VITRIFICATION MARTINA FILOV REPROFIT INTERNATIONAL S.R.O. - PowerPoint PPT Presentation

THE ART of EGG VITRIFICATION MARTINA FILOVÁ REPROFIT INTERNATIONAL S.R.O.

Vitrification - principals ES= 7.4% EG/7.4% DMSO VS= 14.5% EG/14.5% DMSO/0.5M trehalose • • DS= 0.5M trehalose TS= 1,0M trehalose , T= 37⁰C

Kitazato close and open system

System &Product variety Systems: 1. Fully open system – Cryotop, Cryotech, Cryolock 2. Open cooling and close storage system – CryotopSC, OPS 3.Semi-closed cooling – Cryohook, Vitrolife, Cryotop 4.Closed thin walled , narrow capillaries- Cryotip, Cryopette 5.Carrier tools sealed into a container - High security virtrification kit, Vitrisystems -- close Media -- Kitazato Vitrolife Irvine Sage

Trustworthiness - Reliability Commercial availability ≠ relia bility

Validation in the Lab – satisfactory, adequate ??? Short time - 10 – 20 cases Eggs - immature (MI,GV) FTF after IVF Survival – main paramater ! poor development no blastulation

Kitazato - Close CLOSE - 6.11.2017- 7.2.2018 24 donors – 216 oocytes 198 warmed out Results - unsatisfactory ( inconsistent)

Is cooling and warming rate the main factor??

The magic of making bread

Factors applying to successful vitrification

Temperature

Humidity

Loading - crucial step

Kitazato – close/open CLOSE/OPEN - 21.2.2018- 20.3.2018 12 donors – 105 oocytes 105 warmed out Close Open 36/7 53/10 5,1 5,3 oo/cycle 26/36 72% 50/53 94% SR (%) 11/26 42% 44/50 88% FR (%) 8/26 28/50 30% 56% BR (%) 3/26 14/50 12% 28% UR ICSIoo (%) 3/36 14/53 9% 26% UR Vitri Oo (%)

Open system study OPEN - 10.4.2018 68 donors – 802 oocytes 437 warmed out

Results

Pregnancy rate

Biosafety All medical intervention means a certain risk to the patient, it is duty of the health service provider to make the right decision between the possible benefits and the chances of harm

Thank you!