The Power of Small The Bio-NEMS drill *Patent pending Resonator - PowerPoint PPT Presentation

The Power of Small

The Bio-NEMS drill *Patent pending

Resonator Image obtained from MEMS & Nanotechnology Exchange. <https://www.mems-exchange.org/MEMS/what-is.html> *Patent pending

DNA Coiling *Patent pending

Biological Resonator? *Patent pending

IDEA: Mimic the chromatin’s nucleosome *Patent pending

Survey What is a Microelectromechanical System (MEMS)? NEMS 28.77 Know what a MEMS is 71.23 Do NOT know what a MEMs is Fedder, G.K. ; Carnegie Mellon Univ., Pittsburgh ; Howe, R.T. ; Tsu-Jae King Liu ; Quevy, E.P. *Patent pending

Movie Projection Disseminate Nanotechnology and its contribution to Synthetic Biology *Patent pending

Some assumptions were made: All NPs are monocrystalline and equal • (Fe 3 O 4, r=11nm and Fe 3 O 4 @SiO 2 , r= 21nm) The DNA coiled around acts as a rigid body. • The force to unbind DNA from the protein is • about 6.3 pN. 6.3 pN *Patent pending

Some assumptions were made: All NPs are monocrystalline and equal • (Fe 3 O 4, r=11nm and Fe 3 O 4 @SiO 2 , r= 21nm) The DNA coiled around acts as a rigid body. • Bio – NEMS Drill The force to unbind DNA from the protein is • about 6.3 pN. DNA mass is insignificant compared to the • NP’s. All drags are the same • Homogeneous medium • 6.3 pN The system can be reduced to a single mass • and drag *Patent pending

Lorentz force equation Amount of Nanoparticles Drag force equation The amount of Bohr magnetons per unit cell Number of paired electrons Charge Total magnetic force Total force *Patent pending

*Patent pending

The Bio-NEMS drill • Individual Components: • Magnetic Nanoparticles • Peptide • Cross-linking agent • DNA strand *Patent pending

Nanoparticle Synthesis • Co-precipitation Method • More tan 50 trials • Functionalization • Biocompatible *Patent pending

Magnetite • Superparamagnetic • 22 nm monodisperse *Patent pending

Peptide Assembly • Trial 1: SASP • Trial 2: HU • DNA – binding • Electrophoresis *Patent pending

The Bio-NEMS drill *Patent pending

Law Initiative Difficulties with the importation of reactants and biological agents! A law initiative came to be as a collaboration with the Tec Monterrey team. *Patent pending

Characterization Is this real? *Patent pending

UV Spectroscopy 260 nm Blue: Np + DNA Green: Nanoparticles

Use of plasmids for characterization of the device *Patent pending

Bio-NEMS Drill as a cloning vector Improve genetic transformation *Patent pending

*Patent pending

*Patent pending

And the Brainstorm came… *Patent pending

The Test *Patent pending

Does it Work? *Patent pending

Results BBa_K737051 Used as control and DNA in our system for a traditional transformation and a Electroporation Method 60 to 80 Colonies in our Control against 80 to 100 in the enhanced electroporation *Patent pending

The Joy of Sharing *Patent pending

Achievements Deeper Research fund Low Budget understanding for students’ project with of Synthetic projects!!! great results!!! Biology!!! *Patent pending

*Patent pending

What’s next? *Patent pending

*Patent pending

*Patent pending

IT IS THE REAL DRILL!! *Patent pending

iGEM Berlin

*Patent pending

*Patent pending

Marvellous doesn’t always come from complexity