BARCLAY OBIERO, University of Nairobi student Queen Elizabeth Scholar Summer Exchange SUPERVISOR: Dr. Helen Dimaras
Background. Introduction. Justification of the study. Histopathology techniques. Study objectives. Study methodology.
Retinoblastoma is a rare genetic cancer of the infant retina ◦ It can affect one (unilateral) or both eyes (bilateral) It is diagnosed in approximately 8,000 children each year worldwide (2). The incidence of retinoblastoma in Kenya is consistent with global published figures, at 1/17,030 live births(1). At the country’s main referral hospital, approximately 60 – 80% of ophthalmology ward beds are occupied by retinoblastoma patients(2).
The Kenyan National Retinoblastoma Strategy was formed to improve retinoblastoma care. The RbCoLab was established to provide histology services for retinoblastoma. The eyes are processed without removing sample for genetic testing, as it is not yet available in Kenya.
Surgical handling of tissues can introduce artefacts (e.g. distortion, folding, floater) which can compromise an accurate diagnosis. The information gathered will be important for quality improvement as genetic testing becomes introduced in Kenya, requiring sampling of tumor from the eyes.
Fixation Dehydration Clearing eye with tumor sampling Embedding Sectioning Staining eye without tumor sampling Are artefa facts cts produce ced when hen eye is sampled?
A well stained slide of retinoblastoma eye tissue
A disfigured retinoblastoma eye due to tumor sampling.
Broad d objectiv ctive: : To determine whether tumor sampling for genetic testing causes artefacts on histology slides. Speci cifi fic c objectives: ctives: 1.To determine the presence of artefacts and the frequency of occurrence. 2.To determine the prevalence of artefacts in sampled and un sampled eyes 3. To suggest corrective measures that minimize the frequency of artefacts in the histopathology laboratory.
This study is a descriptive retrospective study. The study is being conducted at the histopathology laboratory at The Hospital for Sick Children having obtained QI approval. The study involves the review of digital slide images,and pathology reports on eyes enucleated for retinoblastoma in the last 10 yrs.
Currently 68 digital slides have been reviewed with the assistance of a pathologist.
Data tabulated on an Excel Spreadsheet. Parameters such as the laboratory accessioning number, age of the patient in months at the time of enucleation, tumor classification listed on the accessioning form (and) tabulated. A standard Qc digital image used for comparison with rep digital images. SPSS for data analysis.
Developi veloping g Clinic inical al Canc ancer er Genetics netics Services vices in Resour source ce- 1. 1. Limit mited ed Cou ountri ries es The he Case se of Reti tinobla lasto toma a in Kenya. ya. Li Qun un He, , Luc ucy Njambi ambi et al. Developi veloping g Clinic inical al Canc ancer er Genetics netics Services vices in Resour source ce- 2. 2. Limit mited ed Cou ountries tries The he Case se of f Retin inobla lastoma a in Kenya ya .Hele len Dima maras as, , Timot imothy hy et al. Artefa facts s in Histopat topathol hology. gy. Suma umar Khan an, Manisha anisha Tijar jare et 3. 3. al. al.
Recommend
More recommend
