SLIDE 1 Raw materials for Agricus compost
Ralph Noble, East Malling Research, UK
SLIDE 2 RAW MATERIALS FOR AGARICUS COMPOST
- Straw types and analysis
- Alternative carbon sources
- Poultry manure and other nitrogen sources
- Recycled water
- Gypsum
- Organic mushroom compost
- Effects on mushroom quality and moulds
SLIDE 3 STRAW PROBLEMS
- Cost – alternative uses, e.g. energy, feed
- Availability – local and every year?
- High moisture content
- Variability in composting and cropping
- Change in properties during storage
SLIDE 4
Linseed Barley Wheat Rye
SLIDE 5
Rape straw
SLIDE 6
Cotton straw
SLIDE 7
Rice straw
SLIDE 8
SLIDE 9
Wheat straw stem
SLIDE 10
Wheat straw Barley straw
SLIDE 11
Wheat straw
SLIDE 12
Barley straw
SLIDE 13 WHEAT STRAW VARIABLES
- Cultivar, spring or winter wheat
- Growing site, soil, climate
- Fertiliser (Nitrogen) applications
- Fungicides and plant growth regulators
- Harvesting method and conditions
- Storage duration and conditions
SLIDE 14 STRAW ANALYSIS METHODS
Information about organic polymers (lignin, cellulose, hemicellulose)
- Vis-near infrared spectroscopy (Vis-NIR)
- Derivative Thermogravimetry (DTG)
SLIDE 15 Transformed vis-NIR spectra of straw showing variability in composition
Moisture peaks Nitrogen Cellulose
2266
Oil 2072 Nitrogen
1514
Aromatics
SLIDE 16 Overlay of DTG curves of straw samples
mg
- 0. 0
- 0. 5
- 1. 0
- 1. 5
- 2. 0
- 2. 5
- 3. 0
mi n °C 50 100 15 0 2 00 250 30 0 35 0 40 0 45 0 50 0 550 2 4 6 8 10 12 14 16 18 2 0 22 24 26 \M Gst -2- 1 MG st- 2-1 , 3 .08 50 mg \M Gst -3- 2 MG st- 3-2 , 3 . 09 80 mg \S ola n w hea t s t raw-3 So lan wh eat st r aw-3, 3 .02 50 mg mgmin^-1
mi n 2 4 6 8 10 12 14 16 18 2 0 22 24 26 mg min^-2
2 mi n 2 4 6 8 10 12 14 16 18 2 0 22 24 26
Cellulose Lignin fraction Hemicellulose (structural)
Hemicellulose amorphous
SLIDE 17 STRAW ANALYSIS METHODS
Chemical constituents and residues
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Mineral analysis (N, P, K etc)
- Soluble carbohydrates and polyphenols
- Proteins, lipids
- pH and electrical conductivity
SLIDE 18 Cultivar Site Fenpropimorph Tebuconazole mg/kg Claire Cross 0.09 0.09 Claire DownP 0.01 0.08 Claire Lim 0.12 0.11 Malacca Cross 0.09 0.09 Malacca DownP 0.01 0.08 Malacca Lim 0.12 0.03 Richmond Cross 0.11 0.22 Richmond DownP 0.01 0.09 Richmond Lim 0.09 0.13 Tanker Cross 0.08 Tanker DownP 0.06 Tanker Lim 0.04 0.04
Fungicide residues on wheat straw
SLIDE 19 Cultivar Chlormequat, mg/kg Site Kirton Welles Cross DownP Lim Axona 1.3 Hereward 7.8 Tanker 1.7 1.3 0.7 1.0 Richmond 4.8 2.2 0.6 0.5 Claire 1.3 0.5 1.2 Malacca 1.5 0.4 1.2
Chlormequat residues on wheat straw
SLIDE 20 DIGESTABILITY
Digestability
- Enzyme digestibility (Pepsin, Pentopan, Cellulase)
(dry matter digestability and hemicellulose digestability)
(cellulose, hemicellulose, lignin etc)
SLIDE 21
- Water retention properties
(moisture content and retention)
(Tensile strength, stiffness, dynamic modulus)
SLIDE 22
Mechanical Tests on Straw
Instron tensile tests - stress, strain and elastic modulus
SLIDE 23
Straw composting experiments
SLIDE 24
Strawy Degraded
SLIDE 25
SLIDE 26 Effect of wheat cultivar on mushroom yield from windrow composts
50 100 150 200 250 300 Hereward Tanker Richmond Malacca Axona cultivar yield, kg/ tonne compost winter spring
SLIDE 27
Straw of different types - New and old
SLIDE 28
SLIDE 29
- Fig. 8 Effect of wheat cultivar and site on mushroom yield
in flask experiment composts
200 400 600 800 1000 M a l a c c a R i c h m
d T a n k e r C l a i r e Yield, g/kg compost dry weight Limnavady Crossnacreevy Downpatrick Warwick HRI wheat cultivar
Effects of wheat cultivar and growing site
SLIDE 30 Figure 4 Effect of wheat cultivar and site on % dry matter and dry matter digestibility 78 80 82 84 86 88 90 92 94 96 Malacca Richmond Tanker
% Dry Matter
18 20 22 24 26 28 30 32 Malacca Richmond Tanker
% Dry Matter Digestibility
Limavady Crossnacreevy Downpatrick Warwick HRI Wheat cultivar
SLIDE 31 WHEAT STRAW ANALYSIS AND MUSHROOM CROPPING
- 20% mushroom yield difference between ‘best’ and ‘worst’
straw batches
- Growing site, N fertiliser and storage influenced wheat
straw properties and mushroom cropping
- Barley, rape and rye straw can all be used
(at least 25%)
SLIDE 32 WHEAT STRAW ANALYSIS AND MUSHROOM CROPPING
- Wheat straw cultivar only had a small influence on straw
properties and mushroom cropping
- No effects of fungicide (tebuconazle) or plant growth
regulator residues on spawn-run or yield
- No relationship between mechanical properties and
mushroom yield
SLIDE 33 WHEAT STRAW ANALYSIS AND MUSHROOM CROPPING
* dry matter * dry matter digestability (pepsin and cellulase) * ratio of cellulose and hemi-cellulose to lignin (DTG) produced a higher mushroom yield than straw with low values
SLIDE 34 STRAW QUESTIONS AND FUTURE
- Is wheat straw best or should other straw types or blends
be used?
- Is horse manure better than only straw?
- What are the key properties of straw?
- How can we adapt the composting process according to
straw analysis?
SLIDE 35 ALTERNATIVE MATERIALS PROBLEMS
- Cost – alternative uses, e.g. energy, feed
- Local availability year round
- High water content, low available C or N
- Variability in composting and cropping
- Change in properties during storage
- EU waste legislation
SLIDE 36
Tomato, pepper and cucumber haulms
SLIDE 37
Vegetable wastes
SLIDE 38
SLIDE 39
SLIDE 40
SLIDE 41
Separated spent mushroom compost
SLIDE 42
Separated spent mushroom compost
SLIDE 43
Broiler poultry manure
SLIDE 44
SLIDE 45
Recycled ‘goody’ water
SLIDE 46
SLIDE 47
- Fig. 37 Relationship between electrical conductivity and dry
matter content of goody water samples y = 1.0849x + 0.2368 R2 = 0.928 5 10 15 20 25 30 35 5 10 15 20 25 30 EC ms/cm dry matter, %
SLIDE 48
SLIDE 49 Pig manure and slurry Cattle slurry Fish by-products Shellfish wastes Wool waste
SLIDE 50
Cocoa meal
SLIDE 51
Anaerobic digestion
SLIDE 52 PAS 110:2010 Specifi cation for whole digestate, separated liquor and separated fi bre derived from the anaerobic digestion of source-segregated biodegradable materials
SLIDE 53
Ammonium sulphate
SLIDE 54
SLIDE 55 50 100 150 200 250 300
wheat barley rye +carpet +SMC + veg inorgan.
Yield, g/kg compost fresh wt. Bench Large
LSD (P = 0.05) 100% Straw/poultry manure 75% Straw/poultry manure
+wool
SLIDE 56 Effects of compost ingredients on mushroom quality
- Shelf life
- Dry matter content
- Firmness/ Density
- Whiteness/ Bruisability
- Flavour
SLIDE 57 Trichoderma aggressivum 23443B
SLIDE 59 Effect of moulds on mushroom yield in different composts
100 200 300 400 500 600 Normal Dry Wet Mature Strawy Yield, g/kg Control Penicillium Trichoderma
SLIDE 60 Agricultural gypsum
citric acid by-product gypsum
No gypsum
SLIDE 61 PAS 109:2013
Specification for the production of reprocessed gypsum from waste plasterboard
SLIDE 62 ALTERNATIVE CARBON, NITROGEN AND GYPSUM SOURCES
- Glasshouse and vegetable wastes, separated SMC and
animal manures/ by-products usable
- Digestates are a possible ingredient
- Plasterboard and citric acid gypsum,
ammonium sulphate + chalk can be used
- EU legislation interferes with some materials
- Effects of raw materials on yield, quality and compost
moulds need to be considered
SLIDE 63 ALTERNATIVE MATERIALS – QUESTIONS AND FUTURE
- Do we need alternatives to straw, horse manure and
poultry manure?
- What are the implications for organic compost?
- EU waste legislation – collaboration?
- Is further research needed?
SLIDE 64 Raw materials for Agricus compost
Ralph Noble, East Malling Research, UK