Welcome National Reference Centre (NRC) Mieke Reyniers National Plant Protection Organization (NPPO-NL) The Netherlands
NRC: part of the plant health system in the Netherlands 2
Importance of good infrastructure 3
Plant health system in the Netherlands: government • Naktuinbouw : the Netherlands Inspection Service for Horticulture • BKD : the Flower Bulb Inspection Service • NAK : the Dutch General Inspection Service • KCB : the Quality Control Bureau 4
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National Reference Centre (NRC) 7
Dutch-NPPO/NRC part of the Netherlands Food and Consumer Product Safety Authority (NVWA) 2500 fellow workers Headquartered in Utrecht Inspection offices throughout the country Wageningen: regional office + laboratories Independent agency in the Ministry of Agriculture, Nature and Food Quality Delivery agency for the Ministry of Health, Welfare and Sport. 8
NVWA covers 23 domains Export Import Hospitality industry and artisanal production Alcohol and Veterinary medicinal Meat supply chain tobacco products and Food safety Product safety Animal welfare Nature Plant Health Fertilizer Industrial production Animal by-products Microbioloy Animal testing Fish, fish products and aquaculture Animal feed European subsidies Cross compliance Crop protection Animal Health Ground-bound subsidies
Organization chart NRC National Reference Center – plant health more than a lab Head Mieke Reyniers Plant Pests Diagnostic methods Plant Diseases Anton van der Sommen Erik Souer Maikel Aveskamp - Entomology - Administration - Bacteriology - Nematology - Biosafety/-security - Mycology - Invasive plants - Molecular Biology - Virology - Centre for Monitoring vectors - Greenhouses
National Reference Center – plant health more than a lab Head Mieke Reyniers Plant Pests Diagnostic methods Plant Diseases Anton van der Sommen Erik Souer Maikel Aveskamp - Entomology - Administration - Bacteriology - Nematology - Biosafety/-security - Mycology - Invasive plants - Molecular Biology - Virology - Centre for Monitoring vectors - Greenhouses
Tasks of the NRC
Knowledge NRC has an independent, scientific based position within the NVWA Contribution to science: diagnostic methods, diagnostic protocols, taxonomy, biology and epidemiology Supervision/contribution to research projects: eg H2020 (EU), EUPHRESCO (EPPO), PPS (Min. EZK)
Knowledge Working on pest risk analysis, quick scans (EU, EPPO, EFSA) Participation in International meetings Maintenance of network (lab visits)
Methods Method development: 1) Test Development literature, third parties, own research 2) (Lab-)Validation Robust, specific, reliable 3) Implementation
Diagnosis - Clients: - NVWA inspection - Agricultural Inspection Agencies (reference samples) - Third parties Mainly focus on quarantine- organisms (risk-based)
Diagnosis - Microscopy (Conventional, IF, EM) - Biochemical tests (eg DAS-ELISA, MALDI-TOF) - In planta (e.g. Koch’s Postulates, fysiodetermination) - Molecular methods (eg RealTime PCR, Sangersequencing new: High Throughput Sequencing) Identification = Combination of at least two independent tests
Diagnosis Facilities Quarantine building Quarantine greenhouses Outdoor fields (open and contained)
Collections - Collection ~ 230k specimens of plant pests and pathogens (Reference material) - Herbarium/ pinned insects / microscopic slides - Living collection - On culture - Symptoms - Tissue and DNA
Education - Training (inspectors of NVWA, inspectors of Agricultural Inspection Agencies, EU, public networks, EUPHRESCO) - Capacity building and Twinning (eg Turkey, Vietnam, Egypt, Kenya) - Internships (applied-) universities, college
Advice NVWA, Ministry eg Risk assessments, Legislation 3 rd countries (export), elimination scenarios, … Inspection Sampling methods, risk- based inspection,… EPPO, EU, IPPC Diagnostic protocols, Methodologies, …
Supervision Labs of the Agricultural Inspection Agencies (NAK, Naktuinbouw, BKD, KCB) - Training - Advice - Auditor: ISO 17025 Quality Management implementation All labs working with Q-organisms - Auditor: 2008/61 Biosecurity implementation
Risk Assessment Potential threats: Signaling (literature/finding/third country) Quick-scan Pest Risk Analyses (PRA)
Quality Management System - ISO17025 accredited - Fixed scope (since 2014) Accreditation is limited to predefined host- matrix combinations - Flexscope (since 2017) Extension of accreditation is possible for all host-matrix combinations www.rva.nl
Biosafety & Biosecurity - Internal Make sure that organisms stay inside - External Advise on working with Q-organisms under 2008/61 - labs of the Agricultural Inspection Agencies - other phytosanitairy labs, - breeding companies, - research institutes, - universities
Goals workshop: - expanding professional network - sharing knowledge via presentations and posters - identify shared research needs
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