PARTNER K Cell irradiation experiments Inform models of: Tumour - PDF document

Karen Kirkby & Raj Jena Ki kb & R j J PARTNER K

• Cell irradiation experiments • Inform models of: • Tumour response to radiation • Different types of radiation • Different fractionation patterns • Efecfts of chemotherapeutic agents Efecfts of chemotherapeutic agents • Clinical trials • Tissue models

Cell irradiation experiments Cell irradiation experiments • Targeted single cell irradiation • Precisely counted single ions (H-Ar) • Sub-cellular targeting accuracy (10nm) S b ll l t ti (10 ) • High throughput (100,000 cellls/ hr) requires scanned focused beam • Ion beam analysis of “living” cells in culture • Sub-cellular spatial resolution (100nm) • Elemental mapping using scanned beam Elemental mapping using scanned beam • Horizontal sample orientation is highly desirable • Liquid culture media q • Happy cells! • Add chemotherapeutic agents

And of course we need a housing for this equipment … q p

OPTICAL DETECTION parfocal image planes END-STATION widefield excitation widefield environment detection control t l laser objectives scanning scanning f focus wide throat widefield stage g t trans- illumination beam focusing focusing and scanning

Experiments Experiments • Standard cell lines • • protons and ions protons and ions • cell cycle phase • Patient specific cell lines (TIC) 32 – GBM(12) Patient specific cell lines (TIC) 32 – GBM(12) • Protons and ions • Radio-sensitivity – differentiation cascade Radio sensitivity differentiation cascade • Effects of chemotherapeutic agents /hypoxia • Effects of chemotherapeutic agents (linked into clinical trial) • Effects of hypoxia for protons and different ions

Training Objectives Training Objectives • Standard cell lines • • Cell handling Cell handling • Use of irradiation facilities • Mathematical modelling Mathematical modelling • Patient specific cell lines (TIC) 32 – GBM(12) • Cell handling Cell handling • modelling • Chemotherapeutic agents /hypoxia • Chemo handling / dose escalation • Hypoxic cell clamp

Migrating glioma cell Migrating glioma cell •

• Patient -specific glioma cells in spheroid form

Colin Watts G12 TIC cells ll G12 TIC

Conclusions Conclusions • Proven track record of bringing clinical research and basic science together g • “Toolbox” of modelling techniques with innovative applications, which complements existing radio-biological modelling • Full spectrum of research training opportunities for patient specific radiation biology experiments