Safety Important Facts You Need to Know
Material Safety Data Sheet • Acronym: MSDS • Required for all HAZARDOUS materials • Regulated or enforced by OSHA • Designed to provide workers and emergency personnel w/proper procedures for handling or working w/a substance
MSDS contents • Chemical Name – Common name also given • Stability – Room temp, refrigerate, freeze • Reactivity – Do not mix w/ ,,,
MSDS contents • Physical Data – Melting or boiling point • Toxicity • Health Effects and First Aid – Inhalation – Skin contact • Storage and disposal • Spill / Leak cleanup
MSDS are meant for: • Workers who store or handle the material • Workers who use the material and may be exposed to it • Emergency workers such as fire fighters or EMT who may be exposed
Where to find an MSDS • Binder in the laboratory – Organized alphabetically • On line or from the Mfg. – Can usually request on line copies if lab does not have one • Companies are required to send with the 1 st shipment of a chemical or hazardous material
Hazardous Chemical Label • What does each area mean? – Red: Flammability – Yellow: Reactivity or instability – White: Special hazard – Blue: Health and Hazard
Hazardous Chemical label
OSHA • Occupational Safety and Health Administration • Ensures worker Safety and protection • Oversees MSDS • Most common violation they cite companies for is: – Improper chemical labeling
EPA • Environmental Protection Agency • Responsible for protecting the environment • Audits companies to verify they are not improperly disposing of materials down the drain
DOT • Department of Transportation – Need to know what they are transporting – Need copies of MSDS for hazardous materials – Need special permits and containers for hazardous materials
PPE • Personal Protection Equipment – Lab coat – Safety Glasses / Goggles – Gloves – Face shield – Respirator
Proper PPE • Closed toe shoes • No contacts • No loose or hanging clothes such as ties • Minimal jewelry • Lab coat, safety glasses and gloves most common • Glasses required for all liquid experiments
FIRES NEED 3 THINGS TO START • Oxygen (Air) • Fuel (any material that will burn) • Heat (sparks, matches, flames)
Major Causes of Fires • Carelessness with smoking and matches • Misuse of electricity (overloading a circuit or overuse of extension cords) • Improper rubbish disposal • Improper storage of flammables (such as gasoline) • Arson
EXTENSION CORDS • Avoid using extension cords whenever possible • Do not exceed specified amperage • Do not run extension cords across doorways or anywhere they can be stepped on • Do not plug one extension cord into another and never plug more than one extension cord into an outlet
GENERAL FIRE SAFETY � Keep combustibles away from heat producing devices. � Cap and store combustible liquids properly. � Never prop open emergency doors. Fire doors not only let people out, they keep fire from spreading.
In the event of a fire, stay calm and RACE… • R-rescue any patient in immediate danger • A-pull the alarm and notify other employees of the location and type of fire • C-contain the fire by closing doors and windows • E-extinguish the fire or evacuate the area
TYPES OF FIRE EXTINGUISHERS • Pressurized water extinguishers (Class A fires only) • Dry Chemical extinguishers (ABC or BC) • Carbon dioxide extinguishers (Class B and C) • Foam (or AFFF and FFFP) extinguishers • Class D extinguishers
Fire Safety • You should know… – Where the fire extinguisher is located – How to use the fire extinguisher • PASS – Pull – Aim – Squeeze – Sweep
Proper Gowning • When gowning for a clean room, the process is from top to bottom – Hairnet – Beard cover – Bunny suit – Booties – Gloves – Glasses
Clean Room Classifications • Classified by the number of particles found in the air in the lab. – The number is what your count has to be less than. • For example, a class 1,000 clean room has to have less than 1,000 particles in the air • So, which classification is the cleanest? – Class 10 or 100
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Test your knowledge • What would be the first steps if you have a spill in the lab? – Alert everyone, proper PPE, follow MSDS to clean up • What is the acronym for use of fire extinguisher? – PASS (pull, aim, squeeze, sweep)
Test Your Knowledge • What is the proper order of gowning for a clean room? – Top to bottom: hair, face, body, feet, hands • Who is responsible for employee safety? – OSHA • Who is responsible for protecting environment? – EPA
Lab Cleaning and Waste Disposal • Lab Surfaces – Should be cleaned before and after experiments – 10% bleach or ethanol most commonly used • Waste Disposal – Be sure hazardous and biohazardous materials are disposed of properly – Wash materials down the drain with copious amounts of water – Paper wrappers and paper towels go in regular trash
Sterilizing Equipment and Materials • Autoclaves: work by steam and pressure – Commonly used for equipment sterilization – Indicator tape used to verify the cycle was run • UV light – Example is goggle sanitizer – UV light causes mutation in DNA of bacteria • Bleach or ethanol – Often used to clean benches, may be used to sterilize equipment during an experiment • Flames – used to sterilize tops of tubes for microbiology
Test Your Knowledge • When should you sanitize your bench? – Before and after experiments • What equipment uses steam and pressure to sterilize equipment? – Autoclaves • Where do you discard paper towels after drying your hands? – In regular trash
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