Synthesis of Zinc Oxide Catalyzed David Kisailus by Silicatein and - PowerPoint PPT Presentation

Mentor Synthesis of Zinc Oxide Catalyzed David Kisailus by Silicatein and Biomimetic SAMs Faculty advisor Quyen Truong Dan Morse Internship in NanoSystems, Engineering and Funding Sources Technology (INSET) 2003 National Science Foundation Contra Costa Community College The Department of Energy Major ~ Chemical/Biochemical Engineering

Axial Filament Isolation Cross-section through Tethya aurantia Buffered HF Tethya aurantia 2mm x 30µm 75% Dry Weight 2mm x 2µm (Demospongiae)

Overview � Preparation of aqueous zinc oxide precursors (zinc citrate, zinc nitrate, and zinc propionate) � Investigation of catalysis of precursors in the presence of silicatein filaments � Biomimic silicatein’s catalytic site with functionalized self-assembled monolayers on gold � Characterization of products

Zinc Oxide Synthesis Experiment � Prepared aqueous precursors (zinc nitrate, zinc propionate, zinc citrate) � Precursors were mixed with native and denatured (95°C, 90 min) protein filaments at 16°C for 24 hours � Samples were centrifuged and washed 4 times to remove unreacted precursors

Result from SEM (Scanning Electron Microscope) Analyses of Filaments Zinc oxide + native filaments Zinc oxide + denatured filaments

Inductively Coupled Plasma (ICP) Analysis of Zinc Oxide Products 0.025 0.02 0.015 0.01 0.005 0 Zinc citrate + native Zinc citrate + filaments denatured filaments

Soft Lithography : General Techniques wafer Imidizole Ink Stamp (PDMS) Hydroxyl Ink

Hydroxyl 1 µ m Imidizole

Future Work � Repeat reaction between SAMs and different semiconductor precursors � Analyze crystal structure and shape of base catalyzed vs. SAM and silicatein catalyzed zinc oxide precursors

Acknowledgements � INSET and Department of Energy Project and Internship Funding � CNSI and MRL Liu-Yen Kramer, Trevor Hirst, Nick Arnold, Al Flinck � Dan Morse, David Kisailus, James Weaver, Mark Najarian, Neal Hooker, Bonnie Bosma � Super-mentors: Mike Northen, Mel McLaurin