extract in app. 89% ethanol* Corresponds with 100 mg plant material (f.w.) – Cleaned extracts: 2,240 • Plate format: 384-deep well plates Fixed format • Extracts per plate: 320 • Library (2,240 extracts): 7 plates • Content/well: 0.1 ml dried-down extract Corresponds with 20 mg plant material (f.w.) *Can be supplied in dry form

Database

Public information on plant species – General • Known compounds • (Traditional) use • Edibility • Bioactivity • Toxicity – Regulatory • CITES, EU Flora & Fauna, IUCN Red List, Dutch Food and Consumer law on banned herbal products (Warenwetbesluit Kruidenmiddelen), EMA - THMP, EFSA toxic botanicals, Currently used and excluded cosmetic ingredients in IECIC China, EU banned and allowed cosmetic compounds • Plant Breeder’s Rights • Patents

Database – Taxonomy

Database – Regulatory

Database – Literature

Database – Compounds

Screening Extract Library

Screening results - I – Pharmaceutical • HTS in an anti-inflammatory activity assay – Cleaned extracts (960) in DMSO; 384-well format – A number of hits - to be validated • Anti-mycobacterium activity (TBC) – Cleaned extracts (320) in ethanol; 384-well format – One hit - to be validated

Screening results - II – Agrobiological • Activity against an important agricultural fungal disease – Cleaned extracts (960) converted to 96-well format in DMSO – Crude extracts (320) in 96-well format converted to DMSO – Several hits - to be validated – Food • Activity against an important food spoiling fungus – Cleaned extracts (960) converted to 96-well format in DMSO – Crude extracts (320) in 96-well format converted to DMSO – Several hits - to be validated

Access Extract Library

Products – Extract Library • Extracts for screening – Crude or cleaned; Single or plated • Additional extract to e.g. validate hits • Plant material • Public information from the database – Additional products • Preparation of extracts from plant species related to the “hit” • R&D - e.g. to increase the active component by breeding • Plant material for product development • Plant material for product

Commercial terms – Extracts for screening • Screening agreement • Blind screening – Extracts supplied under code • List of genera • Price per extract / plate • Success fee for hits that are pursued • Customer gets a first right to negotiate a license – Public information from the database • Access fee for available data per plant species

Contact us Dr. Jan Pen Project Manager Pompoenweg 9 2321 DK Leiden The Netherlands t: +31 6 53861090 e. jan.pen@plantenstoffen.nl