Dataset NLS / NES Thomas Heinzinger, Ron Fechtner, Verena Burger, - PowerPoint PPT Presentation

Dataset NLS / NES Thomas Heinzinger, Ron Fechtner, Verena Burger, Paul Hager, Lena Maria Hackl Technische Universität München Fakultät für Bioinformatik Rostlab München, 28.11.2019 image taken from http://jonlieffmd.com/blog/non-immune-cells-also-combat-microbes 1

Total Number of Sequences: 787 Rostlab (TUM) | Dataset NLS / NES 2

The protein seq. originate from various species Rostlab (TUM) | Dataset NLS / NES 3

Protein Length Distribution average length: 675.8 median length: 515 Rostlab (TUM) | Dataset NLS / NES 4

Signal Occurrence Rostlab (TUM) | Dataset NLS / NES 5

DNA-binding protein involved Nucleolar protein that Signal Size regulates RNAP I in cell cycle control Rostlab (TUM) | Dataset NLS / NES 6

NLS exhibits no preferred location 7

Signal motifs influence the aa distribution Rostlab (TUM) | Dataset NLS / NES 8

The signals exhibit no preferred location 9

Data Source ● ValidNES NES entries ● NESBase NES entries ● Manually curated NES and NLS SwissProt entries ● SeqNLS NLS entries Bernhofer et. al, NLSdb—major update for database of nuclear localization signals and nuclear export signals, Nucleic Acids Research Rostlab (TUM) | Dataset NLS / NES 10

Data Source ● Manually curated NES and NLS tagged UniProt entries ● MMseqs2 redundancy reduced Bernhofer et. al, NLSdb—major update for database of nuclear localization signals and nuclear export signals, Nucleic Acids Research Rostlab (TUM) | Dataset NLS / NES 11

Protein Length Distribution, split up by NES/ NLS NES median: 376 NES mean: 507.58 NLS median: 520.5 (even number of proteins) NLS mean: 681.42 Result of two-sample Kolmogorov-Smirnov Test: statistic=0.1838, pvalue=0.0032 Rostlab (TUM) | Dataset NLS / NES 12